杂交鲟源恶臭假单胞菌的分离鉴定及药敏特性研究

doi:10.3969/j.issn.1004-1524.2017.12.04

浙江农业学报 ›› 2017, Vol. 29 ›› Issue (12): 1978-1985.DOI: 10.3969/j.issn.1004-1524.2017.12.04

杂交鲟源恶臭假单胞菌的分离鉴定及药敏特性研究

杨圆圆¹, 杨移斌^{2, *}, 曹海鹏³, 方伟¹, 林汉群¹, 杨先乐³, 艾晓辉²

1.广东省水生动物疫病预防控制中心,广东广州 510222;
2.中国水产科学研究院长江水产研究所,湖北武汉 430223;
3.上海海洋大学,上海 201306

收稿日期:2017-04-11 出版日期:2017-12-20 发布日期:2018-01-08
通讯作者: 杨移斌,E-mail:yangyb1988@126.com
作者简介:杨圆圆(1984—),女,湖南娄底人,助理工程师,主要从事水生动物病害诊断及其防控技术研究。E-mail:ykyyy9@126.com
基金资助:
公益性行业(农业)科研专项(201503108-CC-1,201203085)

Isolation, identification and antibiotic sensitivity of Pseudomonas putida from hybrid sturgeon (Huso dauricus♀×Acipenser schrenckii♂)

YANG Yuanyuan¹, YANG Yibin^{2, *}, CAO Haipeng³, FANG Wei¹, LIN Hanqun¹, YANG Xianle³, AI Xiaohui²

1. Guangdong Provincial Center for Aquatic Animal Disease Prevention and Control, Guangzhou 510222, China;
2. Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China;
3. Shanghai Ocean University, Shanghai 201306, China

Received:2017-04-11 Online:2017-12-20 Published:2018-01-08

摘要/Abstract

摘要： 对患肠炎病杂交鲟进行病原分离、鉴定及药敏试验,为杂交鲟肠炎病的防控提供参考。从患病杂交鲟肝、肾、脾及肠道中分离纯化病原菌,经理化特性测定及16S rRNA 序列分析对其进行鉴定,开展人工感染试验,并利用K-B法进行药敏特性分析。结果显示,分离到的XY4菌株是本次引发杂交鲟病害的致病菌,其对杂交鲟的LD₅₀为1.30×10⁶ cfu·g^-1。XY4菌株理化特性与恶臭假单胞菌一致,16S rRNA序列与恶臭假单胞菌同源性为100%,综合判定XY4菌株为恶臭假单胞菌。XY4菌株对头孢拉定、氟苯尼考及多西环素等9种抗生素高度敏感。养殖时可选用对恶臭假单胞菌敏感药物进行防控。

关键词: 恶臭假单胞菌, 杂交鲟(Huso dauricus♀×, Acipenser schrenckii♂), 分离鉴定, 药敏特性

Abstract: The isolation, identification and antibiotic sensitivity of pathogen from the morbid hybrid sturgeon were conducted to provide a reference for control and prevention disease for hybrid sturgeon. The pathogenic bacteria were isolated and purified from liver, kidney, spleen and intestinal tract of hybrid sturgeon. The biochemical identification and 16S rRNA gene sequence determination and the artificial infection test were used to identify whether the strain XY4 was the pathogen, and the antimicrobial susceptibility test was conducted by K-B method. The results showed that strain XY4 was the pathogens of hybrid sturgeon, the LD₅₀ of the isolate was counted to 1.30×10⁶ cfu·g^-1. According to morphological and biochemical characteristics as well as the result of 16S rRNA gene sequence analysis, the isolated strain XY4 was Pseudomonas putida. Strain XY4 was susceptible to cefadroxil, florfenicol, doxycycline and other 6 kinds of antibiotics. The disease caused by Pseudomonas putida can be prevented by above sensitive drugs in fishery farming.

Key words: Pseudomonas putida, hybrid sturgeon(Huso dauricus♀×, Acipenser schrenckii♂), isolation and identification, antimicrobial susceptibility

中图分类号:

S941.42

杨圆圆, 杨移斌, 曹海鹏, 方伟, 林汉群, 杨先乐, 艾晓辉. 杂交鲟源恶臭假单胞菌的分离鉴定及药敏特性研究[J]. 浙江农业学报, 2017, 29(12): 1978-1985.

YANG Yuanyuan, YANG Yibin, CAO Haipeng, FANG Wei, LIN Hanqun, YANG Xianle, AI Xiaohui. Isolation, identification and antibiotic sensitivity of Pseudomonas putida from hybrid sturgeon (Huso dauricus♀×Acipenser schrenckii♂)[J]. , 2017, 29(12): 1978-1985.

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杂交鲟源恶臭假单胞菌的分离鉴定及药敏特性研究

Isolation, identification and antibiotic sensitivity of Pseudomonas putida from hybrid sturgeon (Huso dauricus♀×Acipenser schrenckii♂)

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