Investigation of entomogenous fungi resources in Jiulongshan National Nature Reserve of Suichang County, Zhejiang Province, China

doi:10.3969/j.issn.1004-1524.20241008

Acta Agriculturae Zhejiangensis ›› 2025, Vol. 37 ›› Issue (9): 1891-1904.DOI: 10.3969/j.issn.1004-1524.20241008

• Horticultural Science • Previous Articles Next Articles

Investigation of entomogenous fungi resources in Jiulongshan National Nature Reserve of Suichang County, Zhejiang Province, China

CHEN Yafei¹^,²(), SHI Xiaoxiao², YU Shuisheng³, YE Bihuan², SONG Qiyan², SHEN Jianjun², CHEN Youwu²^,^*()

¹ College of Forestry and Biotechnology, Zhejiang A&F University, Hangzhou 311300, China
² Institute of Forest Protection, Zhejiang Academy of Forestry, Hangzhou 310013, China
³ Zhejiang Jiulongshan National Nature Reserve Management Center, Lishui 323311, Zhejiang, China

Received:2024-11-21 Online:2025-09-25 Published:2025-10-15
Contact: CHEN Youwu

Abstract

Abstract:

To elucidate the entomogenous fungi resources in Jiulongshan National Nature Reserve of Suichang County, Zhejiang Province, a preliminary investigation was conducted in 2024. In this study, a total of 22 specimens were collected. Morphological observations, strain isolation, and purification were performed, followed by molecular identification based on the ribosomal large subunit (LSU) and internal transcribed spacer (ITS) sequences. The results indicated that the isolated entomogenous fungi comprised 11 species across 7 genera, including 5 species of Cordyceps, 1 species of Blackwellomyces, 1 species of Beauveria, 1 species of Tolypocladium, 1 species of Ophiocordyceps, 1 species of Pleurocordyceps, and 1 species of Purpureocillium. Among these, Cordyceps was the dominant genus, with Cordyceps tenuipes being the most prevalent species. The primary hosts of these entomogenous fungi were found to be lepidopteran and hemiptera insects. This study revealed the species and host diversity of entomogenous fungi resources in Jiulongshan National Nature Reserve and provided a foundation for the further development and utilization of these resources in this region.

Key words: entomogenous fungi resources, Jiulongshan National Nature Reserve, Cordyceps tenuipes, lepidopteran insect, host diversity

CLC Number:

S476.12

CHEN Yafei, SHI Xiaoxiao, YU Shuisheng, YE Bihuan, SONG Qiyan, SHEN Jianjun, CHEN Youwu. Investigation of entomogenous fungi resources in Jiulongshan National Nature Reserve of Suichang County, Zhejiang Province, China[J]. Acta Agriculturae Zhejiangensis, 2025, 37(9): 1891-1904.

Figures/Tables 10

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物种 Species	参考序列Reference sequence
物种 Species	LSU	ITS
蜂头线虫草Ophiocordyceps sphecocephala	JN941445	PQ334991
开化布莱克威尔霉Blackwellomyces kaihuaensis	NG₂₄₃₃₀₈	NR₁₉₁₂₆₂
蛹虫草Cordyceps militaris	MT974304	ON024333
独角龙Tolypocladium dujiaolongae	OQ747979	MW242636
侧头虫草属Pleurocordyceps sp.	PP410595	LC669786
细脚虫草/细脚棒束孢Cordyceps tenuipes/Isaria tenuipes	MT974285	GU194179
	MH869511	FJ008675
	OR194074	EF411223
环链虫草/环链拟青霉Cordyceps cateniannulata/Paecilomyces cateniannulatus	MH879589	EU545151
蝉花Cordyceps cicadae	MT555406	MW718303
	MW492907	MW718302
淡紫拟青霉Purpureocillium lilacinum	MH876800	MT529896
粉质虫草/粉质棒束孢Cordyceps farinosa/Isaria farinosa	MH870163	GU354351
球孢白僵菌Beauveria bassiana	MW242686	PP799821

物种 Species	参考序列Reference sequence
物种 Species	LSU	ITS
蜂头线虫草Ophiocordyceps sphecocephala	JN941445	PQ334991
开化布莱克威尔霉Blackwellomyces kaihuaensis	NG₂₄₃₃₀₈	NR₁₉₁₂₆₂
蛹虫草Cordyceps militaris	MT974304	ON024333
独角龙Tolypocladium dujiaolongae	OQ747979	MW242636
侧头虫草属Pleurocordyceps sp.	PP410595	LC669786
细脚虫草/细脚棒束孢Cordyceps tenuipes/Isaria tenuipes	MT974285	GU194179
	MH869511	FJ008675
	OR194074	EF411223
环链虫草/环链拟青霉Cordyceps cateniannulata/Paecilomyces cateniannulatus	MH879589	EU545151
蝉花Cordyceps cicadae	MT555406	MW718303
	MW492907	MW718302
淡紫拟青霉Purpureocillium lilacinum	MH876800	MT529896
粉质虫草/粉质棒束孢Cordyceps farinosa/Isaria farinosa	MH870163	GU354351
球孢白僵菌Beauveria bassiana	MW242686	PP799821

属 Genus	种 Species	菌株编号 Strain number	标本数 Number of specimens
虫草属Cordyceps	细脚虫草C. tenuipes	SC-01、SC-02、SC-05、SC-06、SC-07、SC-08、SC-09、SC-13	8
	环链虫草C. cateniannulata	SC-10	1
	蛹虫草C. militaris	SC-14	1
	蝉花C. cicadae	SC-03、SC-04、SC-20、SC-21、SC-22	5
	粉质虫草C. farinosa	SC-19	1
布莱克威尔霉属Blackwellomyces	开化布莱克威尔霉B. kaihuaensis	SC-12	1
白僵菌属Beauveria	球孢白僵菌B. bassiana	SC-17	1
弯颈霉属Tolypocladium	独角龙T. dujiaolongae	SC-15	1
线虫草属Ophiocordyceps	蜂头线虫草O. sphecocephala	SC-11	1
紫孢霉属Purpureocillium	淡紫拟青霉P. lilacinum	SC-18	1
侧头虫草属Pleurocordyceps	未知Unknown	SC-16	1

属 Genus	种 Species	菌株编号 Strain number	标本数 Number of specimens
虫草属Cordyceps	细脚虫草C. tenuipes	SC-01、SC-02、SC-05、SC-06、SC-07、SC-08、SC-09、SC-13	8
	环链虫草C. cateniannulata	SC-10	1
	蛹虫草C. militaris	SC-14	1
	蝉花C. cicadae	SC-03、SC-04、SC-20、SC-21、SC-22	5
	粉质虫草C. farinosa	SC-19	1
布莱克威尔霉属Blackwellomyces	开化布莱克威尔霉B. kaihuaensis	SC-12	1
白僵菌属Beauveria	球孢白僵菌B. bassiana	SC-17	1
弯颈霉属Tolypocladium	独角龙T. dujiaolongae	SC-15	1
线虫草属Ophiocordyceps	蜂头线虫草O. sphecocephala	SC-11	1
紫孢霉属Purpureocillium	淡紫拟青霉P. lilacinum	SC-18	1
侧头虫草属Pleurocordyceps	未知Unknown	SC-16	1

寄主 Host	各物种的寄生情况Parasitic conditions of each species
寄主 Host	细脚虫草 Cordyceps tenuipes	蝉花 C. cicadae	蜂头线虫草 Ophiocordyceps sphecocephala	蛹虫草 C. militaris	环链虫草 C. cateniannulata	球孢白僵菌 Beauweria bassiana	侧头虫草属 Pleuroco- rdyceps sp.	开化布莱克威尔霉 Blackuellomyces kaihuaensis	独角龙 Tolypocladium dujiaolongae	粉质虫草 C. farinosa	淡紫拟青霉 Purpure- ocillium lilacinum
鳞翅目蛹	√	—	—	√	—	—	—	—	—	—	—
Lepidoptera pupae
鳞翅目幼虫	—	—	—	—	√	—	—	√	—	—	—
Lepidoptera larva
叩头甲	—	—	—	—	—	√	—	—	—	—	—
Elateridae
蜂科Apoidea	—	—	√	—	—	—	—	—	—	—	—
蝉若虫	—	√	—	—	—	—	√	—	√	√	√
Cicadidae nymph

Investigation of entomogenous fungi resources in Jiulongshan National Nature Reserve of Suichang County, Zhejiang Province, China

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调查对象 Subjects of survey	辛普森多样性指数 Simpson’s diversity index(D)	香农-威纳指数 Shannon-Wiener index(H')	物种均匀度指数 Species evenness index(E)
真菌群落Fungal community	0.80	1.96	0.64
寄主Host	0.58	0.99	0.72

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