Abstract: rimary hepatocytes injury models in vitro were induced by t-BHP in Carassius auratus gibel and treated with aqueous extract of Hibiseus sabdarifa L. by different administration orders. The activities of GPT, MDA, SOD and GSH-PX in cell culture supernatants were measured to study on the protective effects of aqueous extract of Hibiseus sabdarifa L. on acute hepatic injury. The results demonstrated the model of acute liver damage was established by exposing to 1 mmol·L-1 t-BHP for 2 h. Aqueous extract of Hibiseus sabdarifa L. could reduce the injury of liver by increasing the activity of glutathione peroxidase (GSH-PX) and superoxide dismutase (SOD), restraining the lipid peroxidation production-malondisldehyde (MDA) and inhibit alanine minotransferase (GPT) release (P＜0.05 or P＜0.01) in supernatant of cultured hepatocytes injured by t\|BHP induction. The results suggested that aqueous extract of Hibiseus sabdarifa L. possessed direct protective effect on primary hepatocyte injury induced by t-BHP and this effect was probably associated with scavenging oxygen free radicals and anti-oxidative activity of CMPS.Meanwhile, the administration orders of aqueous extract of Hibiseus sabdarifa L. and t-BHP influenced the protection. The protective effects of aqueous extract of Hibiseus sabdarifa L. in defense-treatment group (DT) were significantly better than defense group (D) and treatment group (T) from the analysis of biochemical indicators．

Key words: Carassius auratus gibel, Hibiseus sabdarifa L., primary culture, model