热应激与喷淋-风扇系统对不同泌乳阶段奶牛生理和生产性能的影响

doi:10.3969/j.issn.1004-1524.2021.09.04

浙江农业学报 ›› 2021, Vol. 33 ›› Issue (9): 1602-1610.DOI: 10.3969/j.issn.1004-1524.2021.09.04

热应激与喷淋-风扇系统对不同泌乳阶段奶牛生理和生产性能的影响

沈留红¹(), 程李杰¹, 尤留超¹, 雍康², 罗正中¹, 陈久兵¹, 骆巧¹, 余树民¹, 曹随忠¹^,^*()

1.四川农业大学动物医学院动物疫病与人类健康四川省重点实验室/奶牛疾病研究中心,四川成都 611130
2.重庆三峡职业学院动物科技学院,重庆 404155

收稿日期:2020-11-10 出版日期:2021-09-25 发布日期:2021-10-09
通讯作者: 曹随忠
作者简介:* 曹随忠,E-mail: suizhongcao@sicau.edu.cn
沈留红(1979—),男,江苏如皋人,博士,副教授,研究方向为反刍动物疾病与繁殖新技术。E-mail: shenlh@sicau.edu.cn
基金资助:
四川省科技厅科技计划(2019YJ0650);国家重点研发计划(2018YFD0500900);四川农业大学本科科研兴趣培养计划(KY2021140)

Effects of heat stress and spray-fan cooling system on physiology and production efficiency of dairy cows in different lactation stages

SHEN Liuhong¹(), CHENG Lijie¹, YOU Liuchao¹, YONG Kang², LUO Zhengzhong¹, CHEN Jiubing¹, LUO Qiao¹, YU Shumin¹, CAO Suizhong¹^,^*()

1. The Key Laboratory of Animal Disease and Human Health of Sichuan Province/The Medical Research Center for Cow Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
2. College of Animal Science and Technology, Chongqing Three Gorges Vocational College, Chongqing 404155, China

Received:2020-11-10 Online:2021-09-25 Published:2021-10-09
Contact: CAO Suizhong

摘要/Abstract

摘要：

为比较热应激与喷淋-风扇系统对热应激期不同泌乳期奶牛生理、生产性能、发病率的影响,将泌乳牛按泌乳时间分为新产牛(0~21 d)、泌乳前期(22~120 d)、泌乳中期(121~200 d)、泌乳后期(201~305 d)奶牛,分别随机选择体况、年龄、泌乳时间相近的健康经产(2~4胎)各泌乳阶段奶牛10头,共计40头作为试验对象。按养殖场日常饲养和管理方式,不做人工干预,热应激期采用喷淋-风扇系统降温。每日测定牛舍温度、相对湿度、风速、直肠温度、呼吸频率、日产奶量、日大缸奶乳成分、日新发肢蹄病、酮病、临床型乳房炎和隐性型乳房炎等。结果表明:喷淋-风扇系统下,热应激期牛舍内温度接近30 ℃,相对湿度为82%,风速达1.35 m·s^-1,湿热指数处于中度热应激。热应激期,各泌乳期奶牛直肠温度平均分别升高0.9、0.7、0.6、0.5 ℃,呼吸频率平均分别上升32.9、29.1、29.3、28.5次·min^-1,较非热应激期均极显著(P<0.01)升高;新产牛、泌乳前期和中期奶牛日单产均极显著(P<0.01)下降,降幅分别为9.17%、18.97%和13.23%,而泌乳后期奶牛无显著变化(P>0.05),新产牛泌乳启动时奶产量下降,各阶段泌乳牛泌乳峰值均降低;全群泌乳牛乳脂率、脂蛋比均极显著(P<0.01)下降,乳蛋白显著(P<0.05)降低,乳脂率、脂蛋比、乳蛋白含量分别降低0.29百分点、5.23%和1.61%;全群泌乳牛肢蹄病、酮病、临床型乳房炎和隐性型乳房炎发病率分别升高5.77、0.78、2.60和5.77百分点。结果说明:喷淋-风扇系统对舍内热环境改善不显著;热应激影响新产牛和泌乳前期、中期奶牛直肠温度和呼吸频率,使新产牛泌乳启动时产奶量下降,各阶段泌乳牛产奶峰值降低;全群泌乳牛乳品质降低,发病率升高。

关键词: 喷淋-风扇系统, 奶牛, 热应激, 生理参数, 产奶性能

Abstract:

To investigate the influence of heat stress period and spray-fan cooling system on physiology, milk production and morbidity of dairy cows (in different lactation stages), lactating cows were divided as late perinatal (0-21 d), the early lactation (22-120 d), the mid lactation (121-200 d), and the late lactation (201-305 d) according to days in milk. Ten healthy cows (with similar body condition, ages, lactation and 2-4 parities) were selected in each lactation stage (the total was 40) in this study. All cows were managed as usual without manual intervention, spray-fan cooling system was used during the heat stress period. Temperature, relative humidity, wind speed of the cowshed, rectal temperature, respiratory rates, milk production, milk composition, new cases of hoof disease, ketosis, clinical mastitis and subclinical mastitis were recorded or measured every day during experiment. The results were as follows: Average temperature in cowshed during the heat stress period was nearly 30 ℃ under spray-fan cooling system, relative humidity was 82%, wind speed was 1.35 m·s^-1 and temperature humidity index (THI) was within the range of moderate heat stress; The average rectal temperature of cows in each lactation stage increased by 0.9 ℃, 0.7 ℃, 0.6 ℃ and 0.5 ℃, respectively, and respiratory rate increased by an average of 32.9, 29.1, 29.3 and 28.5 times per minute, respectively, which was significantly higher than those in non-heat stress period (P<0.01); Average daily milk yields in late-perinatal, early-lactation and mid-lactation decreased significantly (P<0.01) during heat stress period (with a decrease of 9.17%, 18.97% and 13.23%, respectively), whereas there was no significant change in late lactation stage (P>0.05); Initial milk yield of late-perinatal cows decreased, peak value of milk production decreased in all stages. Milk-fat percentage and milk fat/protein of all cows decreased significantly (P<0.01), milk protein also decreased significantly (P<0.05), the decreases were 0.29 percent, 5.23% and 1.61%, respectively; Incidence of hoof disease, ketosis, clinical mastitis and subclinical mastitis of all cows increased by 5.77, 0.78, 2.60 and 5.77 percent, respectively. In conclusion, spray-fan cooling system could not significantly improve the thermal environment of cowshed. Heat stress greatly impacted the rectal temperature and respiratory rate of late-perinatal cows, early-lactation cows and mid-lactation cow, leading to the decrease of initial milk production, peak milk yield of cows in all lactation stages and milk quality, and the increase of morbidity.

Key words: spray-fan cooling system, dairy cows, heat stress, physiological indexes, milk production

中图分类号:

S854.5

沈留红, 程李杰, 尤留超, 雍康, 罗正中, 陈久兵, 骆巧, 余树民, 曹随忠. 热应激与喷淋-风扇系统对不同泌乳阶段奶牛生理和生产性能的影响[J]. 浙江农业学报, 2021, 33(9): 1602-1610.

SHEN Liuhong, CHENG Lijie, YOU Liuchao, YONG Kang, LUO Zhengzhong, CHEN Jiubing, LUO Qiao, YU Shumin, CAO Suizhong. Effects of heat stress and spray-fan cooling system on physiology and production efficiency of dairy cows in different lactation stages[J]. Acta Agriculturae Zhejiangensis, 2021, 33(9): 1602-1610.

图/表 8

参考文献 36

[1]	韩佳良, 刘建新, 刘红云. 热应激对奶牛泌乳性能的影响及其机制[J]. 中国农业科学, 2018, 51(16):3162-3170.
	HAN J L, LIU J X, LIU H Y. Effect of heat stress on lactation performance in dairy cows[J]. Scientia Agricultura Sinica, 2018, 51(16):3162-3170.(in Chinese with English abstract)
[2]	SCHÄR C, VIDALE P L, LÜTHI D, et al. The role of increasing temperature variability in European summer heatwaves[J]. Nature, 2004, 427(6972):332-336. DOI URL
[3]	王祖新, 王之盛, 王立志, 等. 不同季节温湿度指数对奶牛生产性能和生理生化指标的影响[J]. 中国畜牧杂志, 2009, 45(23):60-63.
	WANG Z X, WANG Z S, WANG L Z, et al. Effect of THI of different seasons on performance, physiological and biochemical parameters of cows[J]. Chinese Journal of Animal Science, 2009, 45(23):60-63.(in Chinese)
[4]	GUNN K M, HOLLY M A, VEITH T L, et al. Projected heat stress challenges and abatement opportunities for US milk production[J]. PLoS One, 2019, 14(3):e0214665. DOI URL
[5]	BAUMGARD L H, RHOADS R P. Ruminant nutrition symposium: ruminant production and metabolic responses to heat stress[J]. Journal of Animal Science, 2012, 90(6):1855-1865. DOI URL
[6]	陈波. 奶牛热应激及应对措施[J]. 中国奶牛, 2014(1):56-58.
	CHEN B. Heat stress and countermeasures of cows[J]. China Dairy Cattle, 2014(1):56-58.(in Chinese)
[7]	HAHN G L. Dynamic responses of cattle to thermal heat loads[J]. Journal of Animal Science, 1999, 77(Suppl. 2):10-20. DOI URL
[8]	中华人民共和国农业农村部. 奶牛热应激评价技术规范: NY/T 2363—2013[S]. 北京:: 中国标准出版社, 2013.
[9]	KHONGDEE S, CHAIYABUTR N, HINCH G, et al. Effects of evaporative cooling on reproductive performance and milk production of dairy cows in hot wet conditions[J]. International Journal of Biometeorology, 2006, 50(5):253-257. DOI URL
[10]	GERNAND E, KÖNIG S, KIPP C. Influence of on-farm measurements for heat stress indicators on dairy cow productivity, female fertility, and health[J]. Journal of Dairy Science, 2019, 102(7):6660-6671. DOI URL
[11]	DAHL G E. Impact and mitigation of heat stress for mastitis control[J]. The Veterinary Clinics of North America Food Animal Practice, 2018, 34(3):473-478. DOI URL
[12]	高民, 杜瑞平, 温雅丽. 热应激对奶牛生产的影响及应对策略[J]. 畜牧与饲料科学, 2011, 32(Z1):59-62.
	GAO M, DU R P, WEN Y L. Effects of heat stress on dairy cow production and coping strategies[J]. Animal Husbandry and Feed Science, 2011, 32(Z1):59-62.(in Chinese)
[13]	张瑞华, 张峥臻, 张克春. 上海地区规模奶牛场夏季物理降温模式调查及其效果测定[J]. 中国奶牛, 2015(15):44-47.
	ZHANG R H, ZHANG Z Z, ZHANG K C. Investigatian of the types and effects detection about physical cooling measures on Shanghai dairy farms[J]. China Dairy Cattle, 2015(15):44-47.(in Chinese with English abstract)
[14]	张健, 蒋永清, 邵涛. 奶牛热应激机理及其营养调控研究进展[J]. 畜牧与兽医, 2009, 41(2):88-92.
	ZHANG J, JIANG Y Q, SHAO T. Research progress on heat stress mechanism and nutrition regulation of cows[J]. Animal Husbandry & Veterinary Medicine, 2009, 41(2):88-92.(in Chinese)
[15]	GAULY M, BOLLWEIN H, BREVES G, et al. Future consequences and challenges for dairy cow production systems arising from climate change in Central Europe: a review[J]. Animal, 2013, 7(5):843-859. DOI URL
[16]	李建国, 桑润滋, 张正珊, 等. 热应激对中国荷斯坦牛血液生化指标及产奶性能的影响[J]. 中国畜牧杂志, 1999, 35(2):25-26.
	LI J G, SANG R Z, ZHANG Z S, et al. Effects of heat stress on blood biochemical indexes and milk production performance of Holstein Cattle in China[J]. Chinese Journal of Animal Science, 1999, 35(2):25-26.(in Chinese)
[17]	廖迎新, 刘德武, 李耀坤, 等. 华南地区不同耐热性荷斯坦奶牛血液生化指标比较[J]. 黑龙江畜牧兽医, 2018(3):108-111.
	LIAO Y X, LIU D W, LI Y K, et al. Comparison of blood biochemical indexes of Holstein cows with different heat tolerance in South China[J]. Heilongjiang Animal Science and Veterinary Medicine, 2018(3):108-111.(in Chinese)
[18]	SCHÜLLER L K, BURFEIND O, HEUWIESER W. Short communication: comparison of ambient temperature, relative humidity, and temperature-humidity index between on-farm measurements and official meteorological data[J]. Journal of Dairy Science, 2013, 96(12):7731-7738. DOI URL
[19]	KADZERE C T, MURPHY M R, SILANIKOVE N, et al. Heat stress in lactating dairy cows: a review[J]. Livestock Production Science, 2002, 77(1):59-91. DOI URL
[20]	ARMSTRONG D V. Heat stress interaction with shade and cooling[J]. Journal of Dairy Science, 1994, 77(7):2044-2050. DOI URL
[21]	傅春泉, 徐苏凌, 张建中. 湿帘冷风机降温在南方奶牛舍中的应用[J]. 中国奶牛, 2011(7):54-56.
	FU C Q, XU S L, ZHANG J Z. Application of wet curtain cooling fan in dairy farm in south China[J]. China Dairy Cattle, 2011(7):54-56.(in Chinese)
[22]	蔡景义, 冯堂超, 廖阔遥, 等. 封闭型牛舍风机喷淋降温和饲粮添加铬改善肉牛生长性能[J]. 农业工程学报, 2015, 31(19):190-194.
	CAI J Y, FENG T C, LIAO K Y, et al. Cooling with fans and spray in closed cowshed and chromium supplementation improving growth performance of beef cattle[J]. Transactions of the Chinese Society of Agricultural Engineering, 2015, 31(19):190-194.(in Chinese with English abstract)
[23]	徐伟, 赵玉超, 曹露, 等. 北京地区夏季奶牛直肠温度及其对产奶量影响初探[J]. 畜牧兽医学报, 2016, 47(4):745-751.
	XU W, ZHAO Y C, CAO L, et al. Study on the effect of dairy cows’ rectal temperature on milk yield in summer in Beijing area[J]. Chinese Journal of Animal and Veterinary Sciences, 2016, 47(4):745-751.(in Chinese with English abstract)
[24]	杨加梅, 梁国飞. 荷斯坦奶牛热应激及耐热性指标的评价[J]. 中国奶牛, 2008(4):23-24.
	YANG J M, LIANG G F. Evaluation of heat stress and heat resistance of Holstein cows[J]. China Dairy Cattle, 2008(4):23-24.(in Chinese)
[25]	李蓉. 高温高湿对泌乳奶牛生产性能和粪样菌群的影响及喷淋效果研究[D]. 武汉: 华中农业大学, 2018.
	LI R. Effects of high temperature-humidity and spray measures on production performance and fecal bacterial communities in lactating cows[D]. Wuhan: Huazhong Agricultural University, 2018. (in Chinese with English abstract)
[26]	WEST J W, MULLINIX B G, BERNARD J K. Effects of hot, humid weather on milk temperature, dry matter intake, and milk yield of lactating dairy cows[J]. Journal of Dairy Science, 2003, 86(1):232-242. DOI URL
[27]	RHOADS M L, RHOADS R P, VANBAALE M J, et al. Effects of heat stress and plane of nutrition on lactating Holstein cows: I. Production, metabolism, and aspects of circulating somatotropin[J]. Journal of Dairy Science, 2009, 92(5):1986-1997. DOI URL
[28]	KLEINJAN-ELAZARY A, BEN-MEIR Y, GACITUA H, et al. Cooling management effects on dry matter intake, metabolic hormones levels and welfare parameters in dairy cows during heat stress[J]. Journal of Dairy Research, 2020, 87(1):64-69. DOI URL
[29]	杨丰利, 李小杉. 热应激对不同泌乳阶段奶牛日产奶量的影响[J]. 湖北农业科学, 2014, 53(19):4650-4653.
	YANG F L, LI X S. Effects of heat stress on daily milk production of dairy cows in different lactation periods[J]. Hubei Agricultural Sciences, 2014, 53(19):4650-4653.(in Chinese with English abstract)
[30]	SPIERS D E, SPAIN J N, SAMPSON J D, et al. Use of physiological parameters to predict milk yield and feed intake in heat-stressed dairy cows[J]. Journal of Thermal Biology, 2004, 29(7/8):759-764. DOI URL
[31]	PURWANTO B P, ABO Y, SAKAMOTO R, et al. Diurnal patterns of heat production and heart rate under thermoneutral conditions in Holstein Friesian cows differing in milk production[J]. The Journal of Agricultural Science, 1990, 114(2):139-142. DOI URL
[32]	李建国, 桑润滋, 张正珊, 等. 热应激对奶牛生理常值、血液生化指标、繁殖及泌乳性能的影响[J]. 河北农业大学学报, 1998, 21(4):69-75.
	LI J G, SANG R Z, ZHANG Z S, et al. Influence of heat stress on physiological response, blood biochemical parameters, reproduction and milk performance of Holstein cow[J]. Journal of Agricultural University of Hebei, 1998, 21(4):69-75.(in Chinese with English abstract)
[33]	MCDOWELL R E, HOOVEN N W, CAMOENS J K. Effect of climate on performance of holsteins in first lactation[J]. Journal of Dairy Science, 1976, 59(5):965-971. DOI URL
[34]	梁学武, 张龙涛, 刘庆华, 等. 热应激期日粮添加酵母铬对奶牛产奶性能及血液生化指标的影响[J]. 福建农林大学学报(自然科学版), 2006, 35(2):191-194.
	LIANG X W, ZHANG L T, LIU Q H, et al. Effects of dietary supplementation of chromium yeast on the performance and blood biochemical parameters in heat-stressed dairy cows[J]. Journal of Fujian Agriculture and Forestry University (Natural Science Edition), 2006, 35(2):191-194.(in Chinese with English abstract)
[35]	FOURNEL S, OUELLET V, CHARBONNEAU É. Practices for alleviating heat stress of dairy cows in humid continental climates: a literature review[J]. Animals, 2017, 7(5):37. DOI URL
[36]	BAVA L, RAPETTI L, CROVETTO G M, et al. Effects of a nonforage diet on milk production, energy, and nitrogen metabolism in dairy goats throughout lactation[J]. Journal of Dairy Science, 2001, 84(11):2450-2459. DOI URL

试验时期 Trial periods	θ/℃	湿度 Humidity/%	风速 Wind speed/(m·s^-1)	温湿指数 THI
热应激期舍内In the bari during heat stress period	29.9±1.87 a	82.2±7.61 A	1.35±0.12 A	82.7±2.16 a
热应激期舍外Outside the barn during heat stress period	30.0±2.18 a	74.9±7.38 B	0.40±0.29 B	81.2±2.48 b
非热应激期舍内In the bari during non-heat stress period	11.9±1.44	80.7±5.84	0.22±0.06	52.2±2.52

试验时期 Trial periods	θ/℃	湿度 Humidity/%	风速 Wind speed/(m·s^-1)	温湿指数 THI
热应激期舍内In the bari during heat stress period	29.9±1.87 a	82.2±7.61 A	1.35±0.12 A	82.7±2.16 a
热应激期舍外Outside the barn during heat stress period	30.0±2.18 a	74.9±7.38 B	0.40±0.29 B	81.2±2.48 b
非热应激期舍内In the bari during non-heat stress period	11.9±1.44	80.7±5.84	0.22±0.06	52.2±2.52

指标 Index	试验时期 Trial period	新产牛 Late perinatal cows	泌乳前期奶牛 Early lactation cows	泌乳中期奶牛 Mid-lactation cows	泌乳后期奶牛 Late lactation cows	平均值 Average value
直肠温度 Rectal temperature/℃	热应激期 Heat stress period	39.8±0.26 A	39.5±0.33 A	39.3±0.20 A	39.2±0.21 A	39.4±0.35 A
	非热应激期 Non-heat stress period	38.9±0.05 B	38.8±0.06 B	38.7±0.04 B	38.7±0.04 B	38.8±0.08 B
呼吸频率	热应激期	68.1±9.04 a	63.7±9.04 a	63.0±8.57 a	62.3±8.75 a	64.3±9.09 a
Respiration rate/	Heat stress period
(time·min^-1)	非热应激期	35.2±2.04 b	34.6±1.70 b	33.7±1.51 b	33.8±1.32 b	34.5±1.88 b
	Non-heat stress period

指标 Index	试验时期 Trial period	新产牛 Late perinatal cows	泌乳前期奶牛 Early lactation cows	泌乳中期奶牛 Mid-lactation cows	泌乳后期奶牛 Late lactation cows	平均值 Average value
直肠温度 Rectal temperature/℃	热应激期 Heat stress period	39.8±0.26 A	39.5±0.33 A	39.3±0.20 A	39.2±0.21 A	39.4±0.35 A
	非热应激期 Non-heat stress period	38.9±0.05 B	38.8±0.06 B	38.7±0.04 B	38.7±0.04 B	38.8±0.08 B
呼吸频率	热应激期	68.1±9.04 a	63.7±9.04 a	63.0±8.57 a	62.3±8.75 a	64.3±9.09 a
Respiration rate/	Heat stress period
(time·min^-1)	非热应激期	35.2±2.04 b	34.6±1.70 b	33.7±1.51 b	33.8±1.32 b	34.5±1.88 b
	Non-heat stress period

指标 Index	试验时期 Trial period	新产牛 Late perinatal cows	泌乳前期奶牛 Early lactation cows	泌乳中期奶牛 Mid-lactation cows	泌乳后期奶牛 Late lactation cows	平均值 Average value
日均单产	热应激期	26.6±3.41 B	27.9±1.12 B	27.4±1.41 B	18.6±1.32 a	25.1±0.95 B
Average daily	Heat stress period
milk yield/kg	非热应激期	29.2±2.28 A	34.5±0.95 A	31.6±1.95 A	18.0±1.35 a	28.3±0.78 A
	Non-heat stress period
平均泌乳时间	热应激期	12.3±1.14 a	84.3±6.51 a	147.3±5.27 a	253.8±5.17 a	126.8±89.92 a
Average time in milk/d	Heat stress period
	非热应激期	12.3±0.92 a	82.6±8.88 a	149.0±9.13 a	254.5±9.89 a	124.4±90.70 a
	Non-heat stress period

热应激与喷淋-风扇系统对不同泌乳阶段奶牛生理和生产性能的影响

Effects of heat stress and spray-fan cooling system on physiology and production efficiency of dairy cows in different lactation stages

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 8

参考文献 36

相关文章 15

编辑推荐

Metrics

本文评价

试验时期 Trial period	乳脂率 Milk fat percentage/%	乳蛋白 Milk protein percentage/%	脂蛋比 Milk fat/protein	体细胞数 Somatic cell count/ (10⁴ mL^-1)
热应激期Heat stress period	4.00±0.07 B	3.36±0.06 b	1.19±0.25 B	26.7±4.80 a
非热应激期Non-heat stress period	4.29±0.10 A	3.42±0.03 a	1.26±0.04 A	25.5±3.47 a

[1]	赵洪喜, 刘继兵. 宁夏部分地区奶牛球虫感染情况调查与遗传进化分析[J]. 浙江农业学报, 2021, 33(8): 1379-1384.
[2]	沈留红, 钱柏霖, 尤留超, 张钺, 莘余, 吕尚揆, 肖劲邦, 余树民, 苏柘僮, 董可, 杨世林, 冯育林, 曹随忠. 白头翁皂苷B4对临床型奶牛乳房炎疗效和血清炎性因子、免疫因子的影响[J]. 浙江农业学报, 2021, 33(7): 1184-1191.
[3]	吴佳, 陈朗, 姜涛, 黄国明, 李倬, 李耀东, 张丽, 刘丽霞. 奶牛CSF3基因遗传多态性筛查及其生物信息学分析[J]. 浙江农业学报, 2020, 32(6): 986-993.
[4]	李秋玲, 齐颖, 王琛, 张一名, 王新妤, 尚校兰, 贾永红, 李美茹, 储明星. 热应激对中国荷斯坦牛乳腺组织基因表达及信号通路的影响[J]. 浙江农业学报, 2020, 32(5): 770-778.
[5]	曾学琴, 柳陈坚, 杨雪, 李晓然. 高通量测序法检测奶牛乳房炎关联微生物群落结构及多样性[J]. 浙江农业学报, 2019, 31(9): 1437-1445.
[6]	朱颍琨, 肖劲邦, 钱柏霖, 姜思汛, 尤留超, 张钺, 刘红, 马莉, 曹随忠, 余树民, 沈留红. 泌乳初期奶牛相关脂肪因子及生理生化指标与脂肪肝的相关性[J]. 浙江农业学报, 2019, 31(5): 722-729.
[7]	白东东, 李新圃, 杨峰, 罗金印, 王旭荣, 李宏胜. 地肤通乳散治疗奶牛乳房炎临床疗效及作用靶点预测[J]. 浙江农业学报, 2019, 31(5): 730-736.
[8]	徐晓锋, 郭成. 淀粉诱导奶牛乳脂下降后奶牛瘤胃细菌菌群变化[J]. 浙江农业学报, 2019, 31(10): 1591-1598.
[9]	王宇, 李碧, 阳明贤, 左之才. 奶牛隐性乳房炎三种致病菌的多重PCR检测方法的建立[J]. 浙江农业学报, 2019, 31(1): 20-29.
[10]	贾先波, 杨舒慧, 丁鹏, 陈仕毅, 王杰, 胡深强, 赖松家. 四川丘陵地区中国荷斯坦奶牛围产后期血液生理生化指标分析[J]. 浙江农业学报, 2018, 30(6): 953-960.
[11]	沈留红, 巫晓峰, 肖劲邦, 姜思汛, 邓俊良, 左之才, 傅宏庆, 曹随忠, 余树民, 张有瑞. 回乳期奶牛血清GH、INS、HC、TGF-β1和IGF-1含量变化[J]. 浙江农业学报, 2017, 29(4): 548-554.
[12]	张满囤, 单新媛, 于洋, 米娜, 阎刚, 郭迎春. 基于小波变换和改进KPCA的奶牛个体识别研究[J]. 浙江农业学报, 2017, 29(12): 2000-2008.
[13]	沈留红, 江涛, 肖劲邦, 姜思汛, 巫晓峰, 曹随忠, 余树民, 邓俊良, 彭广能, 左之才. 奶牛静脉血和脐静脉血脂联素、瘦素及IGF-1表达水平与犊牛初生体质量相关性研究[J]. 浙江农业学报, 2017, 29(1): 37-43.
[14]	刘祥, 孔智翔, 殷书平, 殴莎莎, 刘成艳, 同韩虎, 吴三桥, 陈琛, 陈春琳. 奶牛乳房炎重要候选疫苗蛋白的抗原表位分析及三联重组表位疫苗的氨基酸序列设计[J]. 浙江农业学报, 2016, 28(9): 1476-1484.
[15]	刘勇，张领，吴晓庆，卫朝辉，王艳红，王启磾，高迪，丁彪，吴风瑞，王荣，李文雍*. 热应激对猪卵母细胞葡萄糖\\|6\\|磷酸脱氢酶活性及染色质构型的影响[J]. 浙江农业学报, 2016, 28(1): 22-.