Determination of antifungal spectrum and evaluation of antifungal activity of Trichoderma viride B3 strain

doi:10.3969/j.issn.1004-1524.20231115

Acta Agriculturae Zhejiangensis ›› 2024, Vol. 36 ›› Issue (10): 2257-2263.DOI: 10.3969/j.issn.1004-1524.20231115

• Plant Protection • Previous Articles Next Articles

Determination of antifungal spectrum and evaluation of antifungal activity of Trichoderma viride B3 strain

MA Lijun(), WU Nana, XU Bingliang, LIU Jia, ZHANG Shuwu^*()

Engineering Laboratory for Biological Control of Crop Diseases and Pests, College of Plant Protection, Gansu Agricultural University, Lanzhou 730070, China

Received:2023-09-15 Online:2024-10-25 Published:2024-10-30

Abstract

Abstract:

In order to explore the antifungal spectrum and antifungal activity of Trichoderma viride B3 strain, the confrontation culture and microscopic observation were used to determine and clarify the antifungal spectrum and mechanism of T. viride B3 strain. The results showed that T. viride B3 strain had antagonistic effects against 8 plant pathogenic fungi. It had the strongest inhibition effect on the colony growth of Monilia spp. and Botrytis cinerea among the 8 plant pathogenic fungi, with the growth inhibition rates of 90.8% and 88.9%, respectively, and the antagonistic level went to Grade I; while it had the weakest inhibition effect on the growth of Alternaria mali, with the inhibition rate of 48.2% and the antagonistic level went to Grade Ⅲ. The observation of confrontation culture showed that, when T. viride B3 strain and Monilia spp. contacted with each other, T. viride B3 strain quickly occupied the nutritional growth space of the pathogenic fungi, the contact between colonies and pathogen colonies made pathogen hyphae entangled, expanded, contracted and dissolved. In conclusion, T. viride B3 strain had antagonistic effect against Monilia sp. and B. cinerea.

Key words: Trichoderma viride, plant pathogen, antagonism, growth inhibition rate

CLC Number:

S476.12

MA Lijun, WU Nana, XU Bingliang, LIU Jia, ZHANG Shuwu. Determination of antifungal spectrum and evaluation of antifungal activity of Trichoderma viride B3 strain[J]. Acta Agriculturae Zhejiangensis, 2024, 36(10): 2257-2263.

Figures/Tables 3

Fig.1 Observation the antagonism of Trichoderma viride B3 strain against 8 plant pathogenic fungi I, II, III show the confrontation culture of T. viride B3 strain with 8 plant pathogens, the single pathogen (control) and the junction of two colonies, respectively. A-H show the confrontation culture results of T. viride B3 with Monilia sp., Alternaria alternata f. sp. mali, Valsa mali, Fusarium oxysporum, Rhizoctonia solani, Botrytis cinerea, Valsa ambiens, Alternaria mali, respectively.

Table 1 Growth inhibition rates of Trichoderma viride B3 against 8 plant pathogenic fungi

病原菌 Pathogens	处理菌落半径 Colony radius of treatment/mm	对照菌落半径 Colony radius of control/mm	生长抑制率 Growth inhibition rate/%
丛梗孢Monilia sp.	5.1	30.8	90.8±0.2 a
灰葡萄孢Botrytis cinerea	5.7	31.2	88.9±0.2 a
苹果黑腐皮壳Valsa mali	7.6	28.3	80.2±0.3 b
立枯丝核菌Rhizoctonia solani	7.7	27.8	79.5±0.4 b
尖孢镰孢Fusarium oxysporum	9.6	27.4	71.5±0.5 c
梨黑腐皮壳Valsa ambiens	13.6	25.6	52.0±0.5 d
链格孢苹果专化型	14.5	27.1	51.2±0.5 de
Alternaria alternata f. sp. mali
苹果链格孢Alternaria mali	12.3	21.4	48.2±0.5 e

Fig.2 Microscopic observation of the parasitic process of Trichoderma viride B3 against Monilia sp. A, B, C, D, E, F show the microscopic observation results of T. viride B3 strain’s parasitic process on Monilia sp. on the 3rd, 4th, 5th, 6th, 7th, 8th days of confrontation culture, respectively. G and H show the normal hyphae of T. viride B3 and Monilia sp., respectively. I and J show the adhesion and entanglement of Monilia sp. mycelium after the interaction between T. viride B3 strain and Monilia sp. mycelium. K and L show the expansion and contraction of Monilia sp. mycelium after the interaction between the two strains. The scales in A-F and G-L are 2 mm and 20 μm, respectively.

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Determination of antifungal spectrum and evaluation of antifungal activity of Trichoderma viride B3 strain

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