Changes in the quality of frost mulberry leaf under different frost stress periods

doi:10.3969/j.issn.1004-1524.20250449

Acta Agriculturae Zhejiangensis ›› 2026, Vol. 38 ›› Issue (5): 934-943.DOI: 10.3969/j.issn.1004-1524.20250449

• Horticultural Science • Previous Articles Next Articles

Changes in the quality of frost mulberry leaf under different frost stress periods

LI Jiaqi(), HU Kuanlian, SHEN Zijing, SHI Jianguo^*(), HE Yun

College of Modern Agriculture, Yulin University, Yulin 719000, Shaanxi, China

Received:2025-06-19 Online:2026-05-25 Published:2026-06-02

Abstract

Abstract:

To investigate the impact of frost stress on the quality of frost mulberry leaves, this study systematically investigated the dynamic changes of seven key quality indicators (moisture content, fat content, flavonoids content, total phenols content, fiber content, tannin content and protein content) in mulberry leaves under different frost stress periods (6, 12, 18, and 24 d) using eight mulberry varieties. The results showed that most quality indicators (fat and moisture content) peaked under short-term stress (6 d, 5 ℃). Pearson correlation analysis revealed significant (p<0.05) correlations among these indicators. Based on principal component analysis, five key indicators, fat content, total phenols content, flavonoids content, fiber content, and protein content, were proposed. The comprehensive evaluation revealed that Jingbo mulberry achieved the highest score under 6-day (5 ℃) frost stress, followed by Jing mulberry under 24-day (0 ℃) frost stress. Hanza mulberry, Wubao mulberry, and Fengchi 2 exhibited comparable quality under short-term stress, ranking third, fourth and fifth, respectively. This study provides theoretical support for frost-resistant mulberry breeding and the precise harvesting of frost mulberry leaf.

Key words: frost stress, mulberry germplasm resources, quality of mulberry leaf, principal component analysis

CLC Number:

S888

LI Jiaqi, HU Kuanlian, SHEN Zijing, SHI Jianguo, HE Yun. Changes in the quality of frost mulberry leaf under different frost stress periods[J]. Acta Agriculturae Zhejiangensis, 2026, 38(5): 934-943.

Figures/Tables 11

References 27

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主成分 Principal componet	特征值 Eigenvalue	方差贡献率/% Variance contribution rate/%	累计方差贡献率/% Cumulative variance contribution rate/%
1	2.81	35.123	35.123
2	1.383	17.289	52.412
3	1.216	15.197	67.609
4	1.107	13.837	81.446
5	0.606	7.577	89.023

主成分 Principal componet	特征值 Eigenvalue	方差贡献率/% Variance contribution rate/%	累计方差贡献率/% Cumulative variance contribution rate/%
1	2.81	35.123	35.123
2	1.383	17.289	52.412
3	1.216	15.197	67.609
4	1.107	13.837	81.446
5	0.606	7.577	89.023

指标Index	PC1	PC2	PC3	PC4	PC5
单宁含量 Tannins content	-0.876	0.043	-0.089	0.050	0.039
脂肪含量 Fat content	0.824	0.359	0.195	-0.173	0.063
蛋白质含量 Protein content	-0.103	-0.065	-0.113	0.175	0.961
黄酮含量Flavonoid content	-0.032	-0.163	0.913	0.225	-0.125
含水量Moisture content	0.751	-0.191	-0.362	0.311	-0.045
纤维含量Fiber content	-0.058	0.065	0.213	0.917	0.192
总酚含量Total phenolic content	-0.076	0.957	-0.147	0.058	-0.066

指标Index	PC1	PC2	PC3	PC4	PC5
单宁含量 Tannins content	-0.876	0.043	-0.089	0.050	0.039
脂肪含量 Fat content	0.824	0.359	0.195	-0.173	0.063
蛋白质含量 Protein content	-0.103	-0.065	-0.113	0.175	0.961
黄酮含量Flavonoid content	-0.032	-0.163	0.913	0.225	-0.125
含水量Moisture content	0.751	-0.191	-0.362	0.311	-0.045
纤维含量Fiber content	-0.058	0.065	0.213	0.917	0.192
总酚含量Total phenolic content	-0.076	0.957	-0.147	0.058	-0.066

桑树品种 Mulberry variety	胁迫温度/℃ Stress temperature/℃	得分 Score					综合得分 Comprehensive score	排名 Ranking
桑树品种 Mulberry variety	胁迫温度/℃ Stress temperature/℃	PC1	PC2	PC3	PC4	PC5	综合得分 Comprehensive score	排名 Ranking
镜泊桑 Jingbo mulberry	5	1.63	0.16	0.15	1.60	-0.14	0.88	1
荆桑 Jing mulberry	0	0.09	3.60	1.20	0.25	-0.10	0.85	2
寒杂桑 Hanza mulberry	5	2.16	-0.35	0.92	-1.79	1.02	0.79	3
吴堡桑 Wubu mulberry	5	1.82	-0.21	-0.99	2.05	-1.10	0.64	4
丰驰2号 Fengchi 2	5	1.83	0.11	0.13	0.04	-0.76	0.63	5
镜泊桑Jingbo mulberry	0	-0.48	0.66	0.46	1.41	2.38	0.56	6
荆桑Jing mulberry	5	1.63	0.22	0.60	-0.64	-0.67	0.56	7
选792 XU792	5	1.53	-1.01	-0.23	-1.12	1.05	0.36	8
强桑 Qiang mulberry	5	1.16	-0.37	-0.33	0.11	-0.75	0.23	9
强桑Qiang mulberry	0	-0.33	1.20	-0.07	-0.01	0.74	0.17	10
吴堡桑 Wubu mulberry	3	0.40	-1.07	-0.85	1.16	1.06	0.17	11
吴堡桑 Wubu mulberry	0	-0.48	0.33	-0.28	0.75	1.50	0.16	12
荆桑Jing mulberry	3	-0.07	1.24	0.12	0.01	-0.87	0.07	13
镜泊桑Jingbo mulberry	3	-0.55	0.14	-0.08	1.27	0.52	0.07	14
选792 XU792	0	-0.30	0.42	-0.80	0.05	1.19	0.01	15
荆桑Jing mulberry	1	-0.28	-0.02	1.51	0.03	-1.02	-0.01	16
镜泊桑Jingbo mulberry	1	-0.81	-0.86	1.73	1.25	-0.46	-0.05	17
桂桑62 Gui 62	5	0.80	0.06	-0.30	-1.78	-0.48	-0.07	18
寒杂桑Hanza mulberry	0	-0.65	0.76	0.13	-1.15	1.15	-0.10	19
吴堡桑Wubu mulberry	1	-0.70	-1.58	1.55	0.59	0.55	-0.11	20
强桑 Qiang mulberry	3	-0.38	0.62	-0.93	0.16	-0.10	-0.18	21
丰驰2号Fengchi 2	0	-0.83	0.97	-0.09	0.40	-0.94	-0.25	22
桂桑62 Gui 62	0	-0.89	0.44	-0.33	-1.13	0.77	-0.37	23
强桑 Qiang mulberry	1	-0.87	-1.41	1.09	0.04	0.00	-0.38	24
寒杂桑 Hanza mulberry	3	-0.23	-0.91	-0.16	-1.25	0.45	-0.38	25
选792 XU792	3	-0.35	-0.46	-1.57	-0.17	0.47	-0.42	26
选792 XU792	1	-0.74	-1.39	-0.25	0.00	0.55	-0.47	27
丰驰2号Fengchi 2	1	-0.91	-0.63	0.40	0.26	-1.10	-0.51	28
丰驰2号Fengchi 2	3	-0.24	-0.41	-1.85	0.79	-1.42	-0.55	29
寒杂桑 Hanza mulberry	1	-0.79	-0.71	0.71	-1.06	-1.36	-0.66	30
桂桑62 Gui 62	3	-0.77	0.02	-1.31	-1.24	-0.33	-0.73	31
桂桑62 Gui 62	1	-1.41	0.42	-0.30	-0.91	-1.78	-0.91	32

Changes in the quality of frost mulberry leaf under different frost stress periods

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指标 Indicator	单宁含量 Tannin content	脂肪含量 Fat content	蛋白质含量 Protein content	黄酮含量 Flavonoid content	含水量 Moisture content	纤维含量 Fiber content
脂肪含量 Fat content	-0.611^**
蛋白质含量Protein content	0.151	-0.127
黄酮含量 Flavonoid content	-0.027	0.004	-0.138
含水量Moisture content	-0.587^**	0.397^**	0.006	-0.163
纤维含量Fiber content	0.089	-0.083	0.299^**	0.302^**	0.043
总酚含量Total phenolic content	0.101	0.176	-0.063	-0.215^*	-0.126	0.032

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