口服三七总皂甙对大黄鱼免疫功能的影响

doi:10.3969/j.issn.1004-1524.2021.04.06

浙江农业学报 ›› 2021, Vol. 33 ›› Issue (4): 610-617.DOI: 10.3969/j.issn.1004-1524.2021.04.06

口服三七总皂甙对大黄鱼免疫功能的影响

陈秀霞(), 池洪树, 许斌福, 谢岸桦, 方冬兰, 龚晖^*()

福建省农业科学院生物技术研究所,福建福州 350003

收稿日期:2020-11-10 出版日期:2021-04-25 发布日期:2021-04-25
通讯作者: 龚晖
作者简介:*龚晖,E-mail:ghxfjm@163.com
陈秀霞(1977—),女,福建尤溪人,硕士,副研究员,从事水产病害防治研究。E-mail:xiuxiachen@163.com
基金资助:
福建省属公益类科研院所专项(2018R1019-6);福建省农业科学院项目(AC2017-1)

Effects of oral Panax notoginseng saponins on immunity of large yellow croaker (Pseudosciaena crocea)

CHEN Xiuxia(), CHI Hongshu, XU Binfu, XIE Anhua, FANG Donglan, GONG Hui^*()

Biotechnology Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China

Received:2020-11-10 Online:2021-04-25 Published:2021-04-25
Contact: GONG Hui

摘要/Abstract

摘要：

将含不同浓度三七总皂甙(PNS)的饲料(0、10、50、250 mg·kg^-1)连续14 d投喂大黄鱼,检测肝、脾、头肾组织部分免疫相关基因的表达,以及血清部分免疫因子的活性,结合人工注射感染变形假单胞菌的抵抗力,评估口服PNS对大黄鱼免疫功能的影响。实验结果显示,各浓度组大黄鱼血清超氧化物歧化酶(SOD)、溶菌酶(LZM)、酸性磷酸酶(ACP)和碱性磷酸酶(ALP)活性与对照组相比无显著差异;高浓度组(250 mg·kg^-1)大黄鱼脾组织粘病毒抗性蛋白(Mx)的相对表达水平显著高于对照组,中浓度组(50 mg·kg^-1)脾组织和低浓度组(10 mg·kg^-1)头肾组织的Mx相对表达水平略高于对照组,但差异不显著;各浓度组各组织肿瘤坏死因子α链(TNF-α)的相对表达水平与对照组无显著差异,但均低于对照组;各浓度组各组织中白细胞介素10(IL-10)和主要组织相容性复合体Ⅱ类α链(MHCⅡ-α)的相对表达水平与对照组无显著差异。感染变形假单胞菌后各浓度组大黄鱼的死亡率与对照组无显著差异。结果表明,饲料中添加10~250 mg·kg^-1的PNS连续投喂14 d未能明显增强大黄鱼免疫机能。

关键词: 三七总皂甙, 大黄鱼, 免疫

Abstract:

In this study, large yellow croaker (Pseudosciaena crocea) were fed with diets containing 0,10,50,250 mg·kg^-1 Panax notoginseng saponins (PNS) for 14 d. To evaluate the effects of PNS on the immunity of large yellow croaker, the expression of some immune-related genes in the liver, spleen and head kidney tissues, the activities of some serum immune factors and the resistance against Pseudomonas plecoglossicida were determined. Results showed that the activities of serum superoxide dismutase (SOD), lysozyme (LZM), acp acid phosphatase (ACP) and alkaline phosphatase (AKP) in the experimental groups were not significantly different from the control group; the expression of Mx in spleen of fish fed with 250 mg·kg ^-1 PNS was significantly higher than that of control; the expression of Mx in spleen of fish fed with 50 mg·kg ^-1 PNS and in head-kidney of fish fed with 10 mg·kg^-1 PNS were higher than that of control, but not significantly; the expression of TNF-α in all the exprimental groups were lower than that of control, but not significantly; the expression of IL-10 and MHCⅡ-α in all the exprimental groups were not significantly different from the control. The mortalities of the exprimental fish challenged with P. plecoglossicida were not significantly different from the control. It was indicated that feeding diets containing 10-250 mg·kg ^-1 PNS for 14 d did not improve the immunity of large yellow croaker

Key words: Panax notoginseng saponins, large yellow croaker (Pseudosciaena crocea), immunity

中图分类号:

S965.322

陈秀霞, 池洪树, 许斌福, 谢岸桦, 方冬兰, 龚晖. 口服三七总皂甙对大黄鱼免疫功能的影响[J]. 浙江农业学报, 2021, 33(4): 610-617.

CHEN Xiuxia, CHI Hongshu, XU Binfu, XIE Anhua, FANG Donglan, GONG Hui. Effects of oral Panax notoginseng saponins on immunity of large yellow croaker (Pseudosciaena crocea)[J]. Acta Agriculturae Zhejiangensis, 2021, 33(4): 610-617.

图/表 6

表1 荧光定量PCR引物序列

Table 1 Primers used for real-time PCR

基因 Genes	引物核酸序列 Nucleic acid sequence of primers(5'-3')
Mx	上游引物 Forward primer: AGTTTTCCTCTTATTCGCCTGATTTTT
	下游引物 Reverse primer: TAAAAAGAACAAGTCAGCCAGGTGC
MHCⅡ-α	上游引物 Forward primer: TCAGCTGCACTCGTTCC
	下游引物 Reverse primer: CCAGCATCAACGTTCCTC
TNF-α	上游引物 Forward primer: TAAGAACACCTACGAGACCATCCTGC
	下游引物 Reverse primer: CTCTTCTCCATCACCGTCTTCAGTTT
IL-10	上游引物 Forward primer: CATCAACAGCATTTTAGATTTTTACC
	下游引物 Reverse primer: CTGAGTGTAAGTGGAGTTTAGGTTTG
β-actin	上游引物 Forward primer: GACCTGACAGACTACCTCATG
	下游引物 Reverse primer: AGTTGAAGGTGGTCTCGTGGA

图1 大黄鱼血清部分非特异免疫因子活性各柱状图上方无相同小写字母的表示各处理组间差异显著(P<0.05)。处理组0、1、2、3分别表示饲料中添加了0、10、50、250 mg·kg-1 PNS。下同。

Fig.1 Activities of serum nonspecific immune parameters of large yellow croaker Different lowercase letters above the columns represent statistically significant (P<0.05) differences among treatments. The treatment group 0, 1, 2, 3 represented the treatment with diets containing 0, 10, 50, 250 mg·kg-1 PNS. The same as below.

图2 大黄鱼肝组织部分免疫因子相对表达水平

Fig.2 Relative expression levels of immune related factors in liver

图3 大黄鱼脾组织部分免疫因子相对表达水平

Fig.3 Relative expression levels of immune related factors in spleen

图4 头肾组织部分免疫因子相对表达水平

Fig.4 Relative expression levels of immune related factors in head-kidney

图5 大黄鱼感染变形假单胞菌后每日死亡率

Fig.5 Mortality per day of large yellow croaker challenged with Pseudomonas plecoglossicida

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口服三七总皂甙对大黄鱼免疫功能的影响

Effects of oral Panax notoginseng saponins on immunity of large yellow croaker (Pseudosciaena crocea)

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