桃4CL基因家族鉴定及其在果实色泽发育和采后贮藏冷害中的表达分析

doi:10.3969/j.issn.1004-1524.20221604

浙江农业学报 ›› 2023, Vol. 35 ›› Issue (11): 2600-2610.DOI: 10.3969/j.issn.1004-1524.20221604

桃4CL基因家族鉴定及其在果实色泽发育和采后贮藏冷害中的表达分析

孔凡旺¹^,²(), 张志刚², 李伟², 陈玉峰², 王长江², 郑亚琴¹, 徐蒙¹^,^*()

1.临沂大学农林科学学院, 山东临沂 276000
2.邹城市自然资源和规划局, 山东济宁 273500

收稿日期:2022-11-11 出版日期:2023-11-25 发布日期:2023-12-04
作者简介:孔凡旺(1977—),男,山东邹城人,学士,高级工程师,主要从事林果病理和采后生理研究。E-mail: Zclcsdkfw@163.com
通讯作者: * 徐蒙,E-mail: xumeng@lyu.edu.cn
基金资助:
国家自然科学基金(32302621);山东省自然科学基金(ZR2021QC100);山东省现代农业产业技术体系(SDAIT-06-08);山东省高等学校青创科技计划创新团队(2021KJ055)

Identification of 4CL gene family in peach and its expression analysis in fruit coloration during development stage and chilling injury during post-harvest low temperature storage

KONG Fanwang¹^,²(), ZHANG Zhigang², LI Wei², CHEN Yufeng², WANG Changjiang², ZHENG Yaqin¹, XU Meng¹^,^*()

1. College of Agriculture and Forestry Sciences, Linyi University, Linyi 276000, Shandong, China
2. Zoucheng Natural Resources and Planning Bureau, Jining 273500, Shandong, China

Received:2022-11-11 Online:2023-11-25 Published:2023-12-04

摘要/Abstract

摘要：

4-香豆酸辅酶A连接酶(4-coumarate: CoA ligase, 4CL)在类黄酮和木质素等化合物代谢中具有重要作用。本研究基于桃基因组数据库系统鉴定4CL基因家族成员,从基因家族进化树的角度分析桃4CL基因家族的结构与潜在功能,结合转录组数据和qRT-PCR技术分析其在果实发育阶段类黄酮合成及采后冷害木质化调控中的功能。结果表明,基于桃基因组数据库鉴定出21个4CL基因家族成员,分布在8条染色体上;基因家族进化树分析表明,Pp4CL1与Pp4CL2属于第一亚家族,主要参与木质素合成,Pp4CL3-Pp4CL21属于第二亚家族,主要参与类黄酮的合成;启动子顺式元件分析发现其含有大量的非生物胁迫以及茉莉酸甲酯(MeJA)等激素响应元件。发育阶段的基因表达结果表明,Pp4CL8和 Pp4CL13的表达水平随发育阶段总体呈上升趋势,且在红肉品种中的表达量高于黄肉和白肉品种,猜测其可能参与花青素等类黄酮合成。采后低温贮藏阶段的基因表达结果表明,Pp4CL2可能在果实采后冷害木质化中发挥作用,程序性降温(low temperature conditioning,LTC)处理后Pp4CL8、Pp4CL10和Pp4CL14也可通过诱导类黄酮合成,减轻采后低温贮藏对果实造成的冷害。本研究将为桃果实色泽发育和采后保鲜提供理论依据。

关键词: 桃, 木质素, 黄酮, 基因表达

Abstract:

4CL (4-coumarate: CoA ligase) plays an important role in the metabolism of phenylalanine compounds such as flavonoids and lignin. In this study, the peach 4CL gene family members were systematically identified based on the peach genome database, and the structure and possible functions of the peach 4CLs were analyzed using the genetic family evolutionary analysis, combing transcriptome data and qRT-PCR techniques to analyze their functions in flavonoids synthesis at the fruit development stage and chilling-induced lignification regulation at postharvest. The results showed that 21 members of the 4CL gene family, namely Pp4CL1-Pp4CL21, were identified on 8 chromosomes based on the peach genome database. Phylogenetic analysis showed that Pp4CL1 and Pp4CL2 belong to the first subfamily, mainly involved in the synthesis of lignin, and Pp4CL3-Pp4CL21 belongs to the second subfamily, mainly involved in the synthesis of flavonoids. The cis-element analysis of the 4CL promoters found that it contains a large number of abiotic stress and MeJA-responsive and other hormone response elements. The gene expression of different peach varieties at the development stage showed that Pp4CL8 and Pp4CL13 increased generally, and higher expression was observed in blood-flesh varieties than in yellow-and milk-white-flesh varieties, suggesting that these genes may participate in the fruit flavonoids synthesis. The gene expression of peach fruit during post-harvest cold storage showed that Pp4CL2 may play a role in the chilling-induced lignification of fruit flesh after harvest. After low temperature conditioning (LTC) treatment, Pp4CL8, Pp4CL10 and Pp4CL14 may reduce the chilling injury of peach fruit during post-harvest cold storage by improving flavonoids content. This study provides a theoretical basis for the coloration of peach fruit and its postharvest preservation.

Key words: peach, lignin, flavonoids, gene expression

中图分类号:

S662.1

孔凡旺, 张志刚, 李伟, 陈玉峰, 王长江, 郑亚琴, 徐蒙. 桃4CL基因家族鉴定及其在果实色泽发育和采后贮藏冷害中的表达分析[J]. 浙江农业学报, 2023, 35(11): 2600-2610.

KONG Fanwang, ZHANG Zhigang, LI Wei, CHEN Yufeng, WANG Changjiang, ZHENG Yaqin, XU Meng. Identification of 4CL gene family in peach and its expression analysis in fruit coloration during development stage and chilling injury during post-harvest low temperature storage[J]. Acta Agriculturae Zhejiangensis, 2023, 35(11): 2600-2610.

图/表 11

表1 qRT-PCR基因表达引物序列

Table 1 Primer sequences for quantitative real-time PCR analysis

基因 Gene	正向引物 Forward primer (5'→3')	反向引物 Reverse primer (3'→5')
Pp4CL8 Pp4CL10 Pp4CL14 EF2	CCCACATACCCAATTGCCCA AAAGAGCGGCTACTGTCCAC TGGAATCTCTACATGCTCCTCAG AGCAGGCTCTTGGTGGTATCT	TGAGGGCAGGTTTGTTTGAGT GGAGGGAGAGAGCGAAATGG GGTGAGGTTAGCGTGAAGATGA GATTCAATGACGGGGAGGTAG

图1 桃4CL基因家族成员染色体分布情况桃4CL基因在染色体上的分布以红色字体突出显示,串联重复基因以灰色突出显示,片段复制以橘黄色直线连接。

Fig.1 Chromosome distribution of 4CL gene family members in peach The distribution of the peach 4CL gene on the chromosome is highlighted in red font, tandem repeat genes are highlighted in gray, and segment duplication is connected in orange straight lines.

表2 桃4CL基因家族成员蛋白理化性质

Table 2 Physical and chemical properties of 4CL gene family proteins in peach

基因名称 Gene name	登录号 GenBank	氨基酸数目 Amino acid	等电点 Isoelectric point	分子量 Molecular weight/u	不稳定系数 Instability index
Pp4CL1	XM_007222180.2	544	5.83	59 937.30	30.32
Pp4CL2	XM_007215457.2	551	5.84	59 981.39	37.07
Pp4CL3	XM_020570163.1	567	8.9	62 153.90	41.82
Pp4CL4	XM_020567696.1	572	6.32	62 409.91	38.71
Pp4CL5	XM_007219446.2	457	5.56	49 467.21	31.17
Pp4CL6	XM_007225695.2	548	6.06	59 512.92	36.62
Pp4CL7	XM_007203554.2	543	8.44	59 630.94	41.21
Pp4CL8	XM_007208395.2	542	8.63	59 168.68	37.81
Pp4CL9	XM_007222403.2	569	8.95	61 299.59	33.63
Pp4CL10	XM_007209025.2	557	7.26	61 211.61	53.40
Pp4CL11	XM_020569127.1	591	8.47	63 537.10	43.54
Pp4CL12	XM_007219420.2	569	5.73	61 733.01	42.35
Pp4CL13	XM_007209024.2	558	6.26	61 052.43	49.46
Pp4CL14	XM_020566011.1	635	6.56	69 746.76	36.41
Pp4CL15	XM_007221216.2	748	8.29	83 920.25	44.22
Pp4CL16	XM_020554905.1	748	8.29	83 920.25	44.22
Pp4CL17	XM_020554134.1	566	8.11	63 179.53	37.96
Pp4CL18	XM_020561814.1	555	7.23	60 667.33	38.26
Pp4CL19	XM_020564772.1	165	6.51	18 632.33	44.38
Pp4CL20	XM_007200387.2	218	6.6	25 006.86	38.89
Pp4CL21	XM_020570041.1	218	6.6	25 006.86	38.89

图2 桃4CL基因家族成员保守结构域分析与基因结构

Fig.2 Conservative domain analysis and gene structure of 4CL gene family members in peach

图3 桃4CL基因家族成员的保守基序

Fig.3 Conservative motifs of 4CL gene family members in peach

图4 桃4CL基因家族成员启动子的顺式作用元件

Fig.4 Cis-acting elements of peach 4CL gene family member promoters

图5 桃、杨树和拟南芥4CL基因家族成员共线性分析

Fig.5 Collinearity analysis of 4CL gene family members of peach, poplar and Arabidopsis

图6 桃4CL基因家族成员遗传进化分析

Fig.6 Phylogenetic analysis of 4CL gene family members in peach

图7 红肉、黄肉和白肉光核桃果肉发育阶段4CL基因表达模式 PH,硬核期;CE,细胞增大期;FR,果实成熟期。

Fig.7 4CL gene expression in the development stage of red, yellow and white flesh peaches PH,pit-harding; CE,cell enlargement; FR,fruit ripening.

图8 4CL基因家族成员在采后低温和LTC处理中的表达模式

Fig.8 Expression of 4CL gene family members in postharvest low temperature and LTC treatment

图9 qRT-PCR验证候选4CL基因在采后0 ℃(A)和LTC(B)处理中的相对表达水平

Fig.9 qRT-PCR verification of relative expression level of candidate 4CL genes at 0 ℃ (A) and LTC treatment (B)

参考文献 38

[1]	LAVHALE S G, KALUNKE R M, GIRI A P. Structural, functional and evolutionary diversity of 4-coumarate-CoA ligase in plants[J]. Planta, 2018, 248(5): 1063-1078.
[2]	SCHNEIDER K, HÖVEL K, WITZEL K, et al. The substrate specificity-determining amino acid code of 4-coumarate: CoA ligase[J]. Proceedings of the National Academy of Sciences of the United States of America, 2003, 100(14): 8601-8606.
[3]	LOZOYA E, HOFFMANN H, DOUGLAS C, et al. Primary structures and catalytic properties of isoenzymes encoded by the two 4-coumarate: CoA ligase genes in parsley[J]. European Journal of Biochemistry, 1988, 176(3): 661-667.
[4]	DOUGLAS C, HOFFMANN H, SCHULZ W, et al. Structure and elicitor or UV-light-stimulated expression of two 4-coumarate: CoA ligase genes in parsley[J]. The EMBO Journal, 1987, 6(5): 1189-1195.
[5]	LI Y, KIM J I, PYSH L, et al. Four isoforms of Arabidopsis 4-coumarate: CoA ligase have overlapping yet distinct roles in phenylpropanoid metabolism[J]. Plant Physiology, 2015, 169(4): 2409-2421
[6]	WANG Y Y, GUO L H, ZHAO Y J, et al. Systematic analysis and expression profiles of the 4-coumarate: CoA ligase (4CL) gene family in pomegranate (Punica granatum L.)[J]. International Journal of Molecular Sciences, 2022, 23(7): 3509.
[7]	XU B, ESCAMILLA-TREVIÑO L L, SATHITSUKSANOH N, et al. Silencing of 4-coumarate: coenzyme A ligase in switchgrass leads to reduced lignin content and improved fermentable sugar yields for biofuel production[J]. The New Phytologist, 2011, 192(3): 611-625.
[8]	FARCUH M, TAJIMA H, LERNO L A, et al. Changes in ethylene and sugar metabolism regulate flavonoid composition in climacteric and non-climacteric plums during postharvest storage[J]. Food Chemistry Molecular Sciences, 2022, 4: 100075.
[9]	WANG B, SUN W, LI Q S, et al. Genome-wide identification of phenolic acid biosynthetic genes in Salvia miltiorrhiza[J]. Planta, 2015, 241(3): 711-725.
[10]	VOELKER S L, LACHENBRUCH B, MEINZER F C, et al. Antisense down-regulation of 4CL expression alters lignification, tree growth, and saccharification potential of field-grown poplar[J]. Plant Physiology, 2010, 154(2): 874-886.
[11]	WANG H Z, XUE Y X, CHEN Y J, et al. Lignin modification improves the biofuel production potential in transgenic Populus tomentosa[J]. Industrial Crops and Products, 2012, 37(1): 170-177.
[12]	SHI R, SUN Y H, LI Q Z, et al. Towards a systems approach for lignin biosynthesis in Populus trichocarpa: transcript abundance and specificity of the monolignol biosynthetic genes[J]. Plant and Cell Physiology, 2010, 51(1): 144-163.
[13]	杨克彬, 单雪萌, 史晶晶, 等. 毛竹4-香豆酸辅酶A连接酶基因家族鉴定及表达分析[J]. 核农学报, 2021, 35(1): 72-82.
	YANG K B, SHAN X M, SHI J J, et al. Identification and expression analysis of 4CL gene family in Phyllostachys edulis[J]. Journal of Nuclear Agricultural Sciences, 2021, 35(1): 72-82. (in Chinese with English abstract)
[14]	曹运鹏, 方志, 李姝妹, 等. 砀山酥梨4CL基因家族的全基因组鉴定与分析[J]. 遗传, 2015, 37(7): 711-719.
	CAO Y P, FANG Z, LI S M, et al. Genome-wide identification and analyses of 4CL gene families in Pyrus bretschneideri Rehd[J]. Hereditas, 2015, 37(7): 711-719. (in Chinese with English abstract)
[15]	WANG K, YIN X R, ZHANG B, et al. Transcriptomic and metabolic analyses provide new insights into chilling injury in peach fruit[J]. Plant, Cell & Environment, 2017, 40(8): 1531-1551.
[16]	XU Q, YIN X R, ZENG J K, et al. Activator-and repressor-type MYB transcription factors are involved in chilling injury induced flesh lignification in loquat via their interactions with the phenylpropanoid pathway[J]. Journal of Experimental Botany, 2014, 65(15): 4349-4359.
[17]	LUO Z S, XU X L, YAN B F. Accumulation of lignin and involvement of enzymes in bamboo shoot during storage[J]. European Food Research and Technology, 2008, 226(4): 635-640.
[18]	周慧娟, 苏明申, 叶正文, 等. 桃果实采后生理生化及冷害研究进展[J]. 果树学报, 2017, 34(9): 1204-1212.
	ZHOU H J, SU M S, YE Z W, et al. Advances in the research into physiological and biochemical characteristics and chilling injury of peach fruits after harvest[J]. Journal of Fruit Science, 2017, 34(9): 1204-1212. (in Chinese with English abstract)
[19]	CHEN Z Z, CHEN Y C, CHOU Y H, et al. cDNA cloning and molecular characterization of 4-coumarate:coenzyme A ligase in Eucalyptus camaldulensis[J]. Taiwan Journal of Forest Science, 2006, 21(1): 87-100.
[20]	NEGISHI N, NANTO K, HAYASHI K, et al. Transcript abundances of LIM transcription factor, 4CL, CAld5H and CesAs affect wood properties in Eucalyptus globulus[J]. Silvae Genetica, 2011, 60: 288-296.
[21]	BRANDI F, BAR E, MOURGUES F, et al. Study of ‘Redhaven’ peach and its white-fleshed mutant suggests a key role of CCD4 carotenoid dioxygenase in carotenoid and norisoprenoid volatile metabolism[J]. BMC Plant Biology, 2011, 11: 24.
[22]	FALCHI R, VENDRAMIN E, ZANON L, et al. Three distinct mutational mechanisms acting on a single gene underpin the origin of yellow flesh in peach[J]. The Plant Journal, 2013, 76(2): 175-187.
[23]	JIAO Y, MA R, SHEN Z, et al. Gene regulation of anthocyanin biosynthesis in two blood-flesh peach (Prunus persica(L.) Batsch) cultivars during fruit development[J]. Journal of Zhejiang University. Science. B, 2014, 15(9): 809-819.
[24]	YAN J A, CAI Z X, SHEN Z J, et al. Proanthocyanidin monomers and cyanidin 3-O-glucoside accumulation in blood-flesh peach (Prunus persica (L.) Batsch) fruit[J]. Archives of Biological Sciences, 2017, 69(4): 611-617.
[25]	赵秀林, 臧程, 田义超, 等. 桃果实中花青素的研究进展[J]. 安徽农业科学, 2012, 40(10): 5735-5736, 5750.
	ZHAO X L, ZANG C, TIAN Y C, et al. Research progress of anthocyanin in peach fruit[J]. Journal of Anhui Agricultural Sciences, 2012, 40(10): 5735-5736, 5750. (in Chinese with English abstract)
[26]	YING H, SHI J, ZHANG S S, et al. Transcriptomic and metabolomic profiling provide novel insights into fruit development and flesh coloration in Prunus mira Koehne, a special wild peach species[J]. BMC Plant Biology, 2019, 19(1): 463.
[27]	CAO K, DING T Y, MAO D M, et al. Transcriptome analysis reveals novel genes involved in anthocyanin biosynthesis in the flesh of peach[J]. Plant Physiology and Biochemistry, 2018, 123: 94-102.
[28]	SHEN Z J, CONFOLENT C, LAMBERT P, et al. Characterization and genetic mapping of a new blood-flesh trait controlled by the single dominant locus DBF in peach[J]. Tree Genetics & Genomes, 2013, 9(6): 1435-1446.
[29]	王震光, 余义和, 郭大龙. 活性氧调控果实发育成熟的研究进展[J]. 浙江农业学报, 2020, 32(11): 2103-2110.
	WANG Z G, YU Y H, GUO D L. Advances in ROS promoting fruit development and ripening[J]. Acta Agriculturae Zhejiangensis, 2020, 32(11): 2103-2110. (in Chinese with English abstract)
[30]	YU J, CANG J, LU Q W, et al. ABA enhanced cold tolerance of wheat ‘dn1’ via increasing ROS scavenging system[J]. Plant Signaling & Behavior, 2020, 15(8): 1780403.
[31]	ALI KHAN R, KHAN M R, SAHREEN S, et al. Assessment of flavonoids contents and in vitro antioxidant activity of Launaea procumbens[J]. Chemistry Central Journal, 2012, 6(1): 43.
[32]	李煦, 白雪晴, 刘长霞, 等. 天然花青素的抗氧化机制及功能活性研究进展[J]. 食品安全质量检测学报, 2021, 12(20): 8163-8171.
	LI X, BAI X Q, LIU C X, et al. Research progress on antioxidant mechanism and functional activity of natural anthocyanin[J]. Journal of Food Safety & Quality, 2021, 12(20): 8163-8171. (in Chinese with English abstract)
[33]	SUN H Y, LI Y, FENG S Q, et al. Analysis of five rice 4-coumarate: coenzyme A ligase enzyme activity and stress response for potential roles in lignin and flavonoid biosynthesis in rice[J]. Biochemical and Biophysical Research Communications, 2013, 430(3): 1151-1156.
[34]	SUN S C, XIONG X P, ZHANG X L, et al. Correction to: characterization of the Gh4CL gene family reveals a role of Gh4CL7 in drought tolerance[J]. BMC Plant Biology, 2021, 21(1): 65.
[35]	ZHANG C H, MA T, LUO W C, et al. Identification of 4CL genes in desert poplars and their changes in expression in response to salt stress[J]. Genes, 2015, 6(3): 901-917.
[36]	JIN P, SUN C C, ZHENG Y H, et al. Effects of methyl jasmonate in combination with low temperature conditioning on chilling injury and active oxygen metabolism in loquat fruits[J]. Acta Horticulturae Sinica, 2012, 39(3): 461-468.
[37]	李永晖, 李捷, 冯丽丹, 等. 不同植物免疫诱抗剂对枸杞鲜果产量、抗病性和贮藏能力的差异比较[J]. 浙江农业学报, 2023, 35(1): 164-174.
	LI Y H, LI J, FENG L D, et al. Comparison of fruit yield, disease resistance and storage ability of Lycium bararum sprayed with different plant immune inducers[J]. Acta Agriculturae Zhejiangensis, 2023, 35(1): 164-174. (in Chinese with English abstract)
[38]	CHEN M S, GUO H M, CHEN S Q, et al. Methyl jasmonate promotes phospholipid remodeling and jasmonic acid signaling to alleviate chilling injury in peach fruit[J]. Journal of Agricultural and Food Chemistry, 2019, 67(35): 9958-9966.

桃4CL基因家族鉴定及其在果实色泽发育和采后贮藏冷害中的表达分析

Identification of 4CL gene family in peach and its expression analysis in fruit coloration during development stage and chilling injury during post-harvest low temperature storage

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