浙江农业学报 ›› 2019, Vol. 31 ›› Issue (9): 1493-1501.DOI: 10.3969/j.issn.1004-1524.2019.09.13

• 园艺科学 • 上一篇    下一篇

光学干涉膜下生菜的生理指标变化及品质分析

翟克清1, 张昕昱2, 周念念1, 安玉兰1, 雷玥1, 高洁1, 甘德芳1,*, 刘文2, 何晓梅3   

  1. 1.安徽农业大学 园艺学院,安徽 合肥 230036;
    2.中国科学技术大学 物理学院,安徽 合肥 230026;
    3.皖西学院,安徽 六安 237012
  • 收稿日期:2019-03-03 出版日期:2019-09-25 发布日期:2019-10-11
  • 通讯作者: *甘德芳,E-mail:gandf@ahau.edu.cn
  • 作者简介:翟克清(1993—),男,山东滨州人,硕士研究生,主要从事蔬菜分子生物学研究。E-mail: 275974456@qq.com
  • 基金资助:
    安徽省高等学校自然科学研究重点项目(KJ2019A0188,KJ2017A407); 研究生创新基金(2019ysj-9); 大学生创新项目(201710364075,XJDC2018387,201810364177); 中央高校基本科研业务费专项资金(WK6030000084)

Changes of physiological indexes and quality analysis of lettuce under optical interference film

ZHAI Keqing1, ZHANG Xinyu2, ZHOU Niannian1, AN Yulan1, LEI Yue1, GAO Jie1, GAN Defang1,*, LIU Wen2, HE Xiaomei3   

  1. 1. College of Horticulture, Anhui Agricultural University, Hefei 230036, China;
    2. School of Physical Sciences, University of Science and Technology of China, Hefei 230026, China;
    3. West Anhui University, Lu’an 237012, China
  • Received:2019-03-03 Online:2019-09-25 Published:2019-10-11

摘要: 生菜因其生长期短、产量高、易于生食等特点,已经成为设施栽培的一种重要叶菜。传统的光伏发电占用大量的土地资源,而光伏农业在满足农作物生长的光照需求下,进行光伏发电。为此,本研究以蒙黛和克里斯汀两个生菜品种作为供试品种,研究不同光学干涉薄膜(红蓝光透过膜及远红光截止膜)对生菜的抗氧化酶、营养品质及叶片超微结构等的影响。结果表明,蒙黛在红蓝光透过膜及远红光截止膜下的CAT活性、SOD活性及丙二醛含量都呈现下降的趋势;克里斯汀在两种膜覆盖下的CAT活性上升,而SOD活性及丙二醛的含量则呈现下降趋势,但两种生菜在两种膜覆盖下,后期的酶活性大多较对照的高。红蓝光透过膜下,两种生菜可溶性糖的含量先升高后降低,远红光截止环境下则呈现上升的趋势。随着覆盖时间的延长,两个品种在两种光环境下,维生素C的累积量都明显增加;蛋白质及叶绿素含量随着覆盖时间的延长,较前期有所下降,但大多比对照的高。叶片超微结构分析表明,红蓝光透过膜下,蒙黛的叶绿体聚集增大,而远红光截止环境下叶片的部分细胞破坏,叶绿体基粒分层不明显。研究结果可为光学干涉膜的技术改进、光学干涉膜的大田推广应用以及光伏农业的未来发展走向提供参考和依据。

关键词: 光学干涉膜, 生菜, 生理指标, 营养品质, 细胞超微结构

Abstract: Due to short growing period, high yield and suitable for raw food, lettuce becomes an important leafy vegetable in facility cultivation. Traditional photovoltaic power generation occupies a large amount of land resources, while photovoltaic agriculture performs photovoltaic power generation under the illumination demand that meets the growth of crops. In this study, two kinds of lettuce varieties, MonDai and Kristin were used as the tested varieties to study the bioactive enzymes, nutritional quality and the ultrastructure of the leaves under different optical interference films (red blue light transmission film and far red light-cutting film). The results showed that the CAT activity, SOD activity and malondialdehyde content of MonDai showed a downward trend under the red blue light transmission film and the far-red light-cutting film. Meanwhile, the increased activity of CAT and the reduced activity of SOD and the malondialdehyde appeared in Kristin covered with two films. However, the enzyme activities of two lettuces under two kinds of film mulching were mostly higher than those of the control. As for the nutritional quality of lettuce, the content of soluble sugar in the two lettuces increased firstly and then decreased in the red-blue light transmission film treatment, while there is an upward trend in the far red light-cutting film treatment. With the extension of the coverage time, the accumulation of vitamin C increased significantly in both light treatments and the content of protein and chlorophyll decreased, while most of them are higher than those of the control. The ultrastructural analysis of the leaves showed that the chloroplast aggregation of the MonDai increased under the red and blue light, while some cells of the leaves were destroyed under the far red light-cutting film, and the chloroplast granules were not obvious. This study provided a reference and basis for the technical improvement of optical interferometric film, the field application and the future development trend of photovoltaic agriculture.

Key words: optical interference film, lettuce, physiological index, nutritional quality, cell ultrastructure

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