Research progress on the function of galactinol synthase gene family in plants

doi:10.3969/j.issn.1004-1524.20240115

Acta Agriculturae Zhejiangensis ›› 2025, Vol. 37 ›› Issue (8): 1817-1824.DOI: 10.3969/j.issn.1004-1524.20240115

• Review • Previous Articles

Research progress on the function of galactinol synthase gene family in plants

LIU Yan(), LIN Tianbao, LYU Zhiqiang()

Institute of Sericulture and Tea Research, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2024-01-30 Online:2025-08-25 Published:2025-09-03
Contact: LYU Zhiqiang

Abstract

Abstract:

Raffinose family oligosaccharides (RFOs) are widespread carbohydrates functioning in storage and transport in plants. Galactinol synthase (GolS), a key rate-limiting enzyme in raffinose biosynthesis, is involved in regulating carbon storage, phloem transport, osmotic adjustment, and stress signal transduction, and also plays a crucial role in plant growth, development, and stress responses. In the present paper, we review the research progress on the structural characteristics and biological functions of plant GolS gene family, and summarize its roles in plant assimilate transport, seed development, and cell secondary wall formation, as well as its ability to enhance plant stress resistance by regulating cellular osmotic pressure and antioxidant capacity. Future research is prospected to focus on exploring the regulatory mechanisms underlying GolS gene expression and their potential in crop improvement and stress-resistant breeding, which may provide theoretical support and technical references for agricultural production.

Key words: raffinose family oligosaccharides (RFOs), galactinol synthase, stress tolerance

CLC Number:

Q945

LIU Yan, LIN Tianbao, LYU Zhiqiang. Research progress on the function of galactinol synthase gene family in plants[J]. Acta Agriculturae Zhejiangensis, 2025, 37(8): 1817-1824.

References 59

[1]	PETERBAUER T, RICHTER A. Biochemistry and physiology of raffinose family oligosaccharides and galactosyl cyclitols in seeds[J]. Seed Science Research, 2001, 11: 185-197.
[2]	CASTILLO E M, DE LUMEN B O, REYES P S, et al. Raffinose synthase and galactinol synthase in developing seeds and leaves of legumes[J]. Journal of Agricultural and Food Chemistry, 1990, 38(2): 351-355.
[3]	AYRE B G, KELLER F, TURGEON R. Symplastic continuity between companion cells and the translocation stream: long-distance transport is controlled by retention and retrieval mechanisms in the phloem[J]. Plant Physiology, 2003, 131(4): 1518-1528.
[4]	SHEEN J, ZHOU L, JANG J C. Sugars as signaling molecules[J]. Current Opinion in Plant Biology, 1999, 2(5): 410-418.
[5]	SENGUPTA S, MUKHERJEE S, BASAK P, et al. Significance of galactinol and raffinose family oligosaccharide synthesis in plants[J]. Frontiers in Plant Science, 2015, 6: 656.
[6]	张古文, 胡齐赞, 徐盛春, 等. 菜用大豆籽粒发育过程中蔗糖积累及相关酶活性的研究[J]. 浙江农业学报, 2012, 24(6): 1015-1020.
	ZHANG G W, HU Q Z, XU S C, et al. Study on sucrose accumulation and enzyme activities involved in sucrose metabolism in developing seeds of vegetable soybean[J]. Acta Agriculturae Zhejiangensis, 2012, 24(6): 1015-1020. (in Chinese with English abstract)
[7]	SENGUPTA S, MUKHERJEE S, PARWEEN S, et al. Galactinol synthase across evolutionary diverse taxa: Functional preference for higher plants?[J]. FEBS Letters, 2012, 586(10): 1488-1496.
[8]	YIN Y B, CHEN H L, HAHN M G, et al. Evolution and function of the plant cell wall synthesis-related glycosyltransferase family 8[J]. Plant Physiology, 2010, 153(4): 1729-1746.
[9]	DAI H, ZHU Z, WANG Z, et al. Galactinol synthase 1 improves cucumber performance under cold stress by enhancing assimilate translocation[J]. Horticulture Research, 2022, 9: uhab063.
[10]	TAJI T, OHSUMI C, IUCHI S, et al. Important roles of drought- and cold-inducible genes for galactinol synthase in stress tolerance in Arabidopsis thaliana[J]. The Plant Journal, 2002, 29(4): 417-426.
[11]	YOU J, WANG Y Y, ZHANG Y J, et al. Genome-wide identification and expression analyses of genes involved in raffinose accumulation in sesame[J]. Scientific Reports, 2018, 8(1): 4331.
[12]	FALAVIGNA V D S, PORTO D D, MIOTTO Y E, et al. Evolutionary diversification of galactinol synthases in Rosaceae: adaptive roles of galactinol and raffinose during apple bud dormancy[J]. Journal of Experimental Botany, 2018, 69(5): 1247-1259.
[13]	FAN Y H, YU M N, LIU M, et al. Genome-wide identification, evolutionary and expression analyses of the GALACTINOL SYNTHASE gene family in rapeseed and tobacco[J]. International Journal of Molecular Sciences, 2017, 18(12): 2768.
[14]	ZHOU J, YANG Y, YU J, et al. Responses of Populus trichocarpa galactinol synthase genes to abiotic stresses[J]. Journal of Plant Research, 2014, 127(2): 347-358.
[15]	JING Q K, CHEN A R, LV Z Y, et al. Systematic analysis of galactinol synthase and raffinose synthase gene families in potato and their expression patterns in development and abiotic stress responses[J]. Genes, 2023, 14(7): 1344.
[16]	ZHOU Y, LIU Y, WANG S S, et al. Molecular cloning and characterization of galactinol synthases in Camellia sinensis with different responses to biotic and abiotic stressors[J]. Journal of Agricultural and Food Chemistry, 2017, 65(13): 2751-2759.
[17]	HANNAH M A, ZUTHER E, BUCHEL K, et al. Transport and metabolism of raffinose family oligosaccharides in transgenic potato[J]. Journal of Experimental Botany, 2006, 57(14): 3801-3811.
[18]	MCCASKILL A, TURGEON R. Phloem loading in Verbascum phoeniceum L. depends on the synthesis of raffinose-family oligosaccharides[J]. Proceedings of the National Academy of Sciences of the United States of America, 2007, 104(49): 19619-19624.
[19]	MA S, SUN L L, SUI X L, et al. Phloem loading in cucumber: combined symplastic and apoplastic strategies[J]. The Plant Journal, 2019, 98(3): 391-404.
[20]	韦玉霞. 园艺作物中棉子糖系列寡糖(RFO)研究进展[J]. 园艺与种苗, 2017, 37(9): 46-48.
	WEI Y X. Research progress of raffinose family oligosaccharides(RFO) in horticultural crops[J]. Horticulture & Seed, 2017, 37(9): 46-48. (in Chinese with English abstract)
[21]	张瑞腾, 吕建春, 周梦迪, 等. 甜瓜肌醇半乳糖苷合成酶基因CmGAS1的表达与功能分析[J]. 园艺学报, 2018, 45(10): 1929-1940.
	ZHANG R T, LÜ J C, ZHOU M D, et al. Expression and function analysis of CmGAS1 in melon[J]. Acta Horticulturae Sinica, 2018, 45(10): 1929-1940. (in Chinese with English abstract)
[22]	JING Y, LANG S R, WANG D M, et al. Functional characterization of galactinol synthase and raffinose synthase in desiccation tolerance acquisition in developing Arabidopsis seeds[J]. Journal of Plant Physiology, 2018, 230: 109-121.
[23]	LI X, ZHUO J J, JING Y, et al. Expression of a GALACTINOL SYNTHASE gene is positively associated with desiccation tolerance of Brassica napus seeds during development[J]. Journal of Plant Physiology, 2011, 168(15): 1761-1770.
[24]	SALVI P, SAXENA S C, PETLA B P, et al. Differentially expressed galactinol synthase(s) in chickpea are implicated in seed vigor and longevity by limiting the age induced ROS accumulation[J]. Scientific Reports, 2016, 6: 35088.
[25]	LE H, NGUYEN N H, TA D T, et al. CRISPR/Cas9-mediated knockout of galactinol synthase-encoding genes reduces raffinose family oligosaccharide levels in soybean seeds[J]. Frontiers in Plant Science, 2020, 11: 612942.
[26]	GANGOLA M P, JAISWAL S, KANNAN U, et al. Galactinol synthase enzyme activity influences raffinose family oligosaccharides (RFO) accumulation in developing chickpea (Cicer arietinum L.) seeds[J]. Phytochemistry, 2016, 125: 88-98.
[27]	JANG J H, SHANG Y, KANG H K, et al. Arabidopsis galactinol synthases 1 (AtGOLS1) negatively regulates seed germination[J]. Plant Science, 2018, 267: 94-101.
[28]	UNDA F, KIM H, HEFER C, et al. Altering carbon allocation in hybrid poplar (Populus alba × grandidentata) impacts cell wall growth and development[J]. Plant Biotechnology Journal, 2017, 15(7): 865-878.
[29]	范洁. 木薯肌醇半乳糖苷合成酶基因MeGolS5的抗旱功能研究[D]. 海口: 海南大学, 2015.
	FAN J. Study on drought resistance of cassava inositol galactoside synthase gene MeGolS5[D]. Haikou: Hainan University, 2015. (in Chinese with English abstract)
[30]	张军, 邱爽, 何佳琦, 等. 大豆GmGolS基因植物表达载体构建及烟草遗传转化[J]. 齐齐哈尔大学学报(自然科学版), 2020, 36(6): 22-25.
	ZHANG J, QIU S, HE J Q, et al. Plant expression vector construction and tobacco genetic transformation of soybean GmGolS gene[J]. Journal of Qiqihar University(Natural Science Edition), 2020, 36(6): 22-25. (in Chinese with English abstract)
[31]	刘爱丽, 魏梦园, 黎冬华, 等. 芝麻肌醇半乳糖苷合成酶基因SiGolS6的克隆及功能分析[J]. 中国农业科学, 2020, 53(17): 3432-3442.
	LIU A L, WEI M Y, LI D H, et al. Cloning and function analysis of sesame galactinol synthase gene SiGolS6 in Arabidopsis[J]. Scientia Agricultura Sinica, 2020, 53(17): 3432-3442. (in Chinese with English abstract)
[32]	SHIKAKURA Y, OGUCHI T, YU X, et al. Transgenic poplar trees overexpressing AtGolS2, a stress-responsive galactinol synthase gene derived from Arabidopsis thaliana, improved drought tolerance in a confined field[J]. Transgenic Research, 2022, 31(4): 579-591.
[33]	SELVARAJ M G, ISHIZAKI T, VALENCIA M, et al. Overexpression of an Arabidopsis thaliana galactinol synthase gene improves drought tolerance in transgenic rice and increased grain yield in the field[J]. Plant Biotechnology Journal, 2017, 15(11): 1465-1477.
[34]	LIU Y D, ZHANG L, CHEN L J, et al. Molecular cloning and expression of an encoding galactinol synthase gene (AnGolS1) in seedling of Ammopiptanthus nanus[J]. Scientific Reports, 2016, 6: 36113.
[35]	孙海伟. 沙冬青肌醇半乳糖苷合成酶(AmGS)基因转化类茶植物红叶石楠的研究[D]. 泰安: 山东农业大学, 2012.
	SUN H W. Study on transformation of ammopiptanthus mongolicus galactosidase (AmGS) gene into tea-like plant Photinia rubra[D]. Taian: Shandong Agricultural University, 2012. (in Chinese with English abstract)
[36]	SONG J, LIU J, WENG M L, et al. Cloning of galactinol synthase gene from Ammopiptanthus mongolicus and its expression in transgenic Photinia serrulata plants[J]. Gene, 2013, 513(1): 118-127.
[37]	王海波, 邹竹荣, 龚明. 小桐子肌醇半乳糖苷合成酶3的基因克隆及其生物信息学分析和功能初步验证[J]. 生物技术通报, 2015, 31(7): 91-100.
	WANG H B, ZOU Z R, GONG M. Gene cloning, bioinformatic analysis and preliminary functional characterization of gene encoding galactinol synthase 3 in Jatropha curcas[J]. Biotechnology Bulletin, 2015, 31(7): 91-100. (in Chinese with English abstract)
[38]	贺飞燕, 徐建飞, 简银巧, 等. 过表达肌醇半乳糖苷合成酶基因(ScGolS1)提高转基因马铃薯的耐寒性[C]// 马铃薯产业与种业创新(2022)专题资料汇编, 2022:261-262.
[39]	SHIMOSAKA E, OZAWA K. Overexpression of cold-inducible wheat galactinol synthase confers tolerance to chilling stress in transgenic rice[J]. Breeding Science, 2015, 65(5): 363-371.
[40]	ZUTHER E, BÜCHEL K, HUNDERTMARK M, et al. The role of raffinose in the cold acclimation response of Arabidopsis thaliana[J]. FEBS Letters, 2004, 576(1/2): 169-173.
[41]	赵小萌. 棉子糖寡糖提高番茄幼苗高温抗性的作用研究[D]. 沈阳: 沈阳农业大学, 2019.
	ZHAO X M. Effect of raffinose oligosaccharide on improving high temperature resistance of tomato seedlings[D]. Shenyang: Shenyang Agricultural University, 2019. (in Chinese with English abstract)
[42]	靳娟, 刘晶, 杨磊, 等. 酸枣肌醇半乳糖苷合成酶ZjGolS2基因的克隆及表达分析[J]. 分子植物育种, 2021, 19(13): 4327-4333.
	JIN J, LIU J, YANG L, et al. Cloning and expression analysis of ZjGolS2 in wild jujube(Ziziphus jujuba Mill.var.Spinosa)[J]. Molecular Plant Breeding, 2021, 19(13): 4327-4333. (in Chinese with English abstract)
[43]	沈阳, 贾博为, 王金玉, 等. 拟南芥肌醇半乳糖苷酶AtGolS2基因在非生物胁迫应答中的功能分析[J]. 分子植物育种, 2021, 19(11): 3588-3597.
	SHEN Y, JIA B W, WANG J Y, et al. Functional analysis of Arabidopsis thaliana galactinol synthase AtGolS2 in response to abiotic stress[J]. Molecular Plant Breeding, 2021, 19(11): 3588-3597. (in Chinese with English abstract)
[44]	SUN Z B, QI X Y, WANG Z L, et al. Overexpression of TsGOLS2, a galactinol synthase, in Arabidopsis thaliana enhances tolerance to high salinity and osmotic stresses[J]. Plant Physiology and Biochemistry, 2013, 69: 82-89.
[45]	KONG W L, GONG Z Y, ZHONG H, et al. Expansion and evolutionary patterns of glycosyltransferase family 8 in Gramineae crop genomes and their expression under salt and cold stresses in Oryza sativa ssp. japonica[J]. Biomolecules, 2019, 9(5): 188.
[46]	LI N, ZHANG Z H, CHEN Z J, et al. Comparative transcriptome analysis of two contrasting Chinese cabbage (Brassica rapa L.) genotypes reveals that ion homeostasis is a crucial biological pathway involved in the rapid adaptive response to salt stress[J]. Frontiers in Plant Science, 2021, 12: 683891.
[47]	MARTINS C P S, FERNANDES D, GUIMARÃES V M, et al. Comprehensive analysis of the GALACTINOL SYNTHASE (GolS) gene family in citrus and the function of CsGolS 6 in stress tolerance[J]. PLoS One, 2022, 17(9): e0274791.
[48]	WANG Y G, LIU H H, WANG S P, et al. Overexpression of a common wheat gene GALACTINOL SYNTHASE3 enhances tolerance to zinc in Arabidopsis and rice through the modulation of reactive oxygen species production[J]. Plant Molecular Biology Reporter, 2016, 34(4): 794-806.
[49]	SALVI P, KAMBLE N U, MAJEE M. Stress-inducible galactinol synthase of chickpea (CaGolS) is implicated in heat and oxidative stress tolerance through reducing stress-induced excessive reactive oxygen species accumulation free[J]. Plant and Cell Physiology, 2018, 59(1): 155-166.
[50]	VINSON C C, MOTA A P Z, PORTO B N, et al. Characterization of raffinose metabolism genes uncovers a wild Arachis galactinol synthase conferring tolerance to abiotic stresses[J]. Scientific Reports, 2020, 10(1): 15258.
[51]	KIM M S, CHO S M, KANG E Y, et al. Galactinol is a signaling component of the induced systemic resistance caused by Pseudomonas chlororaphis O6 root colonization[J]. Molecular Plant-Microbe Interactions, 2008, 21(12): 1643-1653.
[52]	WANG D H, LIU Z X, QIN Y, et al. Mulberry MnGolS2 mediates resistance to Botrytis cinerea on transgenic plants[J]. Genes, 2023, 14(10): 1912.
[53]	PHILIPPE R N, RALPH S G, MANSFIELD S D, et al. Transcriptome profiles of hybrid poplar (Populus trichocarpa×deltoides) reveal rapid changes in undamaged, systemic sink leaves after simulated feeding by forest tent caterpillar (Malacosoma disstria)[J]. New Phytologist, 2010, 188(3): 787-802.
[54]	CAO T, LAHIRI I, SINGH V, et al. Metabolic engineering of raffinose-family oligosaccharides in the phloem reveals alterations in carbon partitioning and enhances resistance to green peach aphid[J]. Frontiers in Plant Science, 2013, 4: 263.
[55]	TAKANASHI K, SHITAN N, SUGIYAMA A, et al. Galactinol synthase gene of Coptis japonica is involved in berberine tolerance[J]. Bioscience, Biotechnology, and Biochemistry , 2008, 72(2): 398-405.
[56]	SPRENGER N, KELLER F. Allocation of raffinose family oligosaccharides to transport and storage pools in Ajuga reptans: the roles of two distinct galactinol synthases[J]. The Plant Journal, 2000, 21(3): 249-258.
[57]	HINCHA D K, ZUTHER E, HEYER A G. The preservation of liposomes by raffinose family oligosaccharides during drying is mediated by effects on fusion and lipid phase transitions[J]. Biochimica et Biophysica Acta, 2003, 1612(2): 172-177.
[58]	PANIKULANGARA T J, EGGERS-SCHUMACHER G, WUNDERLICH M, et al. Galactinol synthase1. A novel heat shock factor target gene responsible for heat-induced synthesis of raffinose family oligosaccharides in Arabidopsis[J]. Plant Physiology, 2004, 136(2): 3148-3158.
[59]	WANG Z, ZHU Y, WANG L L, et al. A WRKY transcription factor participates in dehydration tolerance in Boea hygrometrica by binding to the W-box elements of the galactinol synthase (BhGolS1) promoter[J]. Planta, 2009, 230(6): 1155-1166.

Research progress on the function of galactinol synthase gene family in plants

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