MicroRNA-499对中华鳖脂类代谢相关基因表达的影响

doi:10.3969/j.issn.1004-1524.2020.05.06

浙江农业学报 ›› 2020, Vol. 32 ›› Issue (5): 798-803.DOI: 10.3969/j.issn.1004-1524.2020.05.06

MicroRNA-499对中华鳖脂类代谢相关基因表达的影响

卢祎, 高有领^*, 王水涛, 何盛盛

浙江万里学院生物与环境学院,浙江宁波 315100

收稿日期:2019-11-08 出版日期:2020-05-25 发布日期:2020-05-29
通讯作者: *,高有领,E-mail: gaoyl@zwu.edu.cn
作者简介:卢祎(1994—),女,浙江兰溪人,硕士研究生,研究方向为水产动物营养与饲料科学。E-mail: 1247433485@qq.com
基金资助:
浙江省公益技术应用研究项目(2017C32023)

Effects of microRNA-499 on lipid metabolism-related gene expression in Pelodiscus sinensis

LU Yi, GAO Youling^*, WANG Shuitao, HE Shengsheng

College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, China

Received:2019-11-08 Online:2020-05-25 Published:2020-05-29

摘要/Abstract

摘要： 采用体内注射microRNA-499特异性抑制剂antagomirs的方法,揭示microRNA-499对中华鳖脂类代谢相关基因的调控作用。选取健康稚鳖15只,随机分成3组,分别设为空白对照组、注射生理盐水组和注射miR-499 antagomirs组。在同一时间点连续注射3 d后,测定中华鳖血清和肝脏中甘油三酯和总胆固醇含量,采用实时荧光定量PCR测定肝脏中microRNA-499和脂类代谢相关基因表达水平。结果表明,相对于空白对照组和注射生理盐水组,注射miR-499 antagomirs导致中华鳖肝脏甲状腺激素应答蛋白(THRSP)和钠/碘共同转运体(NIS)的表达水平升高,肉碱棕榈酰转移酶1(CPT1)表达水平降低(P<0.05)。microRNA-499影响中华鳖脂类代谢相关基因的表达水平,这可能与中华鳖脂肪生成和脂肪酸β-氧化有关。

关键词: 中华鳖, miRNA-499, antagomirs, 脂类代谢, 基因表达

Abstract: In this study, a specific inhibitor antagomirs of microRNA-499(miR-499) was injected to reveal the regulatory effect of microRNA-499 on lipid metabolism related genes of Pelodiscus sinensis. Fifteen healthy juvenile Pelodiscus sinensis were selected and randomly divided into control group, saline group and miR-499 antagomirs group, respectively. After 3 days of continuous injection at the same time, triglyceride and total cholesterol in serum and liver were determined, and the expression levels of miR-499 and lipid metabolism related genes in liver were determined by real-time quantitative PCR. The results showed that the injection of miR-499 antagomirs increased the expression levels of thyroid hormone response protein gene(THRSP) and sodium/iodine co-transporter gene(NIS), and decreased the expression level of carnitine palmitoyl transferase 1 gene(CPT1) compared with the control group and the saline group (P<0.05). The injection of miR-499 antagomirs affected the expression level of lipid metabolism genes, and miR-499 was related to fat production and fatty acid β-oxidation of Pelodiscus sinensis.

Key words: Pelodiscus sinensis, miRNA-499, antagomirs, lipid metabolism, gene expression

中图分类号:

S917.4

卢祎, 高有领, 王水涛, 何盛盛. MicroRNA-499对中华鳖脂类代谢相关基因表达的影响[J]. 浙江农业学报, 2020, 32(5): 798-803.

LU Yi, GAO Youling, WANG Shuitao, HE Shengsheng. Effects of microRNA-499 on lipid metabolism-related gene expression in Pelodiscus sinensis[J]. , 2020, 32(5): 798-803.

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MicroRNA-499对中华鳖脂类代谢相关基因表达的影响

Effects of microRNA-499 on lipid metabolism-related gene expression in Pelodiscus sinensis

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