黄芩甙对新城疫病毒感染鸡胚成纤维细胞Toll样受体2、3、4、7 mRNA表达的影响

doi:10.3969/j.issn.1004-1524.2017.12.05

浙江农业学报 ›› 2017, Vol. 29 ›› Issue (12): 1986-1993.DOI: 10.3969/j.issn.1004-1524.2017.12.05

黄芩甙对新城疫病毒感染鸡胚成纤维细胞Toll样受体2、3、4、7 mRNA表达的影响

许锐光¹, 贾艳², 胡力文¹, 翁嘉华¹, 邓俊良¹, 胡延春^{1, *}

1.四川农业大学动物医学院/动物疫病与人类健康四川省重点实验室,四川成都 611130;
2.嘉兴职业技术学院农业与环境分院,浙江嘉兴 314036

收稿日期:2017-05-22 出版日期:2017-12-20 发布日期:2018-01-08
通讯作者: 胡延春,E-mail:hychun114@163.com
作者简介:许锐光(1993—),女,河北沧州人,硕士研究生,研究方向为动物环境公害性疾病。E-mail:597033704@qq.com
基金资助:
四川省科技支撑项目(2015SZ0201); 教育部创新团队项目(IRT0848); 四川省学术和技术带头人培养基金项目(2014,2016)

Effect of Baicalin on expression of Toll-like receptor 2, 3, 4, 7 in Newcastle disease virus-infected CEFs

XU Ruiguang¹, JIA Yan², HU Liwen¹, WENG Jiahua¹, DENG Junliang¹, HU Yanchun^{1, *}

1. Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China;
2. Agricultural and Environmental Branch, Jiaxing Vocational Technical College, Jiaxing 314036, China

Received:2017-05-22 Online:2017-12-20 Published:2018-01-08

摘要/Abstract

摘要： 旨在探讨黄芩甙对新城疫病毒感染鸡胚成纤维细胞Toll样受体2、3、4、7 mRNA表达的影响。新城疫病毒(NDV)感染鸡胚成纤维细胞,使用MTT法检测黄芩甙对鸡胚成纤维细胞的最大安全浓度,使用荧光定量PCR方法检测不同浓度黄芩甙对染毒细胞Toll样受体2、3、4、7 mRNA在不同时间表达的影响。结果表明,黄芩甙组和病毒对照组细胞TLR2、TLR4和TLR7 mRNA的表达水平起初受到抑制,TLR2和TLR4 mRNA在24 h内呈上升趋势,TLR7 mRNA在16 h内呈上升趋势,TLR3 mRNA的表达水平呈波动性变化。黄芩甙在24 h时能够促进NDV感染细胞TLR2和TLR4 mRNA的表达,且呈剂量依赖性,从而发挥免疫调节作用,具有抗NDV的功能。

关键词: 黄芩甙, 新城疫病毒, Toll样受体, 实时荧光定量PCR

Abstract: The purpose of this study was to investigate the changes of the expression of TLR2, TLR3, TLR4 and TLR7 gene in NDV-infected cells and the effect of Baicalin on them. NDV infection of chicken embryo fibroblasts (CEFs) was performed. Cytotoxicity of Baicalin was tested by the MTT method. Quantitative expressions of TLR2, TLR3, TLR4 and TLR7 gene were analyzed by real-time fluorescence quantitative PCR (RTFQ PCR). The results showed that the expressions of TLR2, TLR4 and TLR7 genes were suppressed by NDV in the early infection stage, while their expression were enhanced with the replication of NDV and the expression of TLR2 and TLR4 gene reached the peak level at 24 h. As well as the expression of TLR7 gene reached the peak level at 16 h. The infection of NDV could change the expression of TLR3 gene, but it was not regular. Besides, Baicalin could increase the expression of TLR2 and TLR4 genes in NDV-infected cells and show the dosage-dependence, which enhanced immune function and possessed anti-NDV activity.

Key words: Baicalin, Newcastle disease virus (NDV), Toll-like receptors (TLRs), real-time fluorescence quantitative PCR (RTFQ PCR)

中图分类号:

S831.7

许锐光, 贾艳, 胡力文, 翁嘉华, 邓俊良, 胡延春. 黄芩甙对新城疫病毒感染鸡胚成纤维细胞Toll样受体2、3、4、7 mRNA表达的影响[J]. 浙江农业学报, 2017, 29(12): 1986-1993.

XU Ruiguang, JIA Yan, HU Liwen, WENG Jiahua, DENG Junliang, HU Yanchun. Effect of Baicalin on expression of Toll-like receptor 2, 3, 4, 7 in Newcastle disease virus-infected CEFs[J]. , 2017, 29(12): 1986-1993.

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黄芩甙对新城疫病毒感染鸡胚成纤维细胞Toll样受体2、3、4、7 mRNA表达的影响

Effect of Baicalin on expression of Toll-like receptor 2, 3, 4, 7 in Newcastle disease virus-infected CEFs

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