›› 2010, Vol. 22 ›› Issue (5): 576-579.

• 论文 • 上一篇    下一篇

利用SRAP技术分析茭白种质资源遗传多样性

丁潮洪1,华金渭1,胡婷婷2, 徐沛2   

  1. 1丽水市农业科学研究院,浙江 丽水323000;2浙江省农业科学院蔬菜研究所,浙江 杭州 310021
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-09-25 发布日期:2010-09-25

Genetic diversity analysis of water bamboo (Zizania latifolia Turcz.) germplasms in Zhejiang employing SRAP markers

DING Chao-hong;HUA Jin-wei;HU Ting-ting;XU Pei   

  1. 1 Lishui Academy of Agricultural Sciences, Lishui 323000, China; 2 Institute of Vegetable,Zhejiang Academy Agricultural Sciences, Hangzhou 310021, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-09-25 Published:2010-09-25

摘要: 采用简化的SRAP分析方法,用47对SRAP引物对35份茭白材料进行遗传多样性分析,共获得153个扩增位点,其中多态性位点11个,平均多态水平为7.2%,扩增产物的片段大小范围在50~400 bp。利用UPGMA法进行聚类分析,以遗传相似系数0.51为阈值,将35个茭白品系分为2个类群。单双季茭间遗传距离大于各类型内部差异,来源地相同的材料具有更高的遗传相似性。

关键词: 茭白, SRAP, 遗传多样性, 聚类分析

Abstract: The genetic diversity analysis among 35 accessions of water bamboo (Zizania latifolia Turcz.) from Zhejiang Province was conducted using 47 pairs of SRAP primers. A total of 153 loci were detected, 11 being polymorphic, which equalled to an average polymorphism rate of 7.2%. The size of amplified PCR products ranged from 50-400 bp. At a threshold of 0.51, these accessions were generally clustered into two groups. Genetic distance between single-cropping and double-cropping water bamboo was greater than the distance within each type, while the accessions with closer geographic origins showed higher genetic similarity.

Key words: water bamboo, SRAP, genetic diversity, cluster analysis