青海省部分地区马铃薯Y病毒cp基因序列分析

doi:10.3969/j.issn.1004-1524.2019.11.15

浙江农业学报 ›› 2019, Vol. 31 ›› Issue (11): 1888-1895.DOI: 10.3969/j.issn.1004-1524.2019.11.15

青海省部分地区马铃薯Y病毒cp基因序列分析

程亮^1,2

1.青海省农林科学院植物保护研究所,青海省农业有害生物综合治理重点实验室,青海西宁 810016;
2.青海大学,省部共建三江源生态与高原农牧业国家重点实验室,青海西宁 810016

收稿日期:2019-04-08 出版日期:2019-11-25 发布日期:2019-12-04
作者简介:程亮(1978—),男,河南林州人,硕士,研究方向为植物病理学。E-mail: liangcheng1979@163.com
基金资助:
青海省自然科学基金( 2018-ZJ-917); 青海大学中青年科研基金 (2014-QNY-7)

Analysis on cp gene sequences of potato virus Y from some places in Qinghai Province

CHENG Liang^1,2

1. Key Laboratory of Agricultural Integrated Pest Management in Qinghai Province, Institute of Plant Protection, Qinghai Provincial Academy of Agriculture and Forestry Sciences, Xining 810016, China;
2. State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining 810016, China

Received:2019-04-08 Online:2019-11-25 Published:2019-12-04

摘要/Abstract

摘要： 马铃薯Y病毒(PVY)是危害我国马铃薯的主要病毒之一,其在自然条件下极易发生基因变异而产生株系分化现象。为揭示青海马铃薯Y病毒分离物cp基因的分子变异,利用RT-PCR方法扩增获得了16个马铃薯Y病毒分离物的cp基因序列,采用SDT、DANMAN、RDP和MEGA软件对其进行分子变异分析。序列一致率结果表明,16个分离物的核苷酸序列均为801个碱基,一致率为98.0%~100%,编码267个氨基酸,一致率为94.8%~100%。重组分析表明,16个分离物未发生基因重组。系统发育分析发现,11个分离物与PVY^N-Wi株系相聚成簇,表明其在系统发育关系上与PVY^N-Wi株系的亲缘关系最近,占被检出病毒分离物的68.7%;另外5个分离物与PVY^O株系相聚成簇,表明其在系统发育关系上与PVY^O株系亲缘关系最近;未发现PVY^C株系、PVY^N株系、PVY^E株系和PVY^NTN株系存在。

关键词: 马铃薯Y病毒, cp基因, 序列分析, 最大似然法, 青海

Abstract: Potato virus Y(PVY)is one of the main viruses, which can cause serious losses in potato production. PVY is prone to gene mutation and plant differentiation under natural conditions. To understand the sequence variation of cp gene in PVY, cp gene of 16 isolates from Qinghai was amplified by RT-PCR. SDT, DNAMAN, RDP, and MEGA software were used for analysis. In multiple sequence alignments of cp gene, 16 isolates contained 801 base pairs with the identity of 98.0%-100% and encoded 267 amino acides with the identity of 94.8%-100%, respectively. Recombinant analysis indicated that no gene recombination was found in 16 isolates. Phylogenetic analysis showed that 11 isolates was clustered with isolates belonging to PVY^N-Wi strains, suggesting that the 11 PVY isolates shared high homology with PVY^N-Wi strain and accounting for 68.7% of total isolates. 5 PVY isolates were in the same branch with the reference PVY^O strains, indicating 5 PVY isolates were closer to PVY^O. PVY^C, PVY^N, PVY^E and PVY^NTN strains were not detected.

Key words: potato virus Y, cp gene, sequences analysis, maximum likelihood method, Qinghai

中图分类号:

S432.1

程亮. 青海省部分地区马铃薯Y病毒cp基因序列分析[J]. 浙江农业学报, 2019, 31(11): 1888-1895.

CHENG Liang. Analysis on cp gene sequences of potato virus Y from some places in Qinghai Province[J]. , 2019, 31(11): 1888-1895.

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青海省部分地区马铃薯Y病毒cp基因序列分析

Analysis on cp gene sequences of potato virus Y from some places in Qinghai Province

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