外源褪黑素对NaCl胁迫下5BB葡萄叶片生理特性的影响

doi:10.3969/j.issn.1004-1524.2019.04.07

浙江农业学报 ›› 2019, Vol. 31 ›› Issue (4): 556-564.DOI: 10.3969/j.issn.1004-1524.2019.04.07

外源褪黑素对NaCl胁迫下5BB葡萄叶片生理特性的影响

韩国民¹, 刘茜¹, 唐美玲², 代玲敏^1,*

1.滨州医学院药学院,山东烟台 264003;
2.烟台市农业科学研究院葡萄与葡萄酒研究所,山东烟台 265500

收稿日期:2018-09-03 出版日期:2019-04-25 发布日期:2019-04-19
通讯作者: *代玲敏,E-mail: dailingmin@nwsuaf.edu.cn
作者简介:韩国民(1986-),男,山东泰安人,博士,讲师,主要从事葡萄与葡萄酒研究。E-mail: hanguomin@nwsuaf.edu.cn
基金资助:
现代农业产业技术体系专项资金(CARS-29-16);烟台市葡萄与葡萄酒局专项资金(50012305073)

Effects of exogenous melatonin on physiological characteristics of 5BB grape leaves under NaCl stress

HAN Guomin¹, LIU Xi¹, TANG Meiling², DAI Lingmin^1,*

1. School of Pharmacy, Binzhou Medical University, Yantai 264003, China;
2. Institute of Grape and Wine, Yantai Academy of Agricultural Sciences, Yantai 265500, China

Received:2018-09-03 Online:2019-04-25 Published:2019-04-19

摘要/Abstract

摘要： 以砧木葡萄5BB (Vitis berlandieri×V. riparia 5BB)为材料,用离体叶片培养法进行NaCl胁迫。用3种不同浓度的褪黑素(melatonin,MT)溶液(100、200、500 nmol·L^-1)分别与0.25 mol·L^-1 NaCl溶液混合并将叶片叶柄浸入其中进行培养,研究外源MT对NaCl胁迫下葡萄叶片光合作用和相关生理指标的影响。结果表明,NaCl胁迫下外源MT可有效增强超氧化物歧化酶(SOD)活性,及时清除逆境胁迫下叶片积累的氧自由基,减缓膜质过氧化过程,有效提高逆境胁迫下葡萄叶片中光合色素含量和光合能力。随着外源MT浓度的增加,对葡萄叶片的保护效果逐渐减小。外源MT能提高砧木葡萄5BB对NaCl胁迫的抵抗力,且浓度为100 nmol·L^-1时效果较好。

关键词: 葡萄, 叶片, 外源褪黑素, NaCl胁迫, 生理指标

Abstract: The detached leaves of grape rootstocks 5BB were used to study the effects of exogenous melatonin (100, 200 and 500 nmol·L^-1) on photosynthesis and related physiological indicators under NaCl (0.25 mol·L^-1) stress, with the petiole immersed in the mixed solution of melatonin and NaCl during the six days' treatment. The results showed that exogenous melatonin increased superoxide dismutase (SOD) activity to scavenge free oxygen radical accumulated in leaves under NaCl stress, and blocked membrane lipid peroxidation to improve the photosynthetic pigment content and photosynthetic capacity of grape leaves under stress; Furthermore, the protective effects of exogenous melatonin decreased with the increase of melatonin concentration. Thus, exogenous melatonin could alleviate the damage by NaCl stress in 5BB,and 100 nmol·L^-1 was the proper concentration.

Key words: grape, leaf, exogenous melatonin, NaCl stress, physiological index

中图分类号:

S663.9

韩国民, 刘茜, 唐美玲, 代玲敏. 外源褪黑素对NaCl胁迫下5BB葡萄叶片生理特性的影响[J]. 浙江农业学报, 2019, 31(4): 556-564.

HAN Guomin, LIU Xi, TANG Meiling, DAI Lingmin. Effects of exogenous melatonin on physiological characteristics of 5BB grape leaves under NaCl stress[J]. , 2019, 31(4): 556-564.

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外源褪黑素对NaCl胁迫下5BB葡萄叶片生理特性的影响

Effects of exogenous melatonin on physiological characteristics of 5BB grape leaves under NaCl stress

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