Epidemiological investigation and evaluation of purification strategies for avian leukosis in local chicken breeds in Zhejiang Province of China

doi:10.3969/j.issn.1004-1524.20240792

Acta Agriculturae Zhejiangensis ›› 2025, Vol. 37 ›› Issue (4): 790-799.DOI: 10.3969/j.issn.1004-1524.20240792

• Animal Science • Previous Articles Next Articles

Epidemiological investigation and evaluation of purification strategies for avian leukosis in local chicken breeds in Zhejiang Province of China

NI Zheng¹(), LU Lei², ZHU Yinchu¹, CHEN Liu¹, LI Xinji³, YE Weicheng¹, YUN Tao¹, HUA Jionggang¹, FU Yuan¹, ZHANG Cun¹^,^*()

1. Institute of Animal Husbandry and Veterinary Medicine, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
2. Zhejiang Zhenming Poultry Industry Co., Ltd., Ningbo 315632, Zhejiang, China
3. College of Veterinary Medicine, Henan University of Science and Technology, Luoyang 471023, Henan, China

Received:2024-09-09 Online:2025-04-25 Published:2025-05-09

Abstract

Abstract:

To assess the prevalence of avian leukosis virus (ALV) in local poultry breeds in Zhejiang Province of China and evaluate the effectiveness of a purification program, a comprehensive study was conducted. From 2021 to 2024, a total of 163 453 samples, including meconium, egg white, and plasma, were collected from 6 indigenous poultry breeds in Zhejiang. The presence of ALV p27 antigen was determined using an ELISA kit, and a three-generation purification process was applied to evaluate its efficacy. Positive samples were subjected to isolation, identification, and histopathological examination. The genetic evolution of the isolated ALV strains was analyzed by amplifying the gp85 gene. It was revealed that the ALV infection was prevalent across all 6 local breeds, with an average p27 antigen positive rate of 10.02%. Notably, the white-eared yellow chicken breed exhibited the highest positive rate of 43.87%. Following the three-generation purification process, there was a significant reduction in the positive rate across all samples. However, variations were observed among breeds. The Jiangshan black bone chicken demonstrated the most effective purification, as no detectable p27 antigen in egg white and plasma samples after three generations. Two distinct strains of ALV (ZJU2401 and ZJU2402) were isolated from the positive samples and were classified as subgroup J based on phylogenetic analysis. The study verified that the purification strategy was effective, and these findings could provide a foundation for the management of avian leukosis in local poultry breeds in Zhejiang Province, and offer valuable data support for further purification and preventive measures.

Key words: avian leukosis, evaluation of purification effect, gp85 gene, genetic evolution

CLC Number:

S852.659.1

NI Zheng, LU Lei, ZHU Yinchu, CHEN Liu, LI Xinji, YE Weicheng, YUN Tao, HUA Jionggang, FU Yuan, ZHANG Cun. Epidemiological investigation and evaluation of purification strategies for avian leukosis in local chicken breeds in Zhejiang Province of China[J]. Acta Agriculturae Zhejiangensis, 2025, 37(4): 790-799.

Figures/Tables 9

References 26

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毒株Strain	亚群Subgroup	登录号GenBank accession No.	年份Year	地域Area
NX0101	J	DQ115805	2016	中国China
NGA_VX23	J	MK104146	2018	尼日利亚Nigeria
J-SDAU12-737	J	KM873195	2015	中国China
GDQJ-4	J	KU170199	2016	中国China
HPRS103	J	Z46390	2006	英国UK
GD1804J6	J	MZ856317	2021	中国China
JS17DX01	K	MT783282	2021	中国China
SD20LH01	K	OP035379	2023	中国China
CH/JXND2101	K	OR670961	2024	中国China
CH/JXTH2202	K	OR670967	2024	中国China
JS13LY19	K	MG770235	2018	中国China
JS11C1	K	KF746200	2014	中国China
JS13-DX5	K	KF999961	2014	中国China
MQNCSU	A	DQ365814	2016	英国UK
RAV-1	A	MF926337	2019	美国USA
SDAU09E1	A	HM452341	2016	中国China
SDAU09E2	A	HM452342	2016	中国China
DPRE32	A	KM434201	2014	印度India
SDAU09E3	B	JF826241	2012	中国China
SDAU09C2	B	HM446005	2016	中国China
GX14FF03	B	KU923579	2016	中国China
HLJA0202	B	MW804649	2022	中国China
ALVE-B10	E	KC610516	2013	加拿大Canada
ev-21	E	KY235336\|	2016	中国China
AF229	E	MF817821	2016	美国USA
RAV0	E	MF817822	2017	美国USA
ev-I	E	AY013303	2001	美国USA
SDO501	E	EF467236	2007	中国China

毒株Strain	亚群Subgroup	登录号GenBank accession No.	年份Year	地域Area
NX0101	J	DQ115805	2016	中国China
NGA_VX23	J	MK104146	2018	尼日利亚Nigeria
J-SDAU12-737	J	KM873195	2015	中国China
GDQJ-4	J	KU170199	2016	中国China
HPRS103	J	Z46390	2006	英国UK
GD1804J6	J	MZ856317	2021	中国China
JS17DX01	K	MT783282	2021	中国China
SD20LH01	K	OP035379	2023	中国China
CH/JXND2101	K	OR670961	2024	中国China
CH/JXTH2202	K	OR670967	2024	中国China
JS13LY19	K	MG770235	2018	中国China
JS11C1	K	KF746200	2014	中国China
JS13-DX5	K	KF999961	2014	中国China
MQNCSU	A	DQ365814	2016	英国UK
RAV-1	A	MF926337	2019	美国USA
SDAU09E1	A	HM452341	2016	中国China
SDAU09E2	A	HM452342	2016	中国China
DPRE32	A	KM434201	2014	印度India
SDAU09E3	B	JF826241	2012	中国China
SDAU09C2	B	HM446005	2016	中国China
GX14FF03	B	KU923579	2016	中国China
HLJA0202	B	MW804649	2022	中国China
ALVE-B10	E	KC610516	2013	加拿大Canada
ev-21	E	KY235336\|	2016	中国China
AF229	E	MF817821	2016	美国USA
RAV0	E	MF817822	2017	美国USA
ev-I	E	AY013303	2001	美国USA
SDO501	E	EF467236	2007	中国China

品种 Variety	日龄 Age/d	阳性率Positive rate/%
品种 Variety	日龄 Age/d	公鸡Rooster	母鸡Hen	平均Averagel
仙居鸡Xianju chicken	240	10.75(20/186)	2.71 (33/1 209)	3.80 (53/1 395)
萧山鸡Xiaoshan chicken	220	6.91 (15/217)	6.65 (104/1 564)	6.68 (119/1 781)
江山乌骨鸡Jiangshan silky chicken	210	2.50 (2/80)	3.85 (7/182)	3.44 (9/262)
矮脚黄鸡Aijiao yellow chicken	220	20.95 (22/105)	11.60 (110/948)	12.54 (132/1 053)
麻黄土鸡Mahuang indigenous chicken	225	6.33 (5/79)	10.42 (45/432)	9.78 (50/511)
白耳黄鸡Baier yellow chicken	240	47.13 (41/87)	42.99 (138/321)	43.87 (179/408)
合计Total	_	13.93 (105/754)	9.39 (437/4 656)	10.02 (542/5 410)

品种 Variety	日龄 Age/d	阳性率Positive rate/%
品种 Variety	日龄 Age/d	公鸡Rooster	母鸡Hen	平均Averagel
仙居鸡Xianju chicken	240	10.75(20/186)	2.71 (33/1 209)	3.80 (53/1 395)
萧山鸡Xiaoshan chicken	220	6.91 (15/217)	6.65 (104/1 564)	6.68 (119/1 781)
江山乌骨鸡Jiangshan silky chicken	210	2.50 (2/80)	3.85 (7/182)	3.44 (9/262)
矮脚黄鸡Aijiao yellow chicken	220	20.95 (22/105)	11.60 (110/948)	12.54 (132/1 053)
麻黄土鸡Mahuang indigenous chicken	225	6.33 (5/79)	10.42 (45/432)	9.78 (50/511)
白耳黄鸡Baier yellow chicken	240	47.13 (41/87)	42.99 (138/321)	43.87 (179/408)
合计Total	_	13.93 (105/754)	9.39 (437/4 656)	10.02 (542/5 410)

品种 Variety	样品类型 Sample type	阳性率Positive rate/%
品种 Variety	样品类型 Sample type	1世代Generation 1	2世代Generation 2	3世代Generation 3
仙居鸡	胎粪Meconium	3.36(175/5 215)	2.38(118/4 968)	0.52(26/5 026)
Xianju chicken	蛋清Egg white	1.29(24/1 858)	0.32(6/1 882)	0.58(7/1 212)
	血浆Plasma	3.96(72/1 818)	1.08(16/1 480)	0.57(9/1 584)
萧山鸡	胎粪Meconium	5.39(336/6 237)	1.58(95/6 031)	1.67(100/5 977)
Xiaoshan chicken	蛋清Egg white	2.20(60/2 727)	0.68(12/1 772)	0.07(1/1 460)
	血浆Plasma	3.13(60/1 920)	4.68(80/1 710)	1.44(26/1 807)
江山乌骨鸡	胎粪Meconium	3.17(175/5 527)	2.18(116/5 324)	0.20(11/5 621)
Jiangshan silky chicken	蛋清Egg white	3.54(64/1 808)	1.72(32/1 864)	0(0/2 020)
	血浆Plasma	1.61(26/1 612)	0(0/1 550)	0(0/1 920)
矮脚黄鸡	胎粪Meconium	15.42(931/6 036)	6.97(430/6 167)	2.56(156/6 085)
Aijiao yellow chicken	蛋清Egg white	5.36(102/1 902)	2.19(44/2 012)	1.87(24/1 283)
	血浆Plasma	17.78(312/1 755)	7.36(132/1 793)	4.55(80/1 760)
麻黄土鸡	胎粪Meconium	7.68(449/5 843)	5.98(333/5 573)	0.33(18/5 396)
Mahuang indigenous chicken	蛋清Egg white	8.44(128/1 516)	4.70(85/1 810)	0.31(6/1 938)
	血浆Plasma	5.00(63/1 260)	4.35(60/1 380)	1.28(20/1 560)
白耳黄鸡	胎粪Meconium	18.80(1059/5 634)	10.13(597/5 894)	1.21(69/5 703)
Baier yellow chicken	蛋清Egg white	14.47(245/1 701)	8.85(132/1 548)	1.85(33/1 782)
	血浆Plasma	3.80(54/1 422)	2.27(30/1 320)	0(0/1 450)

Epidemiological investigation and evaluation of purification strategies for avian leukosis in local chicken breeds in Zhejiang Province of China

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