烤烟烟叶烘烤中蛋白质的降解动态变化规律研究

doi:10.3969/j.issn.1004-1524.2017.12.22

浙江农业学报 ›› 2017, Vol. 29 ›› Issue (12): 2120-2127.DOI: 10.3969/j.issn.1004-1524.2017.12.22

烤烟烟叶烘烤中蛋白质的降解动态变化规律研究

王万能¹, 项钢燎¹, 翟羽晨¹, 马扩彦², 戴亚², 谭兰兰³

1.重庆理工大学药学与生物工程学院,重庆 400054;
2.重庆中烟工业有限责任公司,重庆 400060;
3.四川中烟工业有限责任公司,四川成都 610000

收稿日期:2017-06-06 出版日期:2017-12-20 发布日期:2018-01-08
作者简介:王万能(1971—),男,四川开江人,博士,教授,主要从事生物代谢工程方面的研究。E-mail:wannengw@cqut.edu.cn
基金资助:
重庆中烟工业有限责任公司科技项目(2014Q116)

Dynamic variation rules of protein degradation in flue-cured tobaccos

WANG Wanneng¹, XIANG Gangliao¹, ZHAI Yuchen¹, MA Kuoyan², DAI Ya², TAN Lanlan³

1.College of Pharmacy and Biotechnology, Chongqing University of Technology, Chongqing 400054, China;
2. Chongqing China Tobacco Industry Co., Ltd., Chongqing 400060, China;
3. Sichuan China Tobacco Industry Co., Ltd., Chengdu 610000, China

Received:2017-06-06 Online:2017-12-20 Published:2018-01-08

摘要/Abstract

摘要： 为明确不同部位及不同烘烤时间段烟叶中蛋白质变化规律,利用SDS-PAGE电泳等实验技术,分析烟叶蛋白动态分布和变化特点。烟叶蛋白在不同的烘烤时间段的降解速度不同,在烘烤0~50 h烟叶蛋白质含量的降解速度快,从19%降至9%,烘烤后期趋于平缓。烟叶蛋白分子量分布在11.8~90.5 ku,上、中、下部叶片的蛋白存在明显差异,上部叶片中大、小分子量蛋白含量比中、下部叶片多,下部叶片中分子量蛋白含量较高。不同类别的蛋白在烟叶烘烤过程的不同阶段中降解规律不相同。

关键词: 烟叶, 蛋白质, SDS-PAGE, 降解

Abstract: To analyze and clarify the regularity of protein change in different flue-curing and different flue-curing time of tobacco leaves, and to provide theoretical basis for improving tobacco leaf quality, the dynamic changes and distribution characteristics of tobacco leaves were analyzed by SDS-PAGE and other experimental techniques. The degradation rate of tobacco protein was different at different flue-curing time, and the content of tobacco protein was decreased from 19% to 9% at 0-50 h after flue-curing, and it tended to be gentle in the later flue-curing phase. The molecular weight distribution of leaf protein was between 11.8 and 90.5 ku, and there were significant differences in the content of tobacco protein in the upper, middle and lower leaves. The content of large and small molecular weight protein in the upper leaves was higher than that in the middle and lower leaves, and the content of middle molecular weight in the lower leaves was higher than the others. The degradation rule of different types of protein in different flue-curing stages of tobacco leaf were different.

Key words: tobacco, protein, SDS-PAGE, degradation

中图分类号:

S572

王万能, 项钢燎, 翟羽晨, 马扩彦, 戴亚, 谭兰兰. 烤烟烟叶烘烤中蛋白质的降解动态变化规律研究[J]. 浙江农业学报, 2017, 29(12): 2120-2127.

WANG Wanneng, XIANG Gangliao, ZHAI Yuchen, MA Kuoyan, DAI Ya, TAN Lanlan. Dynamic variation rules of protein degradation in flue-cured tobaccos[J]. , 2017, 29(12): 2120-2127.

参考文献

[1] MACKAY J,ERIKSEN M P.The tobacco atlas[M].Geneva: World Health Organization,2002.
[2] VANSUYT G, SOUCHE G, STRACZEK A, et al. Flux of proteins released by wild type and feritin over-experssor tobacco plants: effect of phosphorus and iron nutrition[J]. Plant Physiology and Biochemistry ,2003,41:27-33.
[3] 张柳,王铮,张亚婕,等.烟草叶片衰老期过程中的蛋白质组学分析[J].植物生理学报,2014, 50 (4): 488-500.
ZHANG L, WANG Z, ZHANG Y J, et al. Proteomic analysis of senescing leaf of tobacco[J]. Plant Physiology Journal , 2014, 50 (4): 488-500. (in Chinese with English abstract)
[4] 崔红,冀浩,张华,等.不同生态区烟草叶片蛋白质组学的比较[J]. 生态学报,2008,28(10): 4873-4880.
CUI H, JI H, ZHANG H, et al. Comparative analysis of leaf proteomes between tobacco plants growing in different ecological regions of China[J]. Acta Ecologicalca Sinica ,2008,28(10): 4873-4880. (in Chinese with English abstract)
[5] 姚光明.降低烟叶中蛋白质含量的研究[J].烟草科技,2000 (9):6-8.
YAO G M.Study on reducing the content of protein in tobacco[J]. Tobacco Science & Technology , 2000 (9):6-8.(in Chinese with English abstract)
[6] 王卫峰,陈江华,宋朝鹏,等.密集烤房的研究进展[J].中国烟草科学,2005,26(3):12-14.
WANG W F, CHEN J H, SONG C P, et al. The research progress on bulk curing barn[J]. Chinese Tobacco Science , 2005,26(3):12-14. (in Chinese with English abstract)
[7] 孙敬权,任四海,吴永德.烤烟燃煤密集烤房的改进探讨[J].烟草科技,2004 (9):43-44.
SUN J Q, REN S H, WU Y D. Improvement of bulk curing barn[J]. Tobacco Science & Technology , 2004 (9):43-44. (in Chinese with English abstract)
[8] 李梅云,高家合,王革,等.微生物对烟叶蛋白质含量的影响[J].生物技术通报,2006(增刊1):376-380.
LI M Y, GAO J H, WANG G, et al. Influence of 3 bacteria isolates on protein content of tobacco leaf[J]. Biotechnology Bulletin , 2006(S1):376-380. (in Chinese with English abstract)
[9] 李常军,宫长荣,李锐,等.烘烤过程中烟叶蛋白质与硝态氮代谢规律研究[J].河南农业大学学报,2000,34(1):47-49.
LI C J, GONG C R, LI R, et al. Studies on the metabolism of protein and nitrate nitrogen in tobacco leaf during curing[J]. Journal of Henan Agricultural University , 2000,34(1):47-49. (in Chinese with English abstract)
[10] 李常军,宫长荣,周义和,等.烤烟烘烤过程中变黄温度对氮素代谢的影响[J].中国烟草学报,2001(2):31-35.
LI C J, GONG C R, ZHOU Y H, et al. The effect of temperature in yellowing stage on nitrogen metabolism during tobacco leaf curing process[J]. Acta Tabacaria Sinica ,2001(2):31-35. (in Chinese with English abstract)
[11] 王爱华,徐秀红,王松峰,等.变黄温度对烤烟烘烤过程中生理指标及烤后质量的影响[J].中国烟草学报,2008,14(1):27-31.
WANG A H, XU X H, WANG S F, et al. Effect of temperature in yellowing stage on physiological indices and quality of flue-cured tobacco[J]. Acta Tabacaria Sinica ,2008,14(1):27-31. (in Chinese with English abstract)
[12] 王允白,王宝华,郭承芳,等.影响烤烟评吸质量的主要化学成分研究[J].中国农业科学,1998,31(1):89-91.
WANG Y B, WANG B H, GUO C F, et al. Study on the main chemical components related to smoking quality in flue-curred tobacco[J]. Scientia Aguricultura Sinica ,1998,31(1):89-91. (in Chinese with English abstract)
[13] 赵谋明, 饶国华,林伟锋.烟草蛋白质研究进展[J].烟草科技,2005(4):31-34.
ZHAO M M, RAO G H, LIN W F. Advance in tobacco protein[J]. Tobacco Science & Technology , 2005(4):31-34. (in Chinese with English abstract)
[14] 管方方,卢伟京, 许旭,等. 大豆特征蛋白的SDS-PAGE研究[J].精细化工,2015,32(4):438-442.
GUAN F F, LU W J, XU X, et al. A study on the characteristic soybean protein by SDS-PAGE[J]. Fine Chemicals , 2015,32(4):438-442. (in Chinese with English abstract)
[15] 杨欣,王秋红,王悦,等.不同地区板蓝根蛋白SDS-PAGE分析[J].中国中医药信息杂志,2015,22(7):86-88.
YANG X, WANG Q H, WANG Y, et al. Analysis on isatidis radix protein in different areas by SDS-PAGE[J]. Chinese Journal of Information on Traditional Chinese Medicine ,2015,22(7):86-88. (in Chinese with English abstract)
[16] 吴林坤,王海斌,尤垂淮,等.地黄块根总蛋白质提取及双向电泳条件优化[J].中国中药杂志,2011,36(8) : 984-987.
WU L K, WANG H B, YOU C H, et al. Establishment of extraction method and 2-dimensional electrophoresis conditions for root tuber proteome analysis of Rehmannia glutinosa [J]. China Journal of Chinese Materia Medica , 2011,36(8) : 984-987. (in Chinese with English abstract)
[17] 李常军,宫长荣,肖鹏,等.施氮水平和烘烤条件对烤后烟叶品质和含氮组分的影响[J].中国烟草科学,2001,22(1):4-7.
LI C J, GONG C R, XIAO P, et al. Influence of nitrogen application and flue-curing condition on nitrogen compounds and tobacco quality[J]. Chinese Tobacco Science , 2001,22(1):4-7. (in Chinese with English abstract)
[18] 许东亚, 孙军伟, 杨惠娟,等.酶抑制剂对烤烟成熟期氮代谢及烤后烟叶品质的影响[J]. 烟草科技,2016(3):17-23.
XU D Y, SUN J W, YANG H J, et al. Inhibitory effects of enzyme on nitrogen metabolism at mature stage and quality of cured tobacco leaves[J]. Tobacco Science&Technology ,2016(3):17-23. (in Chinese with English abstract)
[19] 黄天辉,桂金鹏,郑丽沙.利用酶制剂改善再造烟叶品质研究进展[J].东北农业大学学报,2015,46(10):102-108.
HUANG T H, GUI J P, ZHENG L S. Research advances of reconstituted tobacco sheet quality improved with enzyme preparation[J]. Journal of Northeast Agricultural University , 2015,46(10):102-108. (in Chinese with English abstract)
[20] 张亚恒,王芳,张庆明,等. 微生物在烟叶醇化和再造烟叶生产中的应用进展[J]. 湖北农业科学,2015,54(18):4390-4393.
ZHANG Y H, WANG F, ZHANG Q M, et al. Application of microbiology in flue-cured tobacco leaves during aging and reconstituted production[J]. Hubei Agricultural Sciences ,2015,54(18):4390-4393. (in Chinese with English abstract)
[21] ISAACSON T, DAMASCENO C M, SARAVANAN R S, et al. Sample extraction techniques for enhanced proteomic analysis of plant tissues[J]. Nature Protocols ,2010,1(2):769-774.
[22] RODRIGUES A M, KUBITSCHEK-BARREIRA P H, FERNANDES G F, et al. Two-dimensional gel electrophoresis data for proteomic profiling of Sporothrix yeast cells[J]. Data in Brief , 2015,2:32-38.
[23] 宫长荣.烤烟三段式烘烤导论[M].北京:科学出版社,2006.
[24] 刘勇,叶为民, 王玉胜,等.不同烘烤工艺对云烟87烟叶烘烤质量的影响[J]. 中国烟草科学,2016,37(1):56-60.
LIU Y, YE W M, WANG Y S, et al. Effects of different flue-curing processes on tobacco quality of Yunyan 87[J]. Chinese Tobacco Science , 2016,37(1):56-60. (in Chinese with English abstract)
[25] 朱大恒,韩锦峰,张爱萍,等.自然醇化与人工发酵对烤烟化学成分变化的影响比较研究[J].烟草科技,1999 (1):3-5.
ZHU D H, HAN J F, ZHANG A P, et al.Effects of artificial fermentation and aging on the chemical change in flue-cured tobacco[J]. Tobacco Science & Technology , 1999 (1):3-5.(in Chinese with English abstract)
[26] 李晓,刘凤珠,姚光明,等.酶解法改善烟叶吸味品质的试验[J].烟草科技,2002 (3):14-17.
LI X, LIU F Z, YAO G M, et al.Experiments on improvement of smoking quality of tobacco with enzymatic degradation[J]. Tobacco Science & Technology , 2002 (3):14-17. (in Chinese with English abstract)

烤烟烟叶烘烤中蛋白质的降解动态变化规律研究

Dynamic variation rules of protein degradation in flue-cured tobaccos

RichHTML

PDF (PC)

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	忻晓庭, 刘大群, 张程程, 吴敏, 陈登高, 章检明. 我国特色发酵蔬菜降解亚硝酸盐菌株的筛选鉴定及应用[J]. 浙江农业学报, 2021, 33(2): 335-345.
[2]	许双燕, 张涛, 张成, 林辉, 水贤磊, 郑华宝. 一株红霉素降解菌的筛选、鉴定与降解特性[J]. 浙江农业学报, 2021, 33(1): 131-141.
[3]	高文静, 肖丽娇, 王顺民, 韩秋霞. 降解柴油嗜盐菌的筛选、鉴定及其降解特性[J]. 浙江农业学报, 2020, 32(7): 1241-1252.
[4]	陈乾丽, 汪汉成, 梁永进, 蔡刘体, 黄宇, 周浩, 李忠, 韩洁. 烤后健康烟叶和霉烂烟叶真菌群落结构分析[J]. 浙江农业学报, 2020, 32(6): 1019-1028.
[5]	刘予煊, 程焕, 叶兴乾, 刘慧燕, 方海田. 不同菌株发酵枸杞汁中生物活性物质与香气组成物质含量变化[J]. 浙江农业学报, 2020, 32(3): 499-509.
[6]	裘劼人, 柴伟国, 童建新, 周历萍, 王淑珍. 质谱蛋白质组学研究中草莓雌蕊蛋白提取方法优化[J]. 浙江农业学报, 2020, 32(12): 2186-2191.
[7]	梁浩花, 陶红, 王亚娟, 李娇玲. 一株邻苯二甲酸二丁酯和邻苯二甲酸二(2-乙基己基)酯降解菌的筛选鉴定与降解特性[J]. 浙江农业学报, 2019, 31(7): 1145-1153.
[8]	王哲, 柴里昂, 樊怀福, 杜长霞. 植物响应盐胁迫蛋白质组学研究进展[J]. 浙江农业学报, 2019, 31(6): 1021-1028.
[9]	何开雨, 汤涛, 宣彩迪, 吴园园, 何红梅, 吴珉, 赵学平, 徐霞红, 王强. 氨唑草酮的水解、挥发及其在水-沉积物系统中的降解特性[J]. 浙江农业学报, 2019, 31(12): 2057-2063.
[10]	虞游毅, 杨璐, 廖享, 程平, 武胜利, 李宏. 高效氯氟氰菊酯和氯氰菊酯在苹果中的残留降解动态及其去除方法[J]. 浙江农业学报, 2018, 30(8): 1376-1381.
[11]	李国丽, 曾小英, 翟立翔, 冷艳, 刘梦圆, 李师翁, 陈拓. 一株石油降解菌Lysinibacillus fusiformis 23-1的筛选鉴定及原油降解特性[J]. 浙江农业学报, 2018, 30(7): 1229-1236.
[12]	石江, 赵琳, 朱月清, 楼旭平, 余建忠, 阮松林, 陈文岳. 玉米幼苗叶片响应热胁迫的蛋白质组学分析[J]. 浙江农业学报, 2018, 30(6): 893-908.
[13]	张青, 肖文斐, 裘劼人, 陈初尉, 忻雅, 柴伟国, 阮松林. 免疫诱导剂处理的黑李叶片蛋白质组学分析[J]. 浙江农业学报, 2018, 30(5): 787-796.
[14]	王代懿, 张丰松, 潘娟, 刘登璐, 苟体忠. 水稻秸秆生物炭对雄烯二酮在土壤中吸附与降解行为的影响[J]. 浙江农业学报, 2018, 30(4): 632-639.
[15]	刘紫英, 黄磊, 袁斌, 刘小林, 徐胜光, 黄涛. 一株草莓连作自毒障碍主要物质苯甲酸降解细菌的筛选及其降解效果研究[J]. 浙江农业学报, 2018, 30(10): 1699-1704.