浙江农业学报 ›› 2019, Vol. 31 ›› Issue (8): 1361-1371.DOI: 10.3969/j.issn.1004-1524.2019.08.18

• 环境科学 • 上一篇    下一篇

棉花不同发育时期根际微生物的动态变化

刘珊珊1, 韦鑫1, 盛福瑞1, 乔清华1,2,*   

  1. 1.玉林师范学院 生物与制药学院,广西 玉林 537000;
    2.山东棉花研究中心,农业农村部黄淮海棉花遗传改良与栽培生理重点实验室,山东 济南 250100
  • 收稿日期:2019-03-27 出版日期:2019-08-25 发布日期:2019-08-30
  • 通讯作者: 乔清华,E-mail: 15066499860@163.com
  • 作者简介:刘珊珊(1997-),女,广西贵港人,本科生,研究方向为根际微生物。E-mail: ylsylss095@163.com
  • 基金资助:
    泰山学者建设工程专项(ts201511070); 国家科技重大专项--转基因生物新品种培育(2016ZX08005-003-008); 国家现代农业产业技术体系(CARS-18-10); 玉林师范学院校级科研项目(2019YJKY22)

Variations of cotton rhizosphere microbial community in different growth stages

LIU Shanshan1, WEI Xin1, SHENG Furui1, QIAO Qinghua1,2,*   

  1. 1. College of Biology and Pharmacy, Yulin Normal University, Yulin 537000, China;
    2. Key Laboratory of Cotton Breeding and Cultivation in Huang-Huai-Hai Plain, Ministry of Agriculture and Rural Affairs, Cotton Research Center, Shandong Academy of Agricultural Sciences, Jinan 250100, China
  • Received:2019-03-27 Online:2019-08-25 Published:2019-08-30

摘要: 为了解棉花不同发育时期根际细菌和真菌的群落结构和多样性变化,对2个棉花栽培种--陆地棉和海岛棉不同发育时期的根际土壤及未种植棉花的对照土DNA进行16S和ITS扩增子测序与分析。结果表明:棉花各发育时期根际土中细菌和真菌的物种丰富度显著(P<0.05)小于对照土,根际土中的物种丰富度在蕾期最高。棉花苗期与蕾期根际细菌群落之间的差异菌群进化距离较远。聚类分析表明,变形菌门(Proteobacteria)、酸杆菌门(Acidobacteria)、厚壁菌门(Firmicutes)、蓝细菌门(Cyanobacteria)、放线菌门(Actinobacteria)、浮霉菌门(Planctomycetes)、拟杆菌门(Bacteroidetes)和疣微菌门(Verrucomicrobia)为棉花根际细菌的优势菌门,子囊菌门(Ascomycota)、担子菌门(Basidiomycota)和接合菌门(Zygomycota)为棉花根际真菌的优势菌门。此外,棉花根际细菌和真菌群落中相对丰度较对照显著(P<0.05)升高和降低的菌属在不同发育时期存在差异,其中,在根际土中相对丰度显著(P<0.05)升高的菌属在蕾期最多。

关键词: 棉花, 发育时期, 根际, 细菌, 真菌

Abstract: To understand the cotton rhizosphere bacterial and fungal community structure during different growth stages, 16S and ITS regions of soil DNAs sampled from the rhizosphere and corresponding control soil of upland and island cotton were sequenced and analyzed. The results indicated that species richness of bacteria and fungi in rhizosphere of different growth stages were significantly (P<0.05) lower than that of control soil, and the species richness in rhizosphere were the highest in the budding stage. Evolutionary distance between the microbiome leading to the difference between seedling stage and budding stage was farther than that between budding stage and flowering stage. Cluster analysis showed that dominate phyla of cotton rhizosphere bacterial community were Proteobacteria, Acidobacteria, Firmicutes, Cyanobacteria, Actinobacteria, Planctomycetes, Bacteroidetes and Verrucomicrobia, and dominate phyla of cotton rhizosphere fungal community were Ascomycota, Basidiomycota and Zygomycota. Moreover, the cotton rhizosphere bacterial and fungal genus with higher or lower relative abundance compared with control soil varied in different growth stages, and the quantity of genus with significantly (P<0.05) higher relative abundance was the highest in budding stage.

Key words: cotton, growth stage, rhizosphere, bacteria, fungi

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