Study on the anti-tumor activity of Sanghuangporus spp. fruiting body cultivated with mulberry substrate bag

doi:10.3969/j.issn.1004-1524.20241027

Acta Agriculturae Zhejiangensis ›› 2026, Vol. 38 ›› Issue (3): 481-490.DOI: 10.3969/j.issn.1004-1524.20241027

• Horticultural Science • Previous Articles Next Articles

Study on the anti-tumor activity of Sanghuangporus spp. fruiting body cultivated with mulberry substrate bag

LYU Zhenghua¹(), ZHONG Shi², ZHAO Hui², DONG Xintian², FENG Jiayi², SUN Yuqing², HUO Jinxi², LI Yougui², CHEN Tianfei³^,^*()

^1. Dongyang Dayuan Peach Professional Cooperative, Dongyang 322111, Zhejiang, China
^2. Institute of Sericulture and Tea, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
^3. Chun’an County Agricultural and Rural Development Service Center, Chun’an 311700, Zhejiang, China

Received:2024-11-29 Online:2026-03-25 Published:2026-04-17
Contact: CHEN Tianfei

Abstract

Abstract:

Sanghuangporus spp. have good anti-tumor effects, but the inhibitory effects and functional components of artificially cultivated Sanghuangporus spp. on different tumor cells are still unclear. In this study, the inhibitory effects of water extracts, crude polysaccharide and crude polyphenol of Sanghuangporus spp. on lung cancer cell H1299, liver cancer cell HepG2, gastric cancer cell HGC-27, colon cancer cell HCT116, cervical cancer cell HeLa, prostate cancer cell PC3-1, melanoma cell B16F10, and pancreatic cancer cell Panc1 were evaluated using Sanghuangporus spp. fruit body cultivated in mulberry substrate bags. The result showed that there existed significant differences in the inhibitory effects of water extracts, crude polysaccharides, and crude polyphenols on the proliferation of different tumor cells. The water extracts (800 mg·L^-1) had good inhibitory effects on tumor cells B16F10 (96.57%), HepG2 (96.41%) and HGC-27 (93.93%). Crude polysaccharides (800 mg·L^-1) had high inhibitory activity on tumor cells H1299 (80.95%), HepG2 (80.45%), HGC-27 (85.20%) and B16F10 (81.28%). Crude polyphenols (800 mg·L^-1) had strong inhibitory effects on tumor cells HepG2 (95.11%), HGC-27 (93.41%) and B16F10 (94.94%). The above results indicated that the anti-tumor activity of different components of artificially cultivated Sanghuangporus spp. fruit bodies had cell selectivity, and crude polysaccharides and polyphenols had synergistic activity in inhibiting tumor cell proliferation. Flow cytometry analysis revealed that water extracts could induce S phase arrest in HGC-27 cells, but had no significant effects on cell cycle distribution. Further analysis revealed that water extract (300, 400 mg·L^-1) significantly promoted late stage apoptosis in HGC-27 cells. qRT-PCR analysis revealed that treatment with water extract significantly reduced the expression of anti-apoptotic genes Bcl-2 and Bcl XL in HGC-27 cells, while significantly up-regulating the expression of pro-apoptotic genes Bak, Bid, tBid, and Bax, thereby activating the expression of downstream genes CYCS, Caspase-9, and Caspase-3, thereby promoting cell apoptosis. The results of this study clarified the anti-tumor effect of artificially cultivated Sanghuangporus spp. fruit body, providing scientific basis for promoting the development of Sanghuangporus spp. artificial cultivation industry.

Key words: Sanghuangporus spp., fruiting body, water extracts, crude polysaccharide, crude polyphenol, anti-tumor

CLC Number:

LYU Zhenghua, ZHONG Shi, ZHAO Hui, DONG Xintian, FENG Jiayi, SUN Yuqing, HUO Jinxi, LI Yougui, CHEN Tianfei. Study on the anti-tumor activity of Sanghuangporus spp. fruiting body cultivated with mulberry substrate bag[J]. Acta Agriculturae Zhejiangensis, 2026, 38(3): 481-490.

Figures/Tables 7

Table 1 Primers for qRT-PCR

基因Gene	正向引物序列Forward primer sequence (5'→3')	反向引物序列Reverse primer sequence (5'→3')
Fas	GATGTCAGTCACTTGGGCATT	ATCTGGACCCTCCTACCTCTG
FADD	CCGATGTCATGGAACTCAGACG	CTGGGCTACCTTCCTGGAGAGA
TNFR1	CTGGAGCTGTTGGTGGGAAT	TTGTGGCACTTGGTACAGCA
Bcl-2	GGTGGGGTCATGTGTGTGG	CGGTTCAGGTACTCAGTCATCC
Bcl-xL	TGGGAGGGTAGAGTGGATGGT	GCTTTGAACAGGATACTTTTGTGG
Bak	TACCCTTGTGAGAGCCCAT	GGGAACATAGACCCACCAA
Bid	AGGAAGCCAAACACCAGTAGG	TGTGAACCAGGAGTGAGTCGG
tBid	GGAGCTCAGGGCTGACAAAG	GGGGTCATTCAGCCACTCAA
Bax	CCCGAGAGGTCTTTTTCCGAG	CCAGCCCATGATGGTTCTGAT
CYCS	GCAGGACATGGGCCAAGAAGA	TATCCACAGAAAGCTCAGCAAACCA
Caspase-3	ACACAGCCACAGGTATGAGC	ATCCCGGGTTGACAATGTGG
Caspase-9	GGAATGGATTCTGGGGTCGG	TCGACAACTTTGCTGCTTGC
Caspase-12	TCTCACAGCTCAGATGGTGC	TTACCTTGCAAGAGCCGACC

Fig.1 Effects of different components of artificially cultivated Sanghuangporus spp. fruit bodies treated for 48 hours on the inhibition rates of different tumor cells

Fig.2 Inhibitive effect of different polyphenol monomer compounds from Sanghuangporus spp. on the different cancer cells

Fig.3 Cell cycle analysis of HGC-27 cells exposed to water extract from Sanghuangporus spp. for 48 h SHW, Water extract from Sanghuangporus spp.; * and *** represent significant (p<0.05) and extremely significant (p<0.001) differences, respectively. The same applies to Figures 5 to 6.

Fig.4 Detection results of flow cytometry analyzer B1, Necrosis cell; B2, Late apoptosis cell; B3, Normal cell; B4, Early apoptosis cell.

Fig.5 Apoptosis induced by aqueous extract from Sanghuangporus spp. on HGC-27 cells

Fig.6 Expression of apoptosis associated genes in HGC-27 cells treated with aqueous extract from artificially cultivated Sanghuangporus spp. fruiting bodies

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Study on the anti-tumor activity of Sanghuangporus spp. fruiting body cultivated with mulberry substrate bag

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