缺磷抑制拟南芥对镉的吸收

doi:10.3969/j.issn.1004-1524.2020.05.07

浙江农业学报 ›› 2020, Vol. 32 ›› Issue (5): 804-809.DOI: 10.3969/j.issn.1004-1524.2020.05.07

缺磷抑制拟南芥对镉的吸收

朱森林, 梅忠, 邢承华^*

金华职业技术学院农业与生物工程学院,浙江金华 321004

收稿日期:2019-12-06 出版日期:2020-05-25 发布日期:2020-05-29
通讯作者: *,邢承华,E-mail: xingchenghua@hotmail.com
作者简介:朱森林(1961—),男,浙江义乌人,讲师,主要从事植物生理与食品营养研究。E-mail: zhusenlin667255@163.com
基金资助:
国家自然科学基金 (41571049,41461010); 浙江省公益研究计划(LGN18C140006,LGN19C140009)

Inhibition of phosphorus deficiency on cadmium accumulation in Arabidopsis thaliana

ZHU Senlin, MEI Zhong, XING Chenghua^*

Agriculture & Biological Engineering College, Jinhua Polytechnic, Jinhua 321004, China

Received:2019-12-06 Online:2020-05-25 Published:2020-05-29

摘要/Abstract

摘要： 以野生型拟南芥Col-0和铁转运蛋白缺失突变体irt1为试验材料,设置缺磷无镉处理(-P-Cd)、缺磷加镉处理(-P+Cd)、正常供磷无镉处理(+P-Cd)和正常供磷加镉处理(+P+Cd),研究缺磷对拟南芥镉吸收的影响。结果表明:40 μmol·L^-1 镉处理条件下,与正常供磷相比,缺磷处理显著提高了Col-0拟南芥叶片的叶绿素相对含量和植株干质量,缓解了镉对拟南芥Col-0的毒害;缺磷处理降低了Col-0植株体内的镉含量,并抑制了根中铁转运蛋白基因IRT1的表达;在irt1突变体中,缺磷处理虽然也降低了植株体内的镉含量,但降低幅度显著低于Col-0植株。因此,推测IRT1基因在缺磷抑制拟南芥对镉的吸收、缓解镉毒害中起作用。

关键词: 镉毒害, 缺磷, 拟南芥, 铁转运蛋白

Abstract: Wild-type Arabidopsis thaliana Col-0 and iron transporter deletion mutant irt1 were used as experimental materials to study the mechanism of phosphorus deficiency. The following tests were applied: phosphorus-free plus cadmium-free treatment (-P-Cd), phosphorus-free plus cadmium treatment (-P+Cd), normal phosphorus supply without cadmium treatment (+P-Cd) and normal phosphorus supply plus cadmium treatment (+P+Cd). The results showed that under the condition of 40 μmol·L^-1 cadmium treatment, phosphorus deficiency treatment significantly raised relative leaf chlorophyll content and dry weight of wild-type Arabidopsis thaliana Col-0, and attenuated the toxicity of cadmium to wild-type Arabidopsis thaliana Col-0 compared with normal phosphorus supply. At the same time, phosphorus deficiency treatment reduced the cadmium content in Col-0 plants and inhibited the expression of iron transporter 1 (IRT1) in roots. Phosphorus deficiency treatment in the irt1 mutant also reduced cadmium content in plants, but the reduction was significantly lower than the results obtained for Col-0. Therefore, IRT1 gene played a role in inhibiting cadmium uptake and relieving cadmium toxicity.

Key words: cadmium toxicity, phosphorus deficiency, Arabidopsis thaliana iron transporter 1

中图分类号:

S153
Q945

朱森林, 梅忠, 邢承华. 缺磷抑制拟南芥对镉的吸收[J]. 浙江农业学报, 2020, 32(5): 804-809.

ZHU Senlin, MEI Zhong, XING Chenghua. Inhibition of phosphorus deficiency on cadmium accumulation in Arabidopsis thaliana[J]. , 2020, 32(5): 804-809.

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缺磷抑制拟南芥对镉的吸收

Inhibition of phosphorus deficiency on cadmium accumulation in Arabidopsis thaliana

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