甜樱桃PacAMR1基因的克隆及表达分析

doi:10.3969/j.issn.1004-1524.2018.04.12

浙江农业学报 ›› 2018, Vol. 30 ›› Issue (4): 607-613.DOI: 10.3969/j.issn.1004-1524.2018.04.12

甜樱桃PacAMR1基因的克隆及表达分析

李京霞¹, 夏惠^{1, 2}, 吕秀兰^{1, 2}, 王进^{1, 2}, 梁东^{1, 2, *}

1.四川农业大学园艺学院,四川成都 611130;
2.四川农业大学果蔬研究所,四川成都 611130

收稿日期:2017-10-07 出版日期:2018-04-20 发布日期:2018-04-19
通讯作者: 梁东,E-mail:liangeast@sina.com.cn
作者简介:李京霞(1995—),女,河南原阳人,本科生,主要从事果树生物技术研究。E-mail:1136024913@qq.com
基金资助:
四川省教育厅应用基础重点项目(16ZA0024)

Cloning and expression analysis of PacAMR1 gene in sweet cherry

LI Jingxia¹, XIA Hui^{1, 2}, LYU Xiulan^{1, 2}, WANG Jin^{1, 2}, LIANG Dong^{1, 2, *}

1. College of Horticulture, Sichuan Agricultural University, Chengdu 611130, China;
2. Institute of Pomology and Olericulture, Sichuan Agricultural University, Chengdu 611130, China

Received:2017-10-07 Online:2018-04-20 Published:2018-04-19

摘要/Abstract

摘要： 以甜樱桃果实为材料,利用反转录聚合酶链式反应(RT-PCR)技术,获得了参与调控甜樱桃AsA生物合成的关键基因——PacAMR1。序列同源性分析发现,该基因编码的氨基酸序列与其他植物AMR1蛋白具有较高的同源性。实时荧光定量RT-PCR的结果表明,随着果实发育成熟,PacAMR1基因的表达量逐渐上升,而果实中的AsA含量则是在果实发育早期较高。PacAMR1基因的表达量在幼叶中最低,而在老叶中表达量最高;但相反的是,AsA含量在幼叶中最高,而在老叶中最低。这些结果表明,PacAMR1基因的表达可能参与了AsA生物合成的负调控。

关键词: 抗坏血酸, AMR1基因, 调控, 甜樱桃

Abstract: Ascorbic acid (AsA) biosynthesis in plants occurs through a complex network. Regulation about this network is largely uncharacterized. PacAMR1, a gene that regulates pathway on AsA biosynthesis was identified from sweet cherry by reverse transcription-polymerase chain reaction (RT-PCR). The results of homology analysis showed that PacAMR1 protein shares high sequence similarity with that of other plants. Real-time PCR analysis showed that the relative expression level of PacAMR1 increased gradually with the development of fruit, however the AsA content was higher in early phage of fruit development. As leaves aged, PacAMR1transcripts accumulated with a concomitant increase. In contrast, the AsA content was highest in young leaves and lowest in old leaves. These results indicated that PacAMR1 appears to play an important role in modulating AsA biosynthesis in sweet cherry by negative regulating.

Key words: ascorbic acid, AMR1 gene, regulation, sweet cherry

中图分类号:

S662.5

李京霞, 夏惠, 吕秀兰, 王进, 梁东. 甜樱桃PacAMR1基因的克隆及表达分析[J]. 浙江农业学报, 2018, 30(4): 607-613.

LI Jingxia, XIA Hui, LYU Xiulan, WANG Jin, LIANG Dong. Cloning and expression analysis of PacAMR1 gene in sweet cherry[J]. , 2018, 30(4): 607-613.

参考文献

[1] FOYER C H, NOCTOR G.Ascorbate and glutathione: The heart of the redox hub[J]. Plant Physiology , 2011, 155(1):12-18.
[2] LAING WA, BULLEY S, WRIGHT M, et al. A highly specific L-galactose-1-phosphate phosphatase on the path to ascorbate biosynthesis[J]. Proceedings of the National Academy of Sciences of the United States of America , 2004, 101(48):16976-16981.
[3] BULLEY S M, RASSAM M, HOSER D, et al. Gene expression studies in kiwifruit and gene over-expression in Arabidopsis indicates that GDP-L-galactose guanyltransferase is a major control point of vitamin C biosynthesis[J]. Journal of Experimental Botany , 2009, 60(3):765-778.
[4] LI M, MA F, LIANG D, et al. Ascorbate biosynthesis during early fruit development is the main reason for its accumulation in kiwi[J]. PLoS One , 2010, 5(12): e14281.
[5] HUANG M, XU Q, DENG X X. L-Ascorbic acid metabolism during fruit development in an ascorbate-rich fruit crop chestnut rose ( Rosa roxburghii Tratt)[J]. Journal of Plant Physiology , 2014, 171(14):1205-1216.
[6] LI M, CHEN X, WANG P, et al. Ascorbic acid accumulation and expression of genes involved in its biosynthesis and recycling in developing apple fruit[J]. Journal of the American Society for Horticultural Science , 2011, 136(4):231-238.
[7] IOANNIDI E, KALAMAKI M S, ENGINEER C, et al. Expression profiling of ascorbic acid-related genes during tomato fruit development and ripening and in response to stress conditions[J]. Journal of Experimental Botany , 2009, 60(2):663-678.
[8] CRUZ-RUS E, BOTELLA M A, VALPUESTA V, et al. Analysis of genes involved in L-ascorbic acid biosynthesis during growth and ripening of grape berries[J]. Journal of Plant Physiology , 2010, 167(9):739-748.
[9] CRUZ-RUS E, AMAYA I, SÁNCHEZ-SEVILLA J F, et al. Regulation of L-ascorbic acid content in strawberry fruits[J]. Journal of Experimental Botany , 2011, 62(12):4191-4201.
[10] LINSTER C L, CLARKE S G. L-ascorbate biosynthesis in higher plants: the role of VTC2[J]. Trends in Plant Science , 2008, 13(11):567-573.
[11] ALÓS E, RODRIGO M J, ZACARÍAS L. Differential transcriptional regulation of L-ascorbic acid content in peel and pulp of citrus fruits during development and maturation[J]. Planta , 2014, 239(5): 1113-1128.
[12] YE J, HU T X, YANG C M, et al. Transcriptome profiling of tomato fruit development reveals transcription factors associated with ascorbic acid, carotenoid and flavonoid biosynthesis[J]. PLoS One , 2015, 10(7): e0130885.
[13] LIU F H, WANG L, GU L, et al. Higher transcription levels in ascorbic acid biosynthetic and recycling genes were associated with higher ascorbic acid accumulation in blueberry[J]. Food Chemistry , 2015, 188: 399-405.
[14] ZHANG C M, HUANG J, LI X G. Transcriptomic analysis reveals the metabolic mechanism of l-ascorbic acid in Ziziphus jujuba Mill.[J]. Frontiers in Plant Science , 2016, 7: 122.
[15] MOON J, PARRY G, ESTELLE M. The ubiquitin-proteasome pathway and plant development[J]. Plant Cell , 2004, 16(12): 3181-3195.
[16] NI W, XIE D, HOBBIE L, et al. Regulation of flower development in Arabidopsis by SCF complexes[J]. Plant Physiology , 2004, 134(4): 1574-1585.
[17] ZHANG W, LORENCE A, GRUSZEWSKI H A, et al. AMR1, an Arabidopsis gene that coordinately and negatively regulates the mannose/L-galactose ascorbic acid biosynthetic pathway[J]. Plant Physiology , 2009, 150(2):942-950.
[18] GASIC K, HERNANDEZ A, KORBAN S S. RNA extraction from different apple tissues rich in polyphenols and polysaccharides for cDNA library construction[J]. Plant Molecular Biology Reporter , 2004, 22(4):437-438.
[19] BAI C, SEN P, HOFMANN K, et al. SKP1 connects cell cycle regulators to the ubiquitin proteolysis machinery through a novel motif, the F-box[J]. Cell , 1996, 86(2): 263-274.
[20] XIAO W Y, JANG J C. F-box proteins in Arabidopsis[J]. Trends in Plant Science , 2000, 5(11):454-457.
[21] WOO H R, CHUNG K M, PARK J H, et al. ORE9, an F-box protein that regulates leaf senescence in Arabidopsis[J]. Plant Cell , 2001, 13(8): 1779-1790.
[22] LIANG D, ZHU T T, NI Z Y, et al. Ascorbic acid metabolism during sweet cherry ( Prunus avium ) fruit development[J]. PLoS One , 2017, 12(2): e0172818.

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甜樱桃PacAMR1基因的克隆及表达分析

Cloning and expression analysis of PacAMR1 gene in sweet cherry

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