不同羽色斑嘴野鸭毛囊中Tyr，Tyrp1及C\|kit基因的表达及调控分析

浙江农业学报

不同羽色斑嘴野鸭毛囊中Tyr，Tyrp1及C\|kit基因的表达及调控分析

（1浙江省农业科学院畜牧兽医研究所，浙江杭州310021；2慈溪市畜牧兽医站，浙江慈溪 315300）

出版日期:2015-05-25 发布日期:2015-05-19

Expression and regulation of Tyr, Tyrp1 and C\|kit gene in feather bulbs of spot\|billed ducks

(1 Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; 2 Cixi Animal Husbandry and Veterinary Station, Cixi 315300, China)

Online:2015-05-25 Published:2015-05-19

摘要/Abstract

摘要： 斑嘴野鸭羽毛呈褐色，少数个体会呈现出黄白羽及白羽表型。Tyrosinase（Tyr）、Tyrosinase\|related protein\|1（Tyrp1）以及C\|kit（The tyrosine kinase receptor）基因与黑色素合成密切相关，研究利用Real\|time PCR对这3个基因在褐羽、黄白羽以及白羽3种表型斑嘴野鸭毛囊组织中进行表达分析；同时利用在线软件分别预测调控这3个基因的microRNA。结果发现，Tyr，Tyrp1在褐羽斑嘴野鸭中表达量最高，黄白羽中表达量显著下降（P＜001），而在白羽中则完全不表达。C\|kit在褐羽及黄白羽中表达量类似，而在白羽中表达则显著下降（P＜001）。同时，预测到调控Tyr，Tyrp1和C\|kit基因的microRNA数分别为9，25和18个。结果表明，毛囊组织中Tyr，Tyrp1以及C\|kit基因的表达下调可能导致了斑嘴野鸭出现了黄白羽及白羽两种表型，这些候选microRNA可能参与了这3个基因的表达调控。

关键词: 斑嘴野鸭, 毛囊, Tyr, Tyrp1, C\, kit, microRNA

Abstract: The feathers of wild spot\|billed ducks are brown, but a few individuals with yellow\|white or white plumage were found when they are domesticated. Tyrosinase (Tyr), Tyrosinase\|related protein\|1 (Tyrp1) and The tyrosine kinase receptor (C\|kit) are genes associated with melanogenesis. In this study, the relative expressions of these genes were analyzed in the three types of feather bulbs using Real\|time PCR, and microRNAs were predicted for these genes. The results showed that Tyr and Tyrp1 were strongly expressed in brown feather bulbs, weakly expressed in yellow\|white feather bulbs, and not expressed in white feather bulbs. C\|kit showed same expression level in brown feather bulbs and yellow\|white bulbs, but not expressed in white feather bulbs. In addition, 9, 25 and 18 microRNAs were predicted to regulate the expression of Tyr, Tyrp1 and C\|kit, respectively. These results suggested that the down regulation of Tyr, Tyrp1 and C\|kit might induce yellow\|white or white plumage of spot\|billed ducks.

Key words: spot\, billed ducks, feather bulbs, Tyr, Tyrp1, C\, kit, microRNA

陈黎1，沈军达1，李国勤1，范益飞2，马建胜2，卢立志1，*. 不同羽色斑嘴野鸭毛囊中Tyr，Tyrp1及C\|kit基因的表达及调控分析[J]. 浙江农业学报.

[1]	邵嘉皓, 王杰, 贾先波, 陈仕毅, 赖松家. 家兔中microRNA的研究进展[J]. 浙江农业学报, 2020, 32(1): 176-182.
[2]	张春荣，何红梅，徐玲英，朱亚红，赵华，吴珉，李振*. 噻虫胺在水稻中的残留分析方法及其消解动态[J]. 浙江农业学报, 2014, 26(5): 1263-.
[3]	舒琦艳;卢立志;傅衍;王得前;原爱平;沈军达;陶争荣;李国勤. 浙江省主要野鸭品种(系)生产性能及肉质性状比较分析[J]. , 2006, 18(4): 0-237.