二十二份引进菊芋种质资源的叶片性状分析

doi:10.3969/j.issn.1004-1524.2017.07.13

浙江农业学报 ›› 2017, Vol. 29 ›› Issue (7): 1151-1157.DOI: 10.3969/j.issn.1004-1524.2017.07.13

二十二份引进菊芋种质资源的叶片性状分析

赵孟良¹, 钟启文¹, 刘明池², 李莉^{1, *}

1.青海大学农林科学院/青海省蔬菜遗传与生理重点实验室,青海西宁 810016;
2.国家蔬菜工程技术研究中心,北京 100097

收稿日期:2016-12-13 出版日期:2017-07-20 发布日期:2017-07-24
通讯作者: 李莉,E-mail:yyslili@163.com
作者简介:赵孟良(1986—),男,河南柘城人,助理研究员,博士研究生,主要从事蔬菜遗传育种及生理研究。E-mail:8304269@163.com
基金资助:
青海省蔬菜遗传与生理重点实验室项目(Sczdsys-2014-02); 青海大学昆仑学者专项

Leaf traits analysis of 22 Helianthus tuberosus germplasm resources introduced from abroad

ZHAO Mengliang¹, ZHONG Qiwen¹, LIU Mingchi², LI Li^{1, *}

1. Qinghai Academy of Agriculture and Forestry Sciences, Qinghai University, Qinghai Key Laboratory of Vegetable Genetics and Physiology, Xining 810016, China;
2. National Engineering Research Center for Vegetables, Beijing 100097, China

Received:2016-12-13 Online:2017-07-20 Published:2017-07-24

摘要/Abstract

摘要： 采用原子吸收分光光度法、硫酸与重铬酸钾氧化法及紫外分光光度法,分别对24份菊芋资源叶片的矿质元素、纤维素、绿原酸和黄酮含量进行了测定,并对国外引进的菊芋种源叶片性状进行了鉴定评价。结果表明:22份国外菊芋种源叶片中含水量最高为F12,达27.58%,最低为F16,达19.02%;来自丹麦与法国的菊芋种源叶片含水量均值差异不大,但均低于青芋3号和青芋4号;叶片形状中近圆形3份,长卵圆形1份,卵圆形18份;叶片中K含量最高的为F19,达30.62 mg·g^-1,是青芋3号的2.5倍;Mg含量最高的为D8,达14.17 mg·g^-1,与青芋3号含量差异不大;Fe含量差别不大,范围为0.09~0.19 mg·g^-1;Ca含量最高的为D8,达26.87 mg·g^-1;绿原酸和黄酮含量最高的均为F7,分别达2.55%和1.24 g·100g^-1;粗纤维含量最高的为F9,达16.7%,最低的为F19,达7.36%。通过主成分及聚类分析可知:在遗传距离为0.65处,可将24份菊芋种源划分为3个组;其中第1组叶片主要是卵圆形和近圆形,第2组叶片为长卵圆形,第3组叶片均为卵圆形。不同菊芋种源叶片中各指标间存在差异,综合参试的各项指标,最终筛选出了F19、D8、F9、F7、D14、D11 等6份特异菊芋种源,供今后进一步的研究。

关键词: 菊芋, 种质资源, 叶片性状, 矿质元素, 纤维素, 聚类分析

Abstract: Atomic absorption spectrophotometry, sulfuric acid and potassium dichromate oxidation method and ultraviolet spectrophotometry were used to determine mineral elements, fiber, content of chlorogenic acid and flavonoids in leaves of 24 Helianthus tuberosus resources, and characteristics of leaves which introduced from abroad were evaluated. The results showed that the highest leaf moisture content in 22 species was F12 (27.58%), and the lowest was F16 (19.02%). The mean moisture content in leaves were little difference between the species from Denmark and France, but they were both lower than Qingyu No. 3 and Qingyu No.4. There were 3 subrotund, 1 long ovoid, 18 ovoid in the shape of the leaves. The highest K content in the leaves was F19 (30.62 mg·g^-1), which was 1.5 times higher than Qingyu No.3. The highest Mg content was D8 (14.17 mg·g^-1). Fe content in the all 24 resources had little difference, ranging from 0.09 mg·g^-1 to 0.19 mg·g^-1. The highest Ca content in the leaves was D8 (26.87 mg·g^-1). The highest levels of chlorogenic acid and flavonoids in the leaves were F7, was 2.55% and 1.24g·100g^-1, respectively. The highest fiber content was F9 (16.7%), and the lowest was F19 (7.36%). Through the analysis of the main component and the clustering analysis, when the genetic distance was 0.65, these 24 resources could be divided into three major categories. Leaves shapes in group 1 were ovoid or subrotund, in group 2 were long ovoid, and in group 3 were ovoid. There were differences among the various leaf indexes of different Helianthus tuberosus resources. Finally, F19, D8, F9, F7, D14 and D11 resources were screened out for further study based on their leaf indexes.

Key words: Helianthus tuberosus Linn., germplasm resources, leaf trait, mineral element, cellulose, clustering analysis

中图分类号:

S632.9

赵孟良, 钟启文, 刘明池, 李莉. 二十二份引进菊芋种质资源的叶片性状分析[J]. 浙江农业学报, 2017, 29(7): 1151-1157.

ZHAO Mengliang, ZHONG Qiwen, LIU Mingchi, LI Li. Leaf traits analysis of 22 Helianthus tuberosus germplasm resources introduced from abroad[J]. , 2017, 29(7): 1151-1157.

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二十二份引进菊芋种质资源的叶片性状分析

Leaf traits analysis of 22 Helianthus tuberosus germplasm resources introduced from abroad

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