血耳及其宿主菌的分离与系统发育分析

doi:10.3969/j.issn.1004-1524.2021.03.09

浙江农业学报 ›› 2021, Vol. 33 ›› Issue (3): 447-453.DOI: 10.3969/j.issn.1004-1524.2021.03.09

血耳及其宿主菌的分离与系统发育分析

陈莎, 何贝贝, 陈启武, 李利^*()

长江大学生命科学学院,湖北荆州 434025

收稿日期:2020-06-09 出版日期:2021-04-02 发布日期:2021-03-25
通讯作者: 李利
作者简介:*, 李利,E-mail: lily2012@yangtzeu.edu.cn
陈莎(1979—),男,湖北荆州人,硕士,实验师,主要从事菌物资源和利用研究。E-mail: chensuo9803@126.com
基金资助:
湖北省科技计划(2019AHB067);国家自然科学基金(31730068)

Isolation and phylogenetic analysis of Tremella sanguinea and its fungal host

CHEN Suo, HE Beibei, CHEN Qiwu, LI Li^*()

College of Life Science, Yangtze University, Jingzhou 434025, China

Received:2020-06-09 Online:2021-04-02 Published:2021-03-25
Contact: LI Li

摘要/Abstract

摘要：

为研究血耳(Tremella sanguinea Y.B. Peng)及其宿主菌的分类地位,利用担孢子弹射法和耳木组织培养法从椴木栽培的血耳中分离得到1株酵母状菌株XE01和1株丝状真菌菌株XE02,分别对这两株菌株进行了菌落形态和显微形态观察及基于rDNA-ITS序列的系统发育分析。结果显示,XE01在PDA培养基上形成湿润、光滑的白色菌落;细胞为椭圆形,可见芽体;其ITS序列与褐银耳属(Phaeotremella)聚为一支,与P. neofoliacea 和P. eugeniae的遗传距离最小,亲缘关系最近,初步推测为血耳的酵母态型。菌株XE02在PDA培养基上形成白色绒毛状菌落;菌丝分枝少,有隔,可见单生的球状孢子和锁状联合;培养基中可见红褐色分泌物;其ITS序列与韧革菌属(Stereum)聚为一支,与皱韧革菌(S. rugosum)的遗传距离最小,亲缘关系最近,初步推测为血耳的宿主菌。研究为厘清血耳及其宿主菌的分类地位及种质资源鉴定提供了分子依据。

关键词: 血耳, 宿主, 形态, 分子系统发育

Abstract:

In order to study the taxonomic status of Tremella sanguinea Y.B. Peng and its fungal host, pure cultures, named as XE01 and XE02, were respectively obtained from basidiospores and basidiocarp tissues of Tremella sanguinea cultivated on Tilia sp. logs. The colony and microscopic morphology, as well as the molecular phylogeny based on rDNA-ITS of the two strains were then analyzed. It was found that the colonies of XE01 on PDA medium were white-creamy, smooth and moist; the cells were oval, budding and yeast-like. In the phylogenetic tree based on rDNA-ITS, XE01 was nested within in the clade of the genus Phaeotremella, and showed closer phylogenetic relationship to P. neofoliacea and P. eugeniae according to the genetic distances. The colonies of XE02 on PDA medium were fluffy and white; the hyphae were septate, and rarely branched; spherical spores and clamp connections were present; the inoculated agar medium was reddish brown due to the production of diffusible pigments. In the phylogenetic tree based on rDNA-ITS, XE02 was nested within the clade of the genus Stereum and showed closer phylogenetic relationship to S. rugosum according to the genetic distances. Based on these results, XE01 might be the free-living unicellular yeast state of T. sanguinea, and XE02 might be its fungal host. The results would contribute towards the classification of T. sanguinea and its fungal host, as well as the identification of their germplasm resources.

Key words: Tremella sanguinea, fungal host, morphology, molecular phylogeny

中图分类号:

S646

陈莎, 何贝贝, 陈启武, 李利. 血耳及其宿主菌的分离与系统发育分析[J]. 浙江农业学报, 2021, 33(3): 447-453.

CHEN Suo, HE Beibei, CHEN Qiwu, LI Li. Isolation and phylogenetic analysis of Tremella sanguinea and its fungal host[J]. Acta Agriculturae Zhejiangensis, 2021, 33(3): 447-453.

图/表 6

参考文献 26

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菌株 Strain	GenBank登录号 GenBank accession number
XE01	MN894289 (本研究This study)
XE02	MN894290 (本研究This study)
Phaeotremella foliacea DSM5493	MF580586^[16]
P. eugeniae LE303429	NR_158846^[16]
P. fimbriata Niemela 7897	MF076910^[16]
P. neofoliacea CBS8481	KY104488^[17]
P. skinneri CBS5029	NR_073211^[18]
P. frondosa LE206897	MF076907^[16]
P. mycophaga DSM104603	MF580589^[16]
P. pseudofoliacea CBS8227	KY104493^[17]
P. fagi CBS9964	NR_119558^[18]
P. mycetophiloides DSM5728	MF580587^[16]
Tremella globispora CBS6972	NR_155889^[19]
T. flava CBS8471	NR_155935^[17]
T. cinnabarina CBS8234	NR_155933^[17]
T.brasiliensis CBS8231	KY105675^[17]
T. fuciformis CBS6970	NR_155936^[17]
Stereum gausapatum CBS349.39	MH856041^[20]
S. sanguinolentum CBS529.50	MH856746^[20]
S. hirsutum FBCC1149	EU673087^[20]
S. armeniacum CBS944.96	MH862626^[20]
S. rugosum CBS108843	MH862811^[20]
Annulohypoxylon stygium XH1	FJ848850^[21]
香灰菌Hypoxylon sp.	N^[22]
Holtermannia corniformis CBS6979	AF410472^[8]
Rhizopus oryzae CBS112.07	AB097334^[23]

菌株 Strain	GenBank登录号 GenBank accession number
XE01	MN894289 (本研究This study)
XE02	MN894290 (本研究This study)
Phaeotremella foliacea DSM5493	MF580586^[16]
P. eugeniae LE303429	NR_158846^[16]
P. fimbriata Niemela 7897	MF076910^[16]
P. neofoliacea CBS8481	KY104488^[17]
P. skinneri CBS5029	NR_073211^[18]
P. frondosa LE206897	MF076907^[16]
P. mycophaga DSM104603	MF580589^[16]
P. pseudofoliacea CBS8227	KY104493^[17]
P. fagi CBS9964	NR_119558^[18]
P. mycetophiloides DSM5728	MF580587^[16]
Tremella globispora CBS6972	NR_155889^[19]
T. flava CBS8471	NR_155935^[17]
T. cinnabarina CBS8234	NR_155933^[17]
T.brasiliensis CBS8231	KY105675^[17]
T. fuciformis CBS6970	NR_155936^[17]
Stereum gausapatum CBS349.39	MH856041^[20]
S. sanguinolentum CBS529.50	MH856746^[20]
S. hirsutum FBCC1149	EU673087^[20]
S. armeniacum CBS944.96	MH862626^[20]
S. rugosum CBS108843	MH862811^[20]
Annulohypoxylon stygium XH1	FJ848850^[21]
香灰菌Hypoxylon sp.	N^[22]
Holtermannia corniformis CBS6979	AF410472^[8]
Rhizopus oryzae CBS112.07	AB097334^[23]

血耳及其宿主菌的分离与系统发育分析

Isolation and phylogenetic analysis of Tremella sanguinea and its fungal host

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