不同品种芹菜可食部分的降压活性成分及其体外血管紧张素转换酶抑制活性

doi:10.3969/j.issn.1004-1524.2021.12.22

浙江农业学报 ›› 2021, Vol. 33 ›› Issue (12): 2415-2422.DOI: 10.3969/j.issn.1004-1524.2021.12.22

不同品种芹菜可食部分的降压活性成分及其体外血管紧张素转换酶抑制活性

马子甲¹(), 张凯杰², 陆胜民¹^,³^,^*(), 徐志豪⁴, 郑美瑜³, 刘哲³, 王璐³, 杨颖³

1.浙江师范大学化学与生命科学学院,浙江金华 321000
2.江苏楷益智能科技有限公司,江苏无锡 214100
3.浙江省农业科学院食品科学研究所/浙江省果蔬保鲜与加工技术研究重点实验室,浙江杭州 310021
4.浙江省农业科学院蔬菜研究所,浙江杭州 310021

收稿日期:2021-05-13 出版日期:2021-12-25 发布日期:2022-01-10
通讯作者: 陆胜民
作者简介:* 陆胜民,E-mail: lushengmin@hotmail.com
马子甲(1996—),男,浙江金华人,硕士研究生,研究方向为农产品加工。E-mail: 1317489993@qq.com
基金资助:
浙江省重点研发计划(2017C02004)

Antihypertensive active components in edible parts of different varieties of celeries and their in vitro angiotensin converting enzyme inhibitory activities

MA Zijia¹(), ZHANG Kaijie², LU Shengmin¹^,³^,^*(), XU Zhihao⁴, ZHENG Meiyu³, LIU Zhe³, WANG Lu³, YANG Ying³

1. College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321000, China
2. Jiangsu Kaiyi Intelligent Technology Co., Ltd., Wuxi 214100, China
3. Institute of Food Science/Key Laboratory of Fruit and Vegetables Postharvest and Processing Technology Research of Zhejiang Province, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China
4. Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2021-05-13 Online:2021-12-25 Published:2022-01-10
Contact: LU Shengmin

摘要/Abstract

摘要：

以青梗芹菜、白芹菜、紫芹等19个品种芹菜为研究对象,在相同条件[料液比1∶30 (g·mL^-1)、时间2 h、温度60 ℃]下提取得到可食用部分(茎和叶)的水提取液,用于筛选具较高体外血管紧张素转换酶(ACE)抑制活性、总酚和总黄酮含量的芹菜品种及其部位。结果表明,芹菜叶水提物的体外ACE抑制活性显著(P<0.05)高于乙醇溶液提取物;不同品种芹菜叶水提物的体外ACE抑制活性集中在80%~100%;总黄酮含量集中在200~250 mg·L^-1,白梗芹菜叶中含量最高,白芹叶中含量最低;总酚含量集中在130~150 mg·L^-1,紫芹叶中含量最高,空杆绿芹叶中含量最低。相关性分析表明,芹菜叶的体外ACE抑制活性与其总酚和总黄酮含量均呈正相关,其相关系数均在0.3~0.6。体外ACE半抑制浓度(IC₅₀)表明,青梗芹菜叶水提物的IC₅₀最低(1.258 mg·mL^-1),其次为四季慢芹菜叶和白芹菜叶,分别为1.368 mg·mL^-1和1.829 mg·mL^-1。

关键词: 芹菜, 品种, 血管紧张素转化酶抑制活性, 总黄酮, 总多酚

Abstract:

Nineteen varieties of celeries, including Green stalk celery, White celery, Purple celery, etc. were taken as research materials. Water extracts of the edible parts (stems and leaves) of the celeries investigated were prepared under the same conditions(1∶30 g·mL^-1in solid-liquid ratio, 2 h in extraction time and 60 ℃ in extraction temperature) to screen out the varieties and their edible parts with higher in vitro angiotensin converting enzyme (ACE) inhibitory activity, total phenols and total flavonoids contents. The results indicated that in vitro ACE inhibitory activities of celery leaves extracted with water were significantly higher than those with ethanol solution (P<0.05). The in vitro ACE inhibitory activities in leaf aqueous extracts of different celery varieties concentrated in 80%-100%; total flavonoids contents were focused on 200-250 mg·L ^-1, with highest in White-stalk celery leaf and lowest in White celery leaf; total polyphenols contents were focused on 130-150 mg·L^-1, with highest in Purple celery leaf and lowest in Hollow stalk green celery. Correlation analysis indicated that in vitro ACE inhibitory activity was positively related with both of total flavonoids content and total polyphenols content, with their correlation coefficients being 0.3-0.6. Green stalk celery leaf had the lowest half inhibitory concentration (IC₅₀) values of ACE (1.258 mg·mL ^-1), followed by Four season celery (1.368 mg·mL^-1) and White celery leaf (1.829 mg·mL^-1).

Key words: celery, varieties, angiotensin converting enzyme inhibitory activity, total flavonoids, total phenolics

中图分类号:

S636.3

马子甲, 张凯杰, 陆胜民, 徐志豪, 郑美瑜, 刘哲, 王璐, 杨颖. 不同品种芹菜可食部分的降压活性成分及其体外血管紧张素转换酶抑制活性[J]. 浙江农业学报, 2021, 33(12): 2415-2422.

MA Zijia, ZHANG Kaijie, LU Shengmin, XU Zhihao, ZHENG Meiyu, LIU Zhe, WANG Lu, YANG Ying. Antihypertensive active components in edible parts of different varieties of celeries and their in vitro angiotensin converting enzyme inhibitory activities[J]. Acta Agriculturae Zhejiangensis, 2021, 33(12): 2415-2422.

图/表 5

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编号 No.	品种名称 Celery varieties	体外ACE抑制活性 ACE inhibitory activity/%	总黄酮含量 Total flavonoids content/(mg·L^-1)	总酚含量 Total phenolics content/(mg·L^-1)
1	四季慢芹菜Four seasoncelery	92.80±1.80 ef	224.23±1.35 g	170.45±1.46 b
2	紫芹Purple celery	105.13±1.41 a	239.55±3.96 d	182.49±3.65 a
3	白芹White celery	88.23±1.58 h	168.38±0.88 m	131.24±1.55 i
4	实杆芹Solid stalk celery	95.08±0.80 de	208.56±2.26 i	139.50±1.71 fgh
5	新云芹菜Xinyun celery	89.48±3.18 gh	181.89±1.32 l	128.56±0.86 i
6	白药芹Whiteherb celery	91.98±1.27 fg	242.25±5.12 d	139.50±1.55 fgh
7	铁杆青Tieganqing	89.92±1.15 gh	198.11±0.88 j	140.70±0.99 fg
8	泾川芹菜Jingchuan celery	79.79±1.02 i	253.24±3.97 b	143.08±2.18 f
9	空杆绿芹Hollow stalk greencelery	98.22±1.05 c	188.20±0.51 k	119.40±1.29 j
10	蒲芹Pu celery	94.27±1.00 ef	237.93±1.55 de	138.91±1.01 gh
11	白梗芹菜Whitestalk celery	98.24±0.98 bc	261.71±2.43 a	151.94±2.81 d
12	青梗芹菜Green stalk celery	103.86±1.62 a	261.35±0.44 a	160.80±0.61 c
13	重庆白芹菜Chongqing white celery	94.08±0.35 ef	235.05±4.44 ef	140.30±1.76 fg
14	凤凰芹菜Phoenix celery	96.78±1.04 cd	199.19±0.88 j	135.92±1.01 h
15	细叶芹菜Fine leafcelery	104.11±2.17 a	247.66±1.42 c	140.20±2.74 fg
16	青药芹Green herb celery	101.16±3.28 b	251.08±0.44 bc	141.79±0.84 fg
17	多伦实干芹菜Duolunsolid celery	97.95±1.36 cd	231.80±3.43 f	153.13±4.10 d
18	Ts123.010	96.81±0.46 cd	247.30±2.34 c	161.69±1.20 c
19	大芹菜Bigcelery	93.42±0.62 ef	218.11±1.92 h	147.26±2.20 e

编号 No.	品种名称 Celery varieties	体外ACE抑制活性 ACE inhibitory activity/%	总黄酮含量 Total flavonoids content/(mg·L^-1)	总酚含量 Total phenolics content/(mg·L^-1)
1	四季慢芹菜Four seasoncelery	92.80±1.80 ef	224.23±1.35 g	170.45±1.46 b
2	紫芹Purple celery	105.13±1.41 a	239.55±3.96 d	182.49±3.65 a
3	白芹White celery	88.23±1.58 h	168.38±0.88 m	131.24±1.55 i
4	实杆芹Solid stalk celery	95.08±0.80 de	208.56±2.26 i	139.50±1.71 fgh
5	新云芹菜Xinyun celery	89.48±3.18 gh	181.89±1.32 l	128.56±0.86 i
6	白药芹Whiteherb celery	91.98±1.27 fg	242.25±5.12 d	139.50±1.55 fgh
7	铁杆青Tieganqing	89.92±1.15 gh	198.11±0.88 j	140.70±0.99 fg
8	泾川芹菜Jingchuan celery	79.79±1.02 i	253.24±3.97 b	143.08±2.18 f
9	空杆绿芹Hollow stalk greencelery	98.22±1.05 c	188.20±0.51 k	119.40±1.29 j
10	蒲芹Pu celery	94.27±1.00 ef	237.93±1.55 de	138.91±1.01 gh
11	白梗芹菜Whitestalk celery	98.24±0.98 bc	261.71±2.43 a	151.94±2.81 d
12	青梗芹菜Green stalk celery	103.86±1.62 a	261.35±0.44 a	160.80±0.61 c
13	重庆白芹菜Chongqing white celery	94.08±0.35 ef	235.05±4.44 ef	140.30±1.76 fg
14	凤凰芹菜Phoenix celery	96.78±1.04 cd	199.19±0.88 j	135.92±1.01 h
15	细叶芹菜Fine leafcelery	104.11±2.17 a	247.66±1.42 c	140.20±2.74 fg
16	青药芹Green herb celery	101.16±3.28 b	251.08±0.44 bc	141.79±0.84 fg
17	多伦实干芹菜Duolunsolid celery	97.95±1.36 cd	231.80±3.43 f	153.13±4.10 d
18	Ts123.010	96.81±0.46 cd	247.30±2.34 c	161.69±1.20 c
19	大芹菜Bigcelery	93.42±0.62 ef	218.11±1.92 h	147.26±2.20 e

指标 Index	体外ACE抑制活性 In vitro ACE inhibitory activity	总黄酮含量 Total flavonoids content	总酚含量 Total phenolics content
体外ACE抑制活性 In vitro ACE inhibitory activity	1
总黄酮含量Total flavonoids content	0.363	1
总酚含量Total phenolics content	0.378	0.550	1

指标 Index	体外ACE抑制活性 In vitro ACE inhibitory activity	总黄酮含量 Total flavonoids content	总酚含量 Total phenolics content
体外ACE抑制活性 In vitro ACE inhibitory activity	1
总黄酮含量Total flavonoids content	0.363	1
总酚含量Total phenolics content	0.378	0.550	1

不同品种芹菜可食部分的降压活性成分及其体外血管紧张素转换酶抑制活性

Antihypertensive active components in edible parts of different varieties of celeries and their in vitro angiotensin converting enzyme inhibitory activities

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