苦荞蔗糖转运体家族FtSUCs的鉴定与生物信息学分析

doi:10.3969/j.issn.1004-1524.2022.08.03

浙江农业学报 ›› 2022, Vol. 34 ›› Issue (8): 1591-1598.DOI: 10.3969/j.issn.1004-1524.2022.08.03

苦荞蔗糖转运体家族FtSUCs的鉴定与生物信息学分析

梁成刚(), 汪燕^*(), 关志秀, 韦春玉, 邓娇, 黄娟, 孟子烨, 石桃雄

贵州师范大学生命科学学院荞麦产业技术研究中心, 贵州贵阳 550001

收稿日期:2021-03-17 出版日期:2022-08-25 发布日期:2022-08-26
通讯作者: 汪燕
作者简介:*汪燕,E-mail: yanwanguf@163.com
梁成刚(1985—),男,重庆人,博士,副教授,研究方向为作物生理及其调控。E-mail: jesselcg@163.com
基金资助:
国家自然科学基金(32060511);国家自然科学基金(31660366);贵州省科技支撑计划(黔科合ZC20192298);贵州师范大学博士启动基金项目;贵州省荞麦种质资源保护与创新重点实验室建设项目(黔教合KY2017002)

Identification and bioinformatics analysis of sucrose transporter family FtSUCs in Tartary buckwheat

LIANG Chenggang(), WANG Yan^*(), GUAN Zhixiu, WEI Chunyu, DENG Jiao, HUANG Juan, MENG Ziye, SHI Taoxiong

Buckwheat Technology Research Center, College of Life Science, Guizhou Normal University,Guiyang 550001, China

Received:2021-03-17 Online:2022-08-25 Published:2022-08-26
Contact: WANG Yan

摘要/Abstract

摘要：

植物蔗糖转运体家族蛋白( sucrose transporter, SUC)是介导蔗糖跨膜运输的重要载体。本文基于苦荞幼苗发育过程中转录组测序获得的数据库,筛选鉴定到10个FtSUCs基因,序列长度在1 436~7 109 bp,编码氨基酸148~605个,其中8个FtSUCs可能定位于内质网,其序列一致性为34.30%,保守位点达到20个。苦荞FtSUCs与拟南芥AtSUCs基因被聚为3类,FtSUCs中4个归入类型Ⅰ,2个归入类型Ⅱ,4个归入类型Ⅲ。苦荞FtSUCs蛋白主要由α-螺旋、不规则卷曲、延长链与β-折叠组成,其中9个FtSUCs包含跨膜结构域。幼苗发育过程中,FtSUCs基因间表达模式存在差异,其中,FtPinG0002608200.01、FtPinG0001943900.01、FtPinG0001944100.01在子叶期高表达,FtPinG0000342600.01表达量逐渐提高,FtPinG0001943500.01和FtPinG0009584000.01在各时期稳定表达,推测它们可能在不同发育阶段发挥特定功能。苦荞中多个FtSUCs基因存在显著正相关或负相关关系,暗示FtSUCs基因间可能存在协同效应或功能互补,进而共同调控蔗糖的跨膜运输。

关键词: 苦荞, 蔗糖转运体基因, 生物信息学, 基因表达模式

Abstract:

Plant sucrose transporter family proteins (SUC) are crucial carriers that mediate sucrose transmembrane transport. In this work, a total of 10 FtSUC genes were screened and identified based on the database of transcriptome sequencing in developing seedling of Tartary buckwheat, of which gene and encoding protein sequence were 1 436-7 109 bp and 148-605 aa, respectively. Eight FtSUCs were predicted to be located in the endoplasmic reticulum, of which sequence consistency was 34.30% including 20 conserved locis. Tartary buckwheat FtSUCs and Arabidopsis AtSUCs were classified into three types, including four FtSUCs in types Ⅰ, two FtSUCs in types Ⅱ and four FtSUCs in types Ⅲ. Tartary buckwheat FTSUCs proteins were mainly composed of α-helix, irregular coil, elongated chain and β-fold. Among them, 9 FtSUCs contain transmembrane domains. The expressing patterns of FtSUCs varied in developing stem of Tartary buckwheat seedlings. For these genes, FtPinG0002608200.01, FtPinG0001943900.01 and FtPinG0001944100.01 were highly expressed at cotyledon stage, while FtPinG0000342600.01 showed gradually increased gene expressing pattern, whereas FtPinG0001943500.01 and FtPinG0009584000.01 exhibited stable gene expressing pattern during seedling development. It was speculated that the varied gene expressing pattern of FtSUCs might perform specific functions at different developmental stages. Correlation analysis confirmed that there were significantly positive or negative correlations among several FtSUCs genes in Tartary buckwheat, suggesting that the synergistic or complementary effects might be occurred among FtSUCs genes, which together regulated the sucrose transmembrane.

Key words: Tartary buckwheat, sucrose transporter gene, bioinformatics, gene expressing pattern

中图分类号:

梁成刚, 汪燕, 关志秀, 韦春玉, 邓娇, 黄娟, 孟子烨, 石桃雄. 苦荞蔗糖转运体家族FtSUCs的鉴定与生物信息学分析[J]. 浙江农业学报, 2022, 34(8): 1591-1598.

LIANG Chenggang, WANG Yan, GUAN Zhixiu, WEI Chunyu, DENG Jiao, HUANG Juan, MENG Ziye, SHI Taoxiong. Identification and bioinformatics analysis of sucrose transporter family FtSUCs in Tartary buckwheat[J]. Acta Agriculturae Zhejiangensis, 2022, 34(8): 1591-1598.

图/表 6

参考文献 21

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基因编号Gene ID	GSL/bp	MTSL/bp	AAL	RMW/u	TIP	AI	PHP	LC
FtPinG0000184200.01	7 109	1 818	605	65 140.75	5.96	97.01	0.318	ER
FtPinG0000812400.01	3 802	549	182	19 782.90	4.32	105.05	0.663	ER
FtPinG0002608200.01	2 259	831	276	30 076.65	8.43	111.63	0.625	ER
FtPinG0009584000.01	1 436	1 038	345	38 316.55	8.7	109.34	0.651	ER
FtPinG0001943900.01	2 486	447	148	16 503.56	11.62	60.54	0.751	CN
FtPinG0008441300.01	3 691	1 263	420	45 212.66	6.60	105.19	0.539	ER
FtPinG0000342600.01	3 387	891	296	32 536.03	9.52	111.72	0.621	ER
FtPinG0001943500.01	2 610	573	190	20 759.12	11.37	79.63	-0.018	CN
FtPinG0005577300.01	2 036	1 527	508	54 119.64	8.83	111.22	0.630	ER
FtPinG0001944100.01	2 849	1 251	416	25 182.57	6.30	104.35	0.468	ER

基因编号Gene ID	GSL/bp	MTSL/bp	AAL	RMW/u	TIP	AI	PHP	LC
FtPinG0000184200.01	7 109	1 818	605	65 140.75	5.96	97.01	0.318	ER
FtPinG0000812400.01	3 802	549	182	19 782.90	4.32	105.05	0.663	ER
FtPinG0002608200.01	2 259	831	276	30 076.65	8.43	111.63	0.625	ER
FtPinG0009584000.01	1 436	1 038	345	38 316.55	8.7	109.34	0.651	ER
FtPinG0001943900.01	2 486	447	148	16 503.56	11.62	60.54	0.751	CN
FtPinG0008441300.01	3 691	1 263	420	45 212.66	6.60	105.19	0.539	ER
FtPinG0000342600.01	3 387	891	296	32 536.03	9.52	111.72	0.621	ER
FtPinG0001943500.01	2 610	573	190	20 759.12	11.37	79.63	-0.018	CN
FtPinG0005577300.01	2 036	1 527	508	54 119.64	8.83	111.22	0.630	ER
FtPinG0001944100.01	2 849	1 251	416	25 182.57	6.30	104.35	0.468	ER

基因编号 Gene ID	α-螺旋 α-helix/%	β-折叠 β-turn/%	延长链 Extended strand/%	无规则卷曲 Random coil/%	跨膜域 Transmembrane domain
FtPinG0000184200.01	37.02	3.64	14.88	44.46	11
FtPinG0000812400.01	48.35	4.40	16.48	30.77	3
FtPinG0002608200.01	29.34	6.16	17.03	47.46	5
FtPinG0009584000.01	54.05	2.60	12.72	30.64	8
FtPinG0001943900.01	14.86	5.41	20.27	59.46	0
FtPinG0008441300.01	50.00	2.86	15.24	31.90	10
FtPinG0000342600.01	50.68	4.39	17.23	27.70	6
FtPinG0001943500.01	32.11	5.26	15.79	46.84	2
FtPinG0005577300.01	46.06	3.94	15.16	34.84	10
FtPinG0001944100.01	53.91	4.35	13.04	28.70	10

基因编号 Gene ID	α-螺旋 α-helix/%	β-折叠 β-turn/%	延长链 Extended strand/%	无规则卷曲 Random coil/%	跨膜域 Transmembrane domain
FtPinG0000184200.01	37.02	3.64	14.88	44.46	11
FtPinG0000812400.01	48.35	4.40	16.48	30.77	3
FtPinG0002608200.01	29.34	6.16	17.03	47.46	5
FtPinG0009584000.01	54.05	2.60	12.72	30.64	8
FtPinG0001943900.01	14.86	5.41	20.27	59.46	0
FtPinG0008441300.01	50.00	2.86	15.24	31.90	10
FtPinG0000342600.01	50.68	4.39	17.23	27.70	6
FtPinG0001943500.01	32.11	5.26	15.79	46.84	2
FtPinG0005577300.01	46.06	3.94	15.16	34.84	10
FtPinG0001944100.01	53.91	4.35	13.04	28.70	10

苦荞蔗糖转运体家族FtSUCs的鉴定与生物信息学分析

Identification and bioinformatics analysis of sucrose transporter family FtSUCs in Tartary buckwheat

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