大豆5个新发现ERF基因的生物信息学及表达分析

doi:10.3969/j.issn.1004-1524.2016.10.03

浙江农业学报 ›› 2016, Vol. 28 ›› Issue (10): 1644-1649.DOI: 10.3969/j.issn.1004-1524.2016.10.03

大豆5个新发现ERF基因的生物信息学及表达分析

翟莹¹, 张军², 赵艳¹, 高士童¹, 孙婉姝¹, 张闯¹, 任巍巍¹, 王秀文¹, 王玉书¹

1.齐齐哈尔大学生命科学与农林学院,黑龙江齐齐哈尔 161006;
2.黑龙江省兽医科学研究所,黑龙江齐齐哈尔 161005

收稿日期:2016-02-16 出版日期:2016-10-15 发布日期:2016-11-20
作者简介:翟莹(1982—),女,吉林吉林人,讲师,博士,主要从事植物分子育种研究。E-mail:fairy39809079@126.com
基金资助:
国家自然科学基金项目(31301335); 黑龙江省教育厅科学技术研究项目(12541889); 黑龙江省自然科学基金项目(C201458)

Bioinformatics and expression analysis of 5 newfound ERF genes in soybean

ZHAI Ying¹, ZHANG Jun², ZHAO Yan¹, GAO Shi-tong¹, SUN Wan-shu¹, ZHANG Chuang¹, REN Wei-wei¹, WANG Xiu-wen¹, WANG Yu-shu¹

1. College of Life Science and Agro-forestry, Qiqihar University, Qiqihar 161006, China;
2. Heilongjiang Institute of Veterinary Science, Qiqihar 161005, China

Received:2016-02-16 Online:2016-10-15 Published:2016-11-20

摘要/Abstract

摘要： ERF转录因子广泛存在于植物中并且参与植物对生物及非生物胁迫的响应。从NCBI数据库中获得5个新的ERF基因,GmERFa/b/c/d/e。蛋白序列分析显示,5个ERF基因均含有一个保守的AP2/ERF结合域。进化分析表明,GmERFe与GmERF3和GmERF7同源性最高,GmERFb、GmERFc与GmERF5的同源性最高,GmERFd与GmEREBP1的同源性较高,而GmERFa与其他ERF蛋白的同源性均较低。实时荧光定量PCR结果显示,5个基因都主要在大豆的根和叶中表达。逆境处理后的实时荧光定量PCR结果显示,GmERFd/e主要对乙烯信号和低温产生响应,GmERFb主要对干旱产生响应,而GmERFa主要对低温产生响应。

关键词: 大豆, ERF转录因子, 生物信息学分析, 表达分析

Abstract: ERF transcription factors are widespread in plants, which are widely involved in plant response to biotic and abiotic stress. In this study, five novel ERF gene sequences, ERFa/b/c/d/e, were obtained by blast from NCBI data base. Protein sequences analysis showed that the five ERF proteins all contained a typical AP2/ERF binding domain. Phylogenetic analysis indicated that GmERFe was highly homologous to GmERF3 and GmERF7; GmERFb and GmERFc were highly homologous to GmERF5; GmERFd was homologous to GmEREBP1; while GmERFa had lower homology with other ERF proteins. The expression patterns of five ERF genes in soybean tissues and under stress treatments were analyzed by real-time quantitative PCR. The results showed that the five ERF genes expressed highly in root and leaf. GmERFd/e responded mainly to ethylene signal and low temperature, GmERFb responded mainly to drought, and GmERFa responded mainly to low temperature.

Key words: soybean, ERF transcription factor, bioinformatics analysis, expression analysis

中图分类号:

S565.1

翟莹, 张军, 赵艳, 高士童, 孙婉姝, 张闯, 任巍巍, 王秀文, 王玉书. 大豆5个新发现ERF基因的生物信息学及表达分析[J]. 浙江农业学报, 2016, 28(10): 1644-1649.

ZHAI Ying, ZHANG Jun, ZHAO Yan, GAO Shi-tong, SUN Wan-shu, ZHANG Chuang, REN Wei-wei, WANG Xiu-wen, WANG Yu-shu. Bioinformatics and expression analysis of 5 newfound ERF genes in soybean[J]. , 2016, 28(10): 1644-1649.

参考文献

[1] 崔喜艳, 陈众峰, 陈展宇. AP2/ERF转录因子对植物非生物胁迫应答的研究进展[J]. 吉林农业大学学报, 2015, 37(4): 417-423.
CUI X Y, CHEN Z F, CHEN Z Y. Advances of researches on AP2/ERF transcription factor response to plant abiotic stress[J]. Journal of Jilin Agricultural University , 2015, 37(4): 417-423. (in Chinese with English abstract)
[2] UPADHYAY R K, SONI D H, SINGH R, et al. SIERF 36, an EAR-motif-containing ERF gene from tomato, alters stomatal density and modulates photosynthesis and growth[J]. Journal of Experimental Botany , 2013, 64(11): 3237-3247.
[3] 张淑珍, 华彩峰, 董利东, 等. ERF转录因子及在大豆中的研究进展[J]. 大豆科学, 2015, 34(3): 512-517.
ZHANG S Z, HUA C F, DONG L D, et al. ERF transcription factors and their research advancement in soybean[J]. Soybean Science , 2015, 34(3): 512-517. (in Chinese with English abstract)
[4] THIRUGNANASAMBANTHAM K, DURAIRAJ S, SARAVANAN S, et al. Role of ethylene response transcription factor (ERF) and its regulation in response to stress encountered by plants[J]. Plant Molecular Biology Reporter , 2015, 33(3): 347-357.
[5] OHME-TAKAGI M, SHINSHI H. Ethylene-inducible DNA binding proteins that interact with an ethylene-responsive element[J]. Plant Cell , 1995, 7(2): 173-182.
[6] HAO D, OHME-TAKAGI M, SARAI A. Unique mode of GCC box recognition by the DNA-binding domain of ethylene-responsive element binding factor(ERF domain) in plants[J]. Journal of Biological Chemistry , 1998, 273(41): 26857-26861.
[7] PARK J M, PARK C J, LEE S B, et al. Overexpression of the tobacco Tsi1 gene encoding an EREBP /AP2-type transcription factor enhances resistance against pathogen attack and osmotic stressing tobacco[J]. Plant Cell , 2001, 13(5): 1035-1046.
[8] BUTTNER M, SINGH K B. Arabidopsis thaliana ethylene-responsive element binding protein (AtEBP), an ethylene-inducible, GCC box DNA-binding protein interacts with an ocs element binding protein[J]. Proceedings of the National Academy of Sciences of the United States of America , 1997, 94(11): 5961-5966.
[9] XUE G P, LOVERIDGE C W. HvDRF1 is involved in abscisic acid mediated gene regulation in barley and produces two forms of AP2 transcriptional activators, interacting preferably with a CT-rich element[J]. Plant Journal , 2004, 37(3): 326-339.
[10] FUJIMOTO S Y, OHTA M, USUI A, et al. Arabidopsis ethylene-responsive element binding factors act as transcriptional activators or repressors of GCC box-mediated gene expression[J]. Plant Cell , 2000, 12(3): 393-404.
[11] XU Z S, XIA L Q, CHEN M, et al. Isolation and molecular characterization of the Triticum aestivum L. ethylene-responsive factor 1 ( TaERF 1) that increases multiple stress tolerance[J]. Plant Molecular Biology , 2007, 65(6): 719-732.
[12] ZHAI Y, WANG Y, LI Y J, et al. Isolation and molecular characterization of GmERF 7, a soybean ethylene-response factor that increases salt stress tolerance in tobacco[J]. Gene , 2013, 513(1): 174-183.
[13] ZHAI Y, LI J W, LI X W, et al. Isolation and characterization of a novel transcriptional repressor GmERF 6 from soybean[J]. Biologia Plantarum , 2013, 57(1): 26-32.
[14] OHTA M, MATSUI K, HIRATSU K, et al. Repression domains of class II ERF transcriptional repressors share an essential motif for active repression[J]. Plant Cell , 2001, 13(8): 1959-1968.
[15] DONG L D, CHENG Y X, WU J J, et al. Overexpression of GmERF 5, a new member of the soybean EAR motif-containing ERF transcription factor, enhances resistance to Phytophthora sojae in soybean[J]. Journal of Experimental Botany , 2015, 66(9): 2635-2647.
[16] ZHANG G Y, CHEN M, CHEN X P, et al. Phylogeny, gene structures, and expression patterns of the ERF gene family in soybean ( Glycine max L.) [J]. Journal of Experimental Botany , 2008, 59(15): 4095-4107.
[17] ZHANG Y Y, YANG C W, LI Y, et al. SDIR1 is a RING finger E3 ligase that positively regulates stress-responsive abscisic acid signaling in Arabidopsis [J]. Plant Cell , 2007, 19(6): 1912-1929.
[18] TOURNIER B, SANCHEZ-BALLESTA M T, JONES B, et al. New members of the tomato ERF family show specific expression pattern and diverse DNA-binding capacity to the GCC box element[J]. FEBS Letters , 2003, 550(1-3): 149-154.
[19] ZHANG G Y, CHEN M, LI L C et al. Overexpression of the soybean GmERF 3 gene, an AP2/ERF type transcription factor for increased tolerances to salt, drought, and diseases in transgenic tobacco[J]. Journal of Experimental Botany , 2009, 60(13): 3781-3796.
[20] ONATE-SANCHEZ L, SINGH K B. Identification of Arabidopsis ethylene-responsive element binding factors with distinct induction kinetics after pathogen infection[J]. Plant Physiology , 2002, 128(4): 1313-1322.

编辑推荐

Metrics

阅读次数

全文

1659

HTML			PDF

最新录用	在线预览	正式出版	最新录用	在线预览	正式出版
0	0	2	0	0	1657

来源	本网站	其他网站

次数	1054	605
比例	64%	36%

摘要

540

最新录用	在线预览	正式出版

0	0	540

	来源	本网站

	次数	540
	比例	100%

大豆5个新发现ERF基因的生物信息学及表达分析

Bioinformatics and expression analysis of 5 newfound ERF genes in soybean

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	赵秀平, 王双, 闫星伊, 段强, 张帅, 陈永胜, 李国瑞. 稻瘟病菌MGG-01005的表达纯化与生物信息学分析[J]. 浙江农业学报, 2021, 33(3): 470-478.
[2]	何佳琦, 翟莹, 张军, 邱爽, 李铭杨, 赵艳, 张梅娟, 马天意. 大豆转录因子GmDof1.5的克隆与非生物胁迫诱导表达[J]. 浙江农业学报, 2021, 33(1): 1-7.
[3]	杨乙未, 肖华, 陈浒, 肖聶佳, 郭城. 喀斯特地区不同玫瑰混农林模式的土壤螨类群落结构特征[J]. 浙江农业学报, 2021, 33(1): 112-121.
[4]	王伟科, 陆娜, 闫静, 宋吉玲, 袁卫东, 周祖法. 秀珍菇S-腺苷甲硫氨酸合成酶基因(PpSAMS)的克隆与表达分析[J]. 浙江农业学报, 2021, 33(1): 62-68.
[5]	梁丽琴, 杨瑞, 郜刚. 马铃薯StUOXs基因家族的生物信息学分析[J]. 浙江农业学报, 2020, 32(9): 1523-1532.
[6]	吴佳, 陈朗, 姜涛, 黄国明, 李倬, 李耀东, 张丽, 刘丽霞. 奶牛CSF3基因遗传多态性筛查及其生物信息学分析[J]. 浙江农业学报, 2020, 32(6): 986-993.
[7]	张古文, 沈立, 郑华章, 刘娜, 冯志娟, 龚亚明. 锌指蛋白转录因子Di19参与调控大豆干旱响应的研究进展[J]. 浙江农业学报, 2020, 32(2): 373-382.
[8]	王伟科, 宋吉玲, 陆娜, 袁卫东, 闫静, 陈观平. 秀珍菇原基形成相关基因PpFBD1的克隆与表达研究[J]. 浙江农业学报, 2020, 32(1): 93-97.
[9]	蒋丰千, 李旸, 余大为, 孙敏, 张恩宝. 基于Caffe卷积神经网络的大豆病害检测系统[J]. 浙江农业学报, 2019, 31(7): 1177-1183.
[10]	刘正奎, 吴瑗, 陈琳, 王磊, 牟泓烨, 祝徐航, 王晓杜. 猪流行性腹泻病毒Nsp5基因的原核表达及生物信息学分析[J]. 浙江农业学报, 2019, 31(4): 532-538.
[11]	彭成璐, 张瑜, 丁雪东, 李玉, 冯士彬, 王希春, 李锦春, 吴金节. 11S通过NF-κB信号通路引起猪小肠上皮细胞损伤[J]. 浙江农业学报, 2019, 31(3): 384-391.
[12]	彭成璐, 张瑜, 夏晓冬, 贺濛初, 舒迎霜, 冯士彬, 李玉, 王希春, 李锦春, 吴金节. β-伴大豆球蛋白通过p38/JNK MAPK信号通路引起IPEC-J2细胞损伤[J]. 浙江农业学报, 2019, 31(2): 242-249.
[13]	董新星, 李明丽, 崔艺佳, 兰国湘, 王孝义, 严达伟. 撒坝猪MCUR1基因克隆、生物信息学分析及其组织表达量检测[J]. 浙江农业学报, 2019, 31(11): 1825-1833.
[14]	刘鹏, 金诚谦, 印祥, 宁新杰, 李庆伦. 大豆联合收获机清选装置与关键技术研究进展[J]. 浙江农业学报, 2019, 31(10): 1758-1766.
[15]	王玉书, 王欢, 郭宇, 周明慧, 陈璐, 陈阳. 羽衣甘蓝β-胡萝卜素羟化酶基因的克隆及表达分析[J]. 浙江农业学报, 2019, 31(1): 80-85.