A method for rapidly preparing the karyotype of Trionychidae species by minimally invasive blood sampling

doi:10.3969/j.issn.1004-1524.20240311

Acta Agriculturae Zhejiangensis ›› 2025, Vol. 37 ›› Issue (1): 32-38.DOI: 10.3969/j.issn.1004-1524.20240311

• Animal Science • Previous Articles Next Articles

A method for rapidly preparing the karyotype of Trionychidae species by minimally invasive blood sampling

GE Jianhui¹^,²(), GUAN Xinshuai¹, LIU Jindian¹, REN Jindong¹, XU Xiaojun¹^,^*()

1. Institute of Hydrobiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
2. College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, Zhejiang, China

Received:2024-04-01 Online:2025-01-25 Published:2025-02-14

Abstract

Abstract:

In order to establish a minimally invasive chromosome karyotype preparation method for Trionychidae species, a combination of blood drawing stimulation and in vivo injection of phytohemagglutinin (PHA) on Pelodiscus sinensis was used to promote hematopoietic cell proliferation. The blood collected from the spinal canal was used as initial tissue material. Chromosome preparation was carried out by colchicine treatment and leukocyte isolation, and karyotype analysis was performed for the mitotic metaphase cells. The results showed that adequate chromosomes of mitotic metaphase could be obtained by this method. In 33 pairs of chromosomes, six were large chromosomes, and the rest 27 were small chromosomes. The karyotype formula was 2n=66=4M+6SM+2T+54m. This study would provide a minimally invasive, simple and fast karyotype method of Trionychidae species, which could provide a powerful tool for the genetic breeding and germplasm identification of Trionychidae species.

Key words: Trionychidae species, minimally invasive, karyotype

CLC Number:

S917

GE Jianhui, GUAN Xinshuai, LIU Jindian, REN Jindong, XU Xiaojun. A method for rapidly preparing the karyotype of Trionychidae species by minimally invasive blood sampling[J]. Acta Agriculturae Zhejiangensis, 2025, 37(1): 32-38.

Figures/Tables 5

References 35

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染色体数量 Number of chromosome	≤64	65	66	67	≥68	总和 Sum
分裂相数量 Number of metaphase	8	14	35	2	1	60
所占百分比 Percentage of metaphase/%	13.33	23.33	58.33	3.33	1.68	100.00

染色体数量 Number of chromosome	≤64	65	66	67	≥68	总和 Sum
分裂相数量 Number of metaphase	8	14	35	2	1	60
所占百分比 Percentage of metaphase/%	13.33	23.33	58.33	3.33	1.68	100.00

染色体序号 Chromosome No.	长度 Length/μm	臂比指数 Arm ratio	染色体类型 Chromosome type
1	11.995	1.499	M
2	8.267	1.033	M
3	7.120	1.883	SM
4	5.035	1.874	SM
5	5.720	2.305	SM
6	5.055	9.215	T

染色体序号 Chromosome No.	长度 Length/μm	臂比指数 Arm ratio	染色体类型 Chromosome type
1	11.995	1.499	M
2	8.267	1.033	M
3	7.120	1.883	SM
4	5.035	1.874	SM
5	5.720	2.305	SM
6	5.055	9.215	T

A method for rapidly preparing the karyotype of Trionychidae species by minimally invasive blood sampling

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