血耳及其宿主菌的分离与系统发育分析

doi:10.3969/j.issn.1004-1524.2021.03.09

浙江农业学报 ›› 2021, Vol. 33 ›› Issue (3): 447-453.DOI: 10.3969/j.issn.1004-1524.2021.03.09

血耳及其宿主菌的分离与系统发育分析

陈莎, 何贝贝, 陈启武, 李利^*()

长江大学生命科学学院,湖北荆州 434025

收稿日期:2020-06-09 出版日期:2021-04-02 发布日期:2021-03-25
通讯作者: 李利
作者简介:*, 李利,E-mail: lily2012@yangtzeu.edu.cn
陈莎(1979—),男,湖北荆州人,硕士,实验师,主要从事菌物资源和利用研究。E-mail: chensuo9803@126.com
基金资助:
湖北省科技计划(2019AHB067);国家自然科学基金(31730068)

Isolation and phylogenetic analysis of Tremella sanguinea and its fungal host

CHEN Suo, HE Beibei, CHEN Qiwu, LI Li^*()

College of Life Science, Yangtze University, Jingzhou 434025, China

Received:2020-06-09 Online:2021-04-02 Published:2021-03-25
Contact: LI Li

摘要/Abstract

摘要：

为研究血耳(Tremella sanguinea Y.B. Peng)及其宿主菌的分类地位,利用担孢子弹射法和耳木组织培养法从椴木栽培的血耳中分离得到1株酵母状菌株XE01和1株丝状真菌菌株XE02,分别对这两株菌株进行了菌落形态和显微形态观察及基于rDNA-ITS序列的系统发育分析。结果显示,XE01在PDA培养基上形成湿润、光滑的白色菌落;细胞为椭圆形,可见芽体;其ITS序列与褐银耳属(Phaeotremella)聚为一支,与P. neofoliacea 和P. eugeniae的遗传距离最小,亲缘关系最近,初步推测为血耳的酵母态型。菌株XE02在PDA培养基上形成白色绒毛状菌落;菌丝分枝少,有隔,可见单生的球状孢子和锁状联合;培养基中可见红褐色分泌物;其ITS序列与韧革菌属(Stereum)聚为一支,与皱韧革菌(S. rugosum)的遗传距离最小,亲缘关系最近,初步推测为血耳的宿主菌。研究为厘清血耳及其宿主菌的分类地位及种质资源鉴定提供了分子依据。

关键词: 血耳, 宿主, 形态, 分子系统发育

Abstract:

In order to study the taxonomic status of Tremella sanguinea Y.B. Peng and its fungal host, pure cultures, named as XE01 and XE02, were respectively obtained from basidiospores and basidiocarp tissues of Tremella sanguinea cultivated on Tilia sp. logs. The colony and microscopic morphology, as well as the molecular phylogeny based on rDNA-ITS of the two strains were then analyzed. It was found that the colonies of XE01 on PDA medium were white-creamy, smooth and moist; the cells were oval, budding and yeast-like. In the phylogenetic tree based on rDNA-ITS, XE01 was nested within in the clade of the genus Phaeotremella, and showed closer phylogenetic relationship to P. neofoliacea and P. eugeniae according to the genetic distances. The colonies of XE02 on PDA medium were fluffy and white; the hyphae were septate, and rarely branched; spherical spores and clamp connections were present; the inoculated agar medium was reddish brown due to the production of diffusible pigments. In the phylogenetic tree based on rDNA-ITS, XE02 was nested within the clade of the genus Stereum and showed closer phylogenetic relationship to S. rugosum according to the genetic distances. Based on these results, XE01 might be the free-living unicellular yeast state of T. sanguinea, and XE02 might be its fungal host. The results would contribute towards the classification of T. sanguinea and its fungal host, as well as the identification of their germplasm resources.

Key words: Tremella sanguinea, fungal host, morphology, molecular phylogeny

中图分类号:

S646

陈莎, 何贝贝, 陈启武, 李利. 血耳及其宿主菌的分离与系统发育分析[J]. 浙江农业学报, 2021, 33(3): 447-453.

CHEN Suo, HE Beibei, CHEN Qiwu, LI Li. Isolation and phylogenetic analysis of Tremella sanguinea and its fungal host[J]. Acta Agriculturae Zhejiangensis, 2021, 33(3): 447-453.

图/表 6

参考文献 26

[1]	陈士瑜 . 血耳及其人工栽培[J]. 中国食用菌, 1992,11(3):10-11.
	CHEN S Y . Tremella Sanguinea Y.B. Peng and its artificial cultivation[J]. Edible Fungi of China, 1992,11(3):10-11.(in Chinese)
[2]	彭寅斌 . 银耳属一新种[J]. 湖南师范大学自然科学学报, 1990,13(3):253-256.
	PENG Y B . A new species of the genus Tremella from China[J]. Journal of Natural Science of Hunan Normal University, 1990,13(3):253-256.(in Chinese with English abstract)
[3]	刘波 . 中国真菌志: 第二卷银耳目和花耳目[M]. 北京: 科学技术出版社, 1992: 90-91.
[4]	田云霞, 童江云, 汪威 , 等. 银耳属伴生现象研究进展[J]. 食用菌, 2019,41(4):1-3.
	TIAN Y X, TONG J Y, WANG W , et al. Research progress on commensalism of Tremella[J]. Edible Fungi, 2019,41(4):1-3.(in Chinese)
[5]	王玉万, 王云 . 银耳耳友菌降解木质纤维素的研究[J]. 生态学杂志, 1988,7(4):14-16.
	WANG Y W, WANG Y . Study on degradation of lignocollulose by cohabitant fungus of Tremella fuciformis[J]. Chinese Journal of Ecology, 1988,7(4):14-16.(in Chinese with English abstract)
[6]	王玉万, 王云 . 粗毛硬革菌木腐生理的研究[J]. 食用菌, 1989,5:19-20.
	WANG Y W, WANG Y . Research on the wood-rot physiology of Streum hirsutum[J]. Edible Fungi, 1989,5:19-20.(in Chinese)
[7]	宋燕娇, 林隆基, 杨立志 , 等. 一株阴环炭团菌(香灰菌)转化子形态变异的成因[J]. 菌物学报, 2019,38(12):2183-2194.
	SONG Y J, LIN L J, YANG L Z , et al. The cause of morphological mutation in a gene-deletion transformant of Annulohypoxylon stygium[J]. Mycosytema, 2019,38(12):2183-2194.(in Chinese with English abstract)
[8]	LIU X Z, WANG Q M, THEELEN B , et al. Phylogeny of tremellomycetous yeasts and related dimorphic and filamentous basidiomycetes reconstructed from multiple gene sequence analyses[J]. Studies in Mycology, 2015,81:1-26. DOI URL PMID
[9]	LIU X Z, WANG Q M, GÖKER M, et al. Towards an integrated phylogenetic classification of the Tremellomycetes[J]. Studies in Mycology, 2015,81:85-147. DOI URL PMID
[10]	谢世秀 . 血耳的分离培养[J]. 食用菌, 1983(2):8.
	XIE S X . Isolation and cultivation of Tremella sanguinea Y.B. Peng[J]. Edible Fungi, 1983(2):8.(in Chinese)
[11]	LIU W S, ZHOU Y, LIAO H , et al. A rapid DNA isolation procedure for small quantities of fresh leaf tissue[J]. American Journal of Botany, 2011,98(12):202-222.
[12]	WHITE T J, BRUNS T, LEE S , et al. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics[M] //PCR Protocols. Amsterdam: Elsevier, 1990: 315-322.
[13]	GARDES M, BRUNS T D . ITS primers with enhanced specificity for basidiomycetes-application to the identification of mycorrhizae and rusts[J]. Molecular Ecology, 1993,2(2):113-118. DOI URL PMID
[14]	LARKIN M A, BLACKSHIELDS G, BROWN N P , et al. Clustal W and Clustal X version 2.0[J]. Bioinformatics, 2007,23(21):2947-2948. DOI URL PMID
[15]	KUMAR S, STECHER G, LI M , et al. MEGA X: molecular evolutionary genetics analysis across computing platforms[J]. Molecular Biology and Evolution, 2018,35(6):1547-1549. DOI URL PMID
[16]	SPIRIN V, MALYSHEVA V, YURKOV A , et al. Studies in the Phaeotremella foliacea group (Tremellomycetes, Basidiomycota)[J]. Mycological Progress, 2018,17(4):451-466.
[17]	VU D, GROENEWALD M, SZÖKE S, et al. DNA barcoding analysis of more than 9 000 yeast isolates contributes to quantitative thresholds for yeast species and genera delimitation[J]. Studies in Mycology, 2016,85:91-105. DOI URL PMID
[18]	SCHOCH C L, ROBBERTSE B, ROBERT V , et al. Finding needles in haystacks: linking scientific names, reference specimens and molecular data for fungi[J]. Database-The Journal of Biological Databases and Curation, 2014: 1-21. DOI URL PMID
[19]	FINDLEY K, RODRIGUEZ-CARRES M, METIN B , et al. Phylogeny and phenotypic characterization of pathogenic Cryptococcus species and closely related saprobic taxa in the tremellales[J]. Eukaryotic Cell, 2009,8(3):353-361. DOI URL PMID
[20]	VU D, GROENEWALD M, DE VRIES M , et al. Large-scale generation and analysis of filamentous fungal DNA barcodes boosts coverage for kingdom fungi and reveak thresholds for fungal species and higher taxon delimitation[J]. Studies in Mycology, 2019,92:135-154. DOI URL PMID
[21]	温文婷, 贾定洪, 郭勇 , 等. 中国主栽银耳配对香灰菌的系统发育和遗传多样性[J]. 中国农业科学, 2010,43(3):552-558.
	WEN W T, JIA D H, GUO Y , et al. Phylogeny and genetic diversity of Annulohypoxylon spp. paired with cultivated Tremella fuciformis berk[J]. Scientia Agricultura Sinica, 2010,43(3):552-558.(in Chinese with English abstract)
[22]	谢宝贵, 应正河, 黄年来 , 等. 银耳伴生菌分类地位研究[J]. 菌物学报, 2005,24(增刊):73-77.
	XIE B G, YING Z H, HUANG N L , et al. Studies on systematic diversity of Tremella fuciformis cohabitant fungus[J]. Mycosystema, 2005,24(Suppl):73-77.(in Chinese with English abstract)
[23]	ABE A, ODA Y, ASANO K , et al. The molecular phylogeny of the genus Rhizopus based on rDNA sequences[J]. Bioscience, Biotechnology, and Biochemistry, 2006,70(10):2387-2393.
[24]	FELL J W, BOEKHOUT T, FONSECA A , et al. Biodiversity and systematics of basidiomycetous yeasts as determined by large-subunit rDNA D1/D2 domain sequence analysis[J]. International Journal of Systematic and Evolutionary Microbiology, 2000,50(3):1351-1371.
[25]	SCORZETTI G, FELL J W, FONSECA A , et al. Systematics of basidiomycetous yeasts: a comparison of large subunit D1/D2 and internal transcribed spacer rDNA regions[J]. FEMS Yeast Research, 2002,2(4):495-517. DOI URL PMID
[26]	NOBLES M K . Identification of cultures of wood-inhabiting hymenomycetes[J]. Canadian Journal of Botany, 1965,43(9):1097-1139.

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菌株 Strain	GenBank登录号 GenBank accession number
XE01	MN894289 (本研究This study)
XE02	MN894290 (本研究This study)
Phaeotremella foliacea DSM5493	MF580586^[16]
P. eugeniae LE303429	NR_158846^[16]
P. fimbriata Niemela 7897	MF076910^[16]
P. neofoliacea CBS8481	KY104488^[17]
P. skinneri CBS5029	NR_073211^[18]
P. frondosa LE206897	MF076907^[16]
P. mycophaga DSM104603	MF580589^[16]
P. pseudofoliacea CBS8227	KY104493^[17]
P. fagi CBS9964	NR_119558^[18]
P. mycetophiloides DSM5728	MF580587^[16]
Tremella globispora CBS6972	NR_155889^[19]
T. flava CBS8471	NR_155935^[17]
T. cinnabarina CBS8234	NR_155933^[17]
T.brasiliensis CBS8231	KY105675^[17]
T. fuciformis CBS6970	NR_155936^[17]
Stereum gausapatum CBS349.39	MH856041^[20]
S. sanguinolentum CBS529.50	MH856746^[20]
S. hirsutum FBCC1149	EU673087^[20]
S. armeniacum CBS944.96	MH862626^[20]
S. rugosum CBS108843	MH862811^[20]
Annulohypoxylon stygium XH1	FJ848850^[21]
香灰菌Hypoxylon sp.	N^[22]
Holtermannia corniformis CBS6979	AF410472^[8]
Rhizopus oryzae CBS112.07	AB097334^[23]

菌株 Strain	GenBank登录号 GenBank accession number
XE01	MN894289 (本研究This study)
XE02	MN894290 (本研究This study)
Phaeotremella foliacea DSM5493	MF580586^[16]
P. eugeniae LE303429	NR_158846^[16]
P. fimbriata Niemela 7897	MF076910^[16]
P. neofoliacea CBS8481	KY104488^[17]
P. skinneri CBS5029	NR_073211^[18]
P. frondosa LE206897	MF076907^[16]
P. mycophaga DSM104603	MF580589^[16]
P. pseudofoliacea CBS8227	KY104493^[17]
P. fagi CBS9964	NR_119558^[18]
P. mycetophiloides DSM5728	MF580587^[16]
Tremella globispora CBS6972	NR_155889^[19]
T. flava CBS8471	NR_155935^[17]
T. cinnabarina CBS8234	NR_155933^[17]
T.brasiliensis CBS8231	KY105675^[17]
T. fuciformis CBS6970	NR_155936^[17]
Stereum gausapatum CBS349.39	MH856041^[20]
S. sanguinolentum CBS529.50	MH856746^[20]
S. hirsutum FBCC1149	EU673087^[20]
S. armeniacum CBS944.96	MH862626^[20]
S. rugosum CBS108843	MH862811^[20]
Annulohypoxylon stygium XH1	FJ848850^[21]
香灰菌Hypoxylon sp.	N^[22]
Holtermannia corniformis CBS6979	AF410472^[8]
Rhizopus oryzae CBS112.07	AB097334^[23]

血耳及其宿主菌的分离与系统发育分析

Isolation and phylogenetic analysis of Tremella sanguinea and its fungal host

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