外源褪黑素对盐胁迫下猕猴桃幼苗酚类物质含量和抗氧化能力的影响

doi:10.3969/j.issn.1004-1524.2017.07.12

浙江农业学报 ›› 2017, Vol. 29 ›› Issue (7): 1144-1150.DOI: 10.3969/j.issn.1004-1524.2017.07.12

外源褪黑素对盐胁迫下猕猴桃幼苗酚类物质含量和抗氧化能力的影响

高帆¹, 夏惠², 袁雪侦¹, 黄守义¹, 刘继³, 梁东^{2, *}

1.四川农业大学园艺学院,四川成都 611130;
2.四川农业大学果蔬研究所,四川成都 611130;
3.成都市农林科学院,四川成都 611130

收稿日期:2017-03-03 出版日期:2017-07-20 发布日期:2017-07-24
通讯作者: 梁东,E-mail:liangeast@sina.com
作者简介:高帆(1993—),女,河南安阳人,硕士研究生,主要从事果树生物技术研究。E-mail:18227551150@163.com
基金资助:
2016年学科建设双支计划; 2016年四川农业大学科研兴趣培养项目(ky2016115)

Effects of exogenous melatonin on phenolic substance content and antioxidant ability of kiwifruit seedlings under salt stress

GAO Fan¹, XIA Hui², YUAN Xuezhen¹, HUANG Shouyi¹, LIU Ji³, LIANG Dong^{2, *}

1. College of Horticulture, Sichuan Agricultural University, Chengdu 611130, China;
2. Institute of Pomology and Olericulture, Sichuan Agricultural University, Chengdu 611130, China;
3. Chengdu Academy of Agriculture and Forestry Sciences, Chengdu 611130, China

Received:2017-03-03 Online:2017-07-20 Published:2017-07-24

摘要/Abstract

摘要： 以当年生野生美味猕猴桃为材料,采用根灌外源褪黑素(melatonin,MT)的方式处理猕猴桃幼苗,探究外源褪黑素对盐胁迫下猕猴桃幼苗相对电导率、酚类物质(总酚、总黄酮、总黄烷醇)含量以及抗氧化能力(DPPH、ABTS、FRAP)的影响。结果表明,外源MT预处理可有效缓解猕猴桃幼苗膜脂过氧化程度,降低相对膜透性水平,同时显著增加总酚和总黄酮含量,提高抗氧化能力。说明MT预处理可以提高猕猴桃的抗盐性,缓解猕猴桃幼苗在盐胁迫下所受的部分伤害。

关键词: 猕猴桃, 外源褪黑素, 盐胁迫, 酚类, 抗氧化能力

Abstract: One-year-old wild Actinidia deliciosa seedlings were treated with exogenous melatonin solution in order to investigate the effects of electrolyte leakage, phenolic substances (total phenolics, total flavonoids and total flavanols) and antioxidant activities were evaluated, including ABTS^.+ radical scavenging capacity (ABTS), DPPH radical scavenging capacity (DPPH), and iron ion reducing ability (FRAP).The results showed that the relative electrolytic leakage was reduced, total phenolics and total flavonoids were significantly increased and antioxidant ability was improved. This suggested that MT pretreatment alleviated the damage by salt stress in kiwifruit seedlings, and thus improved the salt resistance.

Key words: kiwifruit, exogenous melatonin, salt stress, phenolic, antioxidant ability

中图分类号:

S663.9

高帆, 夏惠, 袁雪侦, 黄守义, 刘继, 梁东. 外源褪黑素对盐胁迫下猕猴桃幼苗酚类物质含量和抗氧化能力的影响[J]. 浙江农业学报, 2017, 29(7): 1144-1150.

GAO Fan, XIA Hui, YUAN Xuezhen, HUANG Shouyi, LIU Ji, LIANG Dong. Effects of exogenous melatonin on phenolic substance content and antioxidant ability of kiwifruit seedlings under salt stress[J]. , 2017, 29(7): 1144-1150.

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外源褪黑素对盐胁迫下猕猴桃幼苗酚类物质含量和抗氧化能力的影响

Effects of exogenous melatonin on phenolic substance content and antioxidant ability of kiwifruit seedlings under salt stress

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