Research progress on metabolism and regulation technologies of pear fruit during low-temperature storage

doi:10.3969/j.issn.1004-1524.20250237

Acta Agriculturae Zhejiangensis ›› 2026, Vol. 38 ›› Issue (4): 824-836.DOI: 10.3969/j.issn.1004-1524.20250237

Research progress on metabolism and regulation technologies of pear fruit during low-temperature storage

LI Wenjue¹^,²(), DU Chenfei¹^,², WEI Chunyan², CAI Danying², WANG Yuezhi², SHI Zebin², DAI Meisong²^,^*(), XU Kai¹^,^*()

¹ College of Horticultural Science, Zhejiang A&F University, Hangzhou 311300, China
² Institute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2025-03-24 Online:2026-04-25 Published:2026-05-08

Abstract

Abstract:

Postharvest pears are prone to quality deterioration due to metabolic imbalances. Low-temperature storage is a crucial technique for delaying fruit senescence and maintaining quality. In recent years, metabolomics has been extensively used to reveal the metabolic changes in different pear varieties under low-temperature storage. However, inappropriate low temperatures or prolonged storage can induce cold stress. The molecular mechanisms involve the cascade regulation of cold-responsive genes by transcription factors in the low-temperature signal transduction pathway, subsequently triggering responses in phenylpropanoid metabolism and the reactive oxygen species scavenging system. These processes lead to membrane lipid peroxidation, energy metabolism imbalance, and abnormal accumulation of secondary metabolites, ultimately resulting in typical quality deterioration such as browning, lignification, and aroma loss. Therefore, low-temperature storage often requires combination with other preservation technologies to alleviate chilling injury. This article systematically elaborates on the physiological and metabolic dynamics of pear fruit under low-temperature storage and its molecular regulatory network, reviews the recent advances in the application of low-temperature synergistic technologies for chilling injury prevention and control, and prospects future research directions for analyzing quality changes and developing precise regulation technologies based on multi-omics technologies aiming to provide a theoretical basis for optimizing postharvest preservation technologies for pear fruits.

Key words: pear, low-temperature storage, postharvest biology, metabonomics, regulation technology

CLC Number:

S661.2

LI Wenjue, DU Chenfei, WEI Chunyan, CAI Danying, WANG Yuezhi, SHI Zebin, DAI Meisong, XU Kai. Research progress on metabolism and regulation technologies of pear fruit during low-temperature storage[J]. Acta Agriculturae Zhejiangensis, 2026, 38(4): 824-836.

Figures/Tables 1

Fig.1 Schematic diagram of quality deterioration in pear fruits induced by cold storage PLD, Phospholipase D; LOX, Lipoxygenase; MDA, Malondialdehyde; ROS, Reactive oxygen species; ATPase, ATP synthase; NDA, NADH dehydrogenase; VPPase, Vacuoles-inorganic pyrophosphatase; CAT, Catalase; POD, Peroxidase; SOD, Superoxide dismutase; ATP, Adenosine triphosphate; PAL, Phenylalanine ammonia-lyase; PPO, Polyphenol oxidase; C4H, Cinnamate 4-hydroxylase; 4CL, 4-Coumarate: CoA ligase; HCT, Hydroxycinnamoyl transferase; CCR, Cinnamoyl-CoA reductase; CAD, Cinnamyl alcohol dehydrogenase; FAD, Fatty acid desaturase; HPL, Hydroperoxide lyase; ADH, Alcohol dehydrogenase; AAT, Alcohol acyltransferase; PG, Polygalacturonase; PME, Pectin methylesterase; PL, Pectate lyase; β-Gal, β-Galactosidase. Enzymes, genes and metabolites marked in red font or with red backgrounds indicate those induced by low temperature, while those in green are suppressed by low temperature.

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Research progress on metabolism and regulation technologies of pear fruit during low-temperature storage

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