鸭源大肠杆菌Ⅰ型菌毛的鉴定及FimA、FimH基因特征分析

doi:10.3969/j.issn.1004-1524.2017.12.06

浙江农业学报 ›› 2017, Vol. 29 ›› Issue (12): 1994-1999.DOI: 10.3969/j.issn.1004-1524.2017.12.06

鸭源大肠杆菌Ⅰ型菌毛的鉴定及FimA、FimH基因特征分析

黄书伦¹, 郑凯文¹, 胡洋¹, 李章程¹, 程方俊^{1, 2, *}, 宋振辉^{1, 2}, 周作勇^{1, 2}, 刘国林¹, 林春发¹, 林永润¹, 张婷婷¹

1.西南大学动物科学学院,重庆 402460;
2. 重庆市兽医科学工程研究中心,重庆 402460

收稿日期:2017-05-15 出版日期:2017-12-20 发布日期:2018-01-08
通讯作者: 程方俊,E-mail:cfj-xn@163.com
作者简介:黄书伦(1994—),男,福建南安人,动物医学专业本科生。E-mail:572974812@qq.com
基金资助:
重庆市社会事业与民生保障科技创新专项(CSTC2015SHMSZX80020); 重庆市前沿与应用基础研究(cstc2016jcyA0235); 中央高校基本科研业务费(XDJK2017D083)

Identification of type Ⅰ fimbriae of Escherichia coli isolated from ducks and genetic characteristics of their FimA and FimH genes

HUANG Shulun¹, ZHENG Kaiwen¹, HU Yang¹, LI Zhangcheng¹, CHENG Fangjun^{1, 2, *}, SONG Zhenhui^{1, 2}, ZHOU Zuoyong^{1, 2}, LIU Guolin¹, LIN Chunfa¹, LIN Yongrun¹, ZHANG Tingting¹

1.College of Animal Science, Southwest University, Chongqing 402460, China;
2. Veterinary Science Engineering Research Center of Chongqing, Chongqing 402460, China

Received:2017-05-15 Online:2017-12-20 Published:2018-01-08

摘要/Abstract

摘要： 为了解川渝部分地区鸭源大肠埃希菌Ⅰ型菌毛产生情况及分子特征,采用MSHA/MRHA试验、PCR扩增Ⅰ型菌毛基因(FimA、FimH)进行菌毛类型鉴定;通过分析FimA氨基酸序列的亲水性、抗原性、表面可及性来预测抗原位点。MSHA/MRHA试验结果显示,MSHA检测阳性率为44.3%;PCR检测FimA阳性率为63.41%,FimH阳性率为100%。氨基酸序列比对结果显示:FimA氨基酸序列与比对株大肠埃希菌(ECOR61)相比,存在19个差异位点;FimH氨基酸序列与比对株(ECOR45)相比,仅少数菌株出现1~2个氨基酸位点差异,表明该片段高度保守。对FimA氨基酸序列进行抗原位点预测,结果显示,多数菌株抗原表位共同区域分布在84~88、142~146、149~150位氨基酸之间,除上述位点外,部分菌株在101~105、130~134位氨基酸出现抗原表位。

关键词: 大肠埃希菌, Ⅰ, 型菌毛, 氨基酸, 序列比对, 抗原表位

Abstract: To study the generation and molecular characteristics of Escherichia coli typeⅠfimbriae from ducks in parts of Sichuan-Chongqing region, the typeⅠfimbriae was identified by MSHA/MRHA test, and their FimA and FimH genes were identified by PCR amplification; The hydrophilicity, antigenicity, and surface probability of FimA amino acid sequences were analyzed by DNAStar to predict antigenic sites. The MSHA/MRHA test results showed that the positive rate of MSHA was 44.3%. PCR results indicated that the positive rate of FimA and FimH were 63.41% and 100%, respectively. Amino acid sequences of FimA and FimH were deduced and contrasted respectively with comparative Escherichia coli strain ECOR61 and ECOR45. Amino acid sequence alignment showed that there were 19 different positions in FimA amino acid sequences. However, there were 1-2 different positions in FimH amino acid sequences, it was believed that FimH amino acid sequences were highly conserved. The antigenic sites of FimA amino acid sequences were predicted. The results indicated that antigenic epitopes of most strains were distributed in 84-88, 142-146 and 149-150 amino acids. In addition, some other strains' antigenic epitopes appeared at 101-105 and 130-134 amino acids.

Key words: Escherichia coli, typeⅠ, fimbriae, amino acid, sequence alignment, antigenic epitope

中图分类号:

S855.3

黄书伦, 郑凯文, 胡洋, 李章程, 程方俊, 宋振辉, 周作勇, 刘国林, 林春发, 林永润, 张婷婷. 鸭源大肠杆菌Ⅰ型菌毛的鉴定及FimA、FimH基因特征分析[J]. 浙江农业学报, 2017, 29(12): 1994-1999.

HUANG Shulun, ZHENG Kaiwen, HU Yang, LI Zhangcheng, CHENG Fangjun, SONG Zhenhui, ZHOU Zuoyong, LIU Guolin, LIN Chunfa, LIN Yongrun, ZHANG Tingting. Identification of type Ⅰ fimbriae of Escherichia coli isolated from ducks and genetic characteristics of their FimA and FimH genes[J]. , 2017, 29(12): 1994-1999.

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鸭源大肠杆菌Ⅰ型菌毛的鉴定及FimA、FimH基因特征分析

Identification of type Ⅰ fimbriae of Escherichia coli isolated from ducks and genetic characteristics of their FimA and FimH genes

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