浙江农业学报

• 植物保护 • 上一篇    下一篇

假臭草精油对小菜蛾的毒力及酶活力的影响

  

  1. (龙岩市农业科学研究所,福建 龙岩364000)
  • 出版日期:2014-09-25 发布日期:2014-10-11

Effects of essential oil extracted from Praxelis clematidea on enzyme activities of Plutella xylostella and its toxicity

  1. (Laboratory of Plant Protection, Longyan Institute of Agricultural Sciences, Fujian 364000, China)
  • Online:2014-09-25 Published:2014-10-11

摘要: 为了探讨外来入侵杂草假臭草的科学利用方法,用点滴法和熏蒸法测定了假臭草精油对小菜蛾的毒杀、拒食作用及其对小菜蛾体内羧酸酯酶和谷胱甘肽\|s\|转移酶活性的影响。结果表明,假臭草精油对小菜蛾幼虫有一定的毒杀作用,点滴处理48 h致死中浓度为2102×105 mg·L-1、熏蒸处理48 h致死中浓度为1404×105 mg·L-1;假臭草精油对小菜蛾幼虫有明显的拒食作用,12 h拒食中浓度为269×104 mg·L-1,处理浓度为100×105 mg·L-1,拒食率可达100%,不同处理时间的拒食中浓度无明显差异。假臭草精油处理小菜蛾幼虫会导致小菜蛾幼虫体内的羧酸酯酶和谷胱甘肽\|s\|转移酶活性的变化,但未呈现出规律性。

关键词: 假臭草精油, 小菜蛾, 毒力, 羧酸酯酶, 谷胱甘肽\, s\, 转移酶活性

Abstract: To investigate the new methods and tools of alien weeds usage, effects of essential oil extracted from Praxelis clematidea on enzyme activities (carboxylesterase and glutathione\|s\|transferase) of diamondback moth (DBM), Plutella xylostella, and its toxicity by using topical methods of application and fumigation were studied in this paper. Results showed that LC50 after 48 h of P. clematidea essential oil against DBM larvae was 2102×105 mg·L-1 by topical application methed and was 1404×105 mg·L-1 by fumigation treatment. The median antifeedant concentration (AFC50) of P. clematidea essential oil against DBM larvae was 269×104 mg·L-1. The antifeedant rate could reach 100% when treating concentration was 100×105 mg·L-1. Treating time had no significant effects on AFC50. The activities of carboxylesterase and glutathione\|s\|transferase of DBM larvae treated with P. clematidea essential oil were changed significantly but not in a regular pattern.

Key words: Praxelis clematidea essential oil, diamondback moth, toxicity, carboxylesterase, glutathione\, s\, transferase