不同植物生长调节剂对柿砧亚林6号硬枝扦插生根的影响

doi:10.3969/j.issn.1004-1524.2021.07.11

浙江农业学报 ›› 2021, Vol. 33 ›› Issue (7): 1256-1263.DOI: 10.3969/j.issn.1004-1524.2021.07.11

不同植物生长调节剂对柿砧亚林6号硬枝扦插生根的影响

吴开云(), 龚榜初^*(), 徐阳, 章平生

中国林业科学研究院亚热带林业研究所,浙江杭州 311400

收稿日期:2020-07-16 出版日期:2021-07-25 发布日期:2021-08-06
通讯作者: 龚榜初
作者简介:*龚榜初,E-mail: gongbc@126.com
吴开云(1963—),男,江苏江都人,副研究员,从事经济林育种与栽培研究。E-mail: wukaiyun99@163.com
基金资助:
国家重点研发计划(2019YFD1001204);国家林业与草原局林业科学技术推广项目(〔2019〕46)

Effects of different plant growth regulators on hardwood cuttage of persimmon rootstock Yalin 6

WU Kaiyun(), GONG Bangchu^*(), XU Yang, ZHANG Pingsheng

Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou 311400, China

Received:2020-07-16 Online:2021-07-25 Published:2021-08-06
Contact: GONG Bangchu

摘要/Abstract

摘要：

从幼化的砧木采穗圃采集亚林6号插穗,进行硬枝扦插生根试验。利用植物生长调节IBA、ABT1、NAA、GGR6、IAA剂配制的不同浓度溶液及作为对照的清水浸泡插条切口,而后扦插于无菌基质。遮阴拱膜保湿环境下抚育45 d及露天滴灌环境下抚育15 d后,对生根效果进行取样检测、差异显著性分析和多重比较分析。结果表明,不同处理之间扦插生根效果差异显著,IAA、IBA、GGR6等在合适配比下有较高的生根率和萌梢生长量。其中处理A1、G3、G2生根率分别达到75.6%、56.9%和52.8%,均显著高于其他处理;生根率和愈伤发生率的规律不一致;生根类型有皮部生根、愈伤生根、混合生根3种类型;实验中扦插生根以皮部生根为主,混合生根为辅;IAA等处理有利于提高皮部生根发生,NAA、添加腐植酸的IBA等处理有利于促进愈伤组织生根。IAA 250 mg·L^-1溶液处理的扦插生根效果最好,GGR6 500 mg·L^-1扦插生根效果次之。综合成本优势和操作方便性,建议生产中使用GGR6 500 mg·L^-1处理培育柿砧木扦插苗。

关键词: 甜柿, 砧木, 扦插, 生根率, 愈伤组织

Abstract:

The cuttings of Yalin No.6 were collected from the nursery of immature rootstock, and the rooting experiment of hardwood cutting was carried out. IBA, ABT1, NAA, GGR6, IAA and other plant growth regulators were used to prepare different concentrations of solution and water was used as the control for the cutting immersion, then the cuttings were soaked and cut in sterile medium. After 45 days of shading film moisturizing environment and 15 days of open-air drip irrigation environment, the rooting effect was detected by sampling, significant difference analysis and multiple comparative analysis. The results showed that there were significant differences in rooting effect among different treatments. IAA, IBA and GGR6 had higher rooting rate and sprout growth under appropriate ratio. The rooting rates of A1, G3 and G2 were 75.6%, 56.9% and 52.8% respectively, which were significantly higher than those of other treatments. The changes of rooting rate and callus occurrence rate were not consistent. There are three types of rooting: cortex rooting, callus rooting and mixed rooting. In the experiment, the cutting rooting was mainly cortex rooting, supplemented by mixed rooting. IAA and other treatments can improve the cortex rooting, while NAA and IBA with humic acid could promote the rooting of callus. IAA and other treatments can improve the rooting rate of cortex, 250 mg · L^-1 IAA treatment had the best rooting effect, 500 mg · L^-1 GGR6 treatment was the second. Considering the cost advantage and operation convenience, it was suggested to use 500 mg · L^-1 GGR6 treatment to cultivate persimmon rootstock cuttings.

Key words: persimmon, rootstock, cuttage, rooting rate, callus

中图分类号:

S665.2

吴开云, 龚榜初, 徐阳, 章平生. 不同植物生长调节剂对柿砧亚林6号硬枝扦插生根的影响[J]. 浙江农业学报, 2021, 33(7): 1256-1263.

WU Kaiyun, GONG Bangchu, XU Yang, ZHANG Pingsheng. Effects of different plant growth regulators on hardwood cuttage of persimmon rootstock Yalin 6[J]. Acta Agriculturae Zhejiangensis, 2021, 33(7): 1256-1263.

图/表 8

参考文献 20

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处理 Treatment	试剂 Agentia	浓度 Concentration/(mg·L^-1)	处理 Treatment	试剂 Agentia	浓度 Concentration/(mg·L^-1)
A1	IAA	250	H1	IBA/腐植酸Humic acid	150/100
A2	IAA	500	H2	IBA/腐植酸Humic acid	300/200
A3	IAA	1 000	N1	NAA	125
B1	IBA	150	N2	NAA	250
B2	IBA	300	N3	NAA	500
B3	IBA	600	T1	ABT1	250
G1	GGR6	250	T2	ABT1	500
G2	GGR6	500	T3	ABT1	1 000
G3	GGR6	1 000	CK	纯净水Pure water	0

处理 Treatment	试剂 Agentia	浓度 Concentration/(mg·L^-1)	处理 Treatment	试剂 Agentia	浓度 Concentration/(mg·L^-1)
A1	IAA	250	H1	IBA/腐植酸Humic acid	150/100
A2	IAA	500	H2	IBA/腐植酸Humic acid	300/200
A3	IAA	1 000	N1	NAA	125
B1	IBA	150	N2	NAA	250
B2	IBA	300	N3	NAA	500
B3	IBA	600	T1	ABT1	250
G1	GGR6	250	T2	ABT1	500
G2	GGR6	500	T3	ABT1	1 000
G3	GGR6	1 000	CK	纯净水Pure water	0

指标 Index	根系干重 Weight of dried root	根系发生数量 Number of root	新梢粗度 Diameter of shoot	新梢高度 Length of shoot	根系长度 Length of rooting	成活率 Survival rate	生根率 Rooting rate	愈伤发生率 Callus formation rate
根系干重根系干重	1
Weight of dried root
根系发生数量Number of root	0.815^**	1
新梢粗度Diameter of shoot	0.664^**	0.764^**	1
新梢高度Length of shoot	0.637^**	0.748^**	0.957^**	1
根系长度Length of rooting	0.728^**	0.837^**	0.875^**	0.926^**	1
成活率Survival rate	0.686^**	0.782^**	0.974^**	0.942^**	0.861^**	1
生根率Rooting rate	0.751^**	0.893^**	0.938^**	0.931^**	0.928^**	0.962^**	1
愈伤发生率Callus formation rate	0.343^**	0.491^**	0.406^**	0.395^**	0.427^**	0.342^**	0.365^**	1

指标 Index	根系干重 Weight of dried root	根系发生数量 Number of root	新梢粗度 Diameter of shoot	新梢高度 Length of shoot	根系长度 Length of rooting	成活率 Survival rate	生根率 Rooting rate	愈伤发生率 Callus formation rate
根系干重根系干重	1
Weight of dried root
根系发生数量Number of root	0.815^**	1
新梢粗度Diameter of shoot	0.664^**	0.764^**	1
新梢高度Length of shoot	0.637^**	0.748^**	0.957^**	1
根系长度Length of rooting	0.728^**	0.837^**	0.875^**	0.926^**	1
成活率Survival rate	0.686^**	0.782^**	0.974^**	0.942^**	0.861^**	1
生根率Rooting rate	0.751^**	0.893^**	0.938^**	0.931^**	0.928^**	0.962^**	1
愈伤发生率Callus formation rate	0.343^**	0.491^**	0.406^**	0.395^**	0.427^**	0.342^**	0.365^**	1

变异来源 Source of variation	平方和 Sum of squares	自由度 Degree of freedom	均方 Mean square	F值 F value	P值 P value
不同处理Different treatments	26 850.096	17	1 579.417^**	27.982	0
生长调节剂Kinds of plant growth regulator	3 208.319	6	534.720^**	9.473	0
生长调节剂浓度梯度Concentration of plant growth regulator	5 321.076	6	886.846^**	15.712	0
误差Error	6 095.980	234	56.444

不同植物生长调节剂对柿砧亚林6号硬枝扦插生根的影响

Effects of different plant growth regulators on hardwood cuttage of persimmon rootstock Yalin 6

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