芍药不同品种发育过程中花茎细胞壁成分变化

doi:10.3969/j.issn.1004-1524.2022.10.16

浙江农业学报 ›› 2022, Vol. 34 ›› Issue (10): 2220-2229.DOI: 10.3969/j.issn.1004-1524.2022.10.16

芍药不同品种发育过程中花茎细胞壁成分变化

侯竞涵¹(), 万映伶¹, 刘爱青², 洪爱英², 刘燕¹^,^*()

1.北京林业大学园林学院,花卉种质创新与分子育种北京市重点实验室,国家花卉工程技术研究中心,城乡生态环境北京实验室,北京100083
2.菏泽市城市管理事业发展中心,山东菏泽 274000

收稿日期:2022-02-09 出版日期:2022-10-25 发布日期:2022-10-26
通讯作者: 刘燕
作者简介:*刘燕,E-mail: chblyan@163.com
侯竞涵(1997—),女,山东威海人,硕士,主要从事芍药茎直立性研究。E-mail: calyces97@163.com
基金资助:
国家自然科学基金(32071825)

Content changes of cell wall composition during stem development in different varieties of Paeonia lactiflora

HOU Jinghan¹(), WAN Yingling¹, LIU Aiqing², HONG Aiying², LIU Yan¹^,^*()

1. Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture, Beijing Laboratory of Urban and Rural Ecological Environment, School of Landscape Architecture, Beijing Forestry University, Beijing 100083, China
2. Urban Management Development Center of Heze, Heze 274000, Shandong, China

Received:2022-02-09 Online:2022-10-25 Published:2022-10-26
Contact: LIU Yan

摘要/Abstract

摘要：

花茎挺直是评判芍药切花品质的重要标准之一,但目前影响芍药花茎直立性的因素尚不完全清楚。为明确直立性不同的芍药品种发育过程中花茎细胞壁成分的变化差异,利用花枝夹角指标将6个品种划分为3个直立等级,将现蕾期到花朵盛开的过程划分成S1~S5共5个阶段,观测不同直立等级品种花茎弯曲发生的关键时期及其5个发育阶段细胞壁组分的动态变化。结果表明,切花品种东方少女和杨妃出浴直立性最好,迟粉等3个品种花茎自S2时期起随发育逐渐变弯,晴雯自S4时期起迅速下垂。比较一致的规律是,3个直立等级的品种木质素含量随发育进程均呈升高趋势,水溶性果胶含量自S2时期起呈下降趋势。相关性分析表明,在发育前期花茎直立性和木质素含量极显著正相关;发育中期花茎直立性和木质素含量极显著正相关,和原果胶含量显著负相关;发育后期花茎直立性和半纤维素含量、木质素含量极显著正相关,和水溶性果胶含量显著负相关。研究为切花芍药品种的选育和栽培调控提供了理论依据。

关键词: 芍药, 茎直立性, 纤维素, 半纤维素, 木质素, 原果胶, 水溶性果胶

Abstract:

Straight stem is one of the vital standards to judge cut peony. However, the factors affecting stem verticality of Paeonia lactiflora are not clear at present. To clarify the changes of cell wall components in stem characteristics of varieties with different straightness, six varieties were studied, and three levels of straightness were divided based on the angle between flower and plumb line. The process from budding to flower blooming was divided into S1 to S5 stages. Then, the key stage of stem bending in varieties with different straightness and dynamic changes of cell wall components in five stages were studied. The results showed that two cut peonies, Dongfang Shaonv and Yangfei Chuyu had relatively most straight stems. Chifen and other two cultivars bent along with stem development from S2 stage, and Qingwen bent rapidly from S4 stage. Similar trends were found in various cultivars that lignin content increased along with development and water-soluble pectin decreased from S2 stage. Correlation analysis showed that there was a significant positive correlation between stem straightness and lignin content at early development stage. The stem straightness was positively correlated with lignin content and negatively correlated with propectin content in the middle stage of development. The stem straightness was positively correlated with hemicellulose content and lignin content, and negatively correlated with water-soluble pectin content. The result would provide a theoretical basis for breeding and cultivation regulation of cut herbaceous peonies.

Key words: Paeonia lactiflora, stem straightness, cellulose, hemicellulose, lignin, protopectin, water-soluble pectin

中图分类号:

S682.12

侯竞涵, 万映伶, 刘爱青, 洪爱英, 刘燕. 芍药不同品种发育过程中花茎细胞壁成分变化[J]. 浙江农业学报, 2022, 34(10): 2220-2229.

HOU Jinghan, WAN Yingling, LIU Aiqing, HONG Aiying, LIU Yan. Content changes of cell wall composition during stem development in different varieties of Paeonia lactiflora[J]. Acta Agriculturae Zhejiangensis, 2022, 34(10): 2220-2229.

图/表 9

图1 芍药样品取材时期 A,S1时期;B,S2时期;C,S3时期;D,S4时期;E,S5时期。

Fig.1 Sampling time of Paeonia lactiflora A, Stage 1 (S1); B, Stage 2 (S2); C, Stage 3 (S3); D, Stage 4 (S4); E, Stage 5 (S5).

图2 芍药花枝夹角测量方式α表示花枝夹角。

Fig.2 Measurement method of flower branch angle of Paeonia lactifloraα represented flower branch angle.

表1 不同芍药品种在5个发育阶段的花枝夹角

Table 1 Flower branch angle of various herbaceous peony cultivars at five developmental stages

品种名 Cultivar name	不同时期的花枝夹角 Flower branch angle at different stages/(°)					直立等级 Vertical grade
品种名 Cultivar name	S1	S2	S3	S4	S5	直立等级 Vertical grade
东方少女Dongfang Shaonv	3.38±0.73 Ba	5.78±0.65 Da	6.12±0.60 Ea	6.00±1.00 Da	5.95±0.62 Ea	Ⅰ
杨妃出浴Yangfei Chuyu	6.02±0.46 Ab	8.07±1.18 Cab	10.45±1.96 Da	7.30±1.74 Dab	10.53±0.84 Da	Ⅰ
迟粉Chifen	4.80±0.75 ABe	12.72±1.98 Bd	33.15±2.69 Ab	26.80±0.84 Cc	36.58±2.79 Ca	Ⅱ
种生粉Zhongshengfen	5.18±1.38 Ae	10.07±2.13 Cd	24.62±0.98 Bc	37.53±4.61 Bb	41.90±4.29 Ba	Ⅱ
奇花露霜Qihua Lushuang	4.52±0.90 ABd	16.48±2.97 Ac	13.82±2.04 Cc	36.97±3.82 Bb	56.38±4.66 Aa	Ⅲ
晴雯Qingwen	4.53±2.08 ABb	5.10±1.12 Db	5.83±1.08 Eb	54.05±5.15 Aa	54.23±5.12 Aa	Ⅲ

图3 各时期不同直立等级芍药的纤维素含量大写字母为同一时期不同品种间的差异(P<0.05),小写字母为同一品种不同时期的差异(P<0.05)。下同。

Fig.3 Cellulose content of Paeonia lactiflora with different vertical grades at five developmental stages Capital letters refered to the differences (P<0.05) between various varieties in the same stage, and lowercase letters refered to the differences (P<0.05) among different stages of a variety. The same as below.

图4 各时期不同直立等级芍药品种的半纤维素含量

Fig.4 Hemicellulose content of Paeonia lactiflora varieties with different vertical grades at five developmental stages

图5 各时期不同直立等级芍药品种的木质素含量

Fig.5 Lignin content of Paeonia lactiflora with different vertical grades at five developmental stages

图6 各时期不同直立等级芍药品种的原果胶含量

Fig.6 Protopectin content of Paeonia lactiflora with different vertical grades at five developmental stages

图7 各时期不同直立等级芍药品种的水溶性果胶含量

Fig.7 Water-soluble pectin content of Paeonia lactiflora with different vertical grades at five developmental stages

表2 不同品种芍药在5个发育阶段的花枝夹角与细胞壁组分含量间的相关性

Table 2 Correlation analysis between flower branch angle and cell wall component content of various herbaceous peony cultivars at five developmental stages

细胞壁组分 Cell wall components	发育时期Developmental stages
细胞壁组分 Cell wall components	S1	S2	S3	S4	S5
纤维素Cellulose	0.024	-0.405	-0.503	0.039	-0.090
半纤维素Hemicellulose	0.001	-0.210	-0.057	-0.604^**	0.024
木质素Lignin	-0.053	-0.761^**	-0.743^**	-0.419	-0.678^**
原果胶Protopectin	-0.379	-0.242	0.565^*	0.159	0.069
水溶性果胶Water-soluble pectin	-0.100	0.266	0.058	0.358	0.864^*

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芍药不同品种发育过程中花茎细胞壁成分变化

Content changes of cell wall composition during stem development in different varieties of Paeonia lactiflora

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