›› 2010, Vol. 22 ›› Issue (4): 0-432.

• 论文 •    

Tissue culture and rapid micro-propagation from leaves and stems of kiwifruit (Actinidia chinensis cv. Hongyang)

LONG Qian-jin;;WU Yan-jun;XIE Ming;*   

  1. 1College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, China; 2 Institute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-07-25 Published:2010-07-25

Abstract: A stable and efficient rapid propagation system was established from the female Actinidia chinensis cv.Hongyang young leaves and stem segments with axillary buds in vitro. Leaves were cultured for the initiating of callus, proliferation of adventitious buds; and axillary buds were cultured for sprouting, multiplication and rooting. The results indicated that callus formation was easy and proliferation efficiency achieved 100% when explants from leaves were cultured on MS supplemented with 1.0 mg·L-1 zeatin and 0.1 mg·L-1 naphthylacetic acid (NAA). The average multiplication coefficient was 5.2,and the highest induction rate was 93.33%. The medium of MS supplemented with 1.0 mg·L-1 6-benzylaminopurine (6-BA) and 0.1 mg·L-1 NAA was optimal for sprouting of axillary bud,and the highest induction rate was 8333%. The multiplication coefficient of adventitious bud was 4.5 in the medium of MS supplemented with 2.0 mg·L-1 6\|BA, 0.2 mg·L-1 NAA and 0.1 mg·L-1 gibberellic acid (GA). Moreover, the medium of 1/2 MS with 0.7 mg·L-1 indolebutyric acid (IBA) was good for rooting.

Key words: Actinidia chinensis cv.Hongyang, callus, adventitious bud, rooting