不同氮肥管理方式对稻田土壤微生物群落结构的影响

doi:10.3969/j.issn.1004-1524.2020.02.15

浙江农业学报 ›› 2020, Vol. 32 ›› Issue (2): 308-316.DOI: 10.3969/j.issn.1004-1524.2020.02.15

不同氮肥管理方式对稻田土壤微生物群落结构的影响

李美霖^1,2, 陈宇眺³, 洪晓富², 乔宇颖^2,4, 王青霞², 陈喜靖², 沈阿林², 喻曼^2,*

1.浙江农林大学环境与资源学院,浙江杭州 311300;
2.浙江省农业科学院环境资源与土壤肥料研究所,浙江杭州 310021;
3.浙江省农业科学院作物与核技术利用研究所,浙江杭州 310021;
4.西北农林科技大学资源环境学院,陕西杨凌 712100

收稿日期:2019-08-30 出版日期:2020-02-25 发布日期:2020-03-13
通讯作者: *喻曼,E-mail:yuman20030703@aliyun.com
作者简介:李美霖(1991—),女,山东临沂人,硕士研究生,主要从事土壤微生物研究。E-mail:2512479350@qq.com
基金资助:
国家重点研发计划(2016YFD0200804,2018YFD0200507); 浙江省自然科学基金(LQ18D030001); 浙江省重点研发计划(2015C03013)

Effects of nitrogen management on soil microbial community structure in paddy fields

LI Meilin^1,2, CHEN Yutiao³, HONG Xiaofu², QIAO Yuying^2,4, WANG Qingxia², CHEN Xijing², SHEN Alin², YU Man^2,*

1. College of Environment and Resources, Zhejiang A&F University, Hangzhou 311300,China;
2. Institute of Environment, Resource, Soil and Fertilizer, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
3. Institute of Crops and Utilization of Nuclear Technology, Zhejiang Academy of Agricutural Sciences, Hangzhou 310021, China;
4. College of Resources and Environment, Northwest A&F University, Yangling 712100, China

Received:2019-08-30 Online:2020-02-25 Published:2020-03-13

摘要/Abstract

摘要： 为合理使用氮肥,维持稻田生态健康发展,运用磷脂脂肪酸(PLFA)和Biolog-Eco微平板法研究了4种氮肥管理模式:不施氮肥(CK)、农民习惯施肥(FP)、氮肥优化管理1(OPT1)、氮肥优化管理2(OPT2)对稻田土壤微生物群落结构的影响。结果表明:OPT1处理较CK提高了土壤微生物的代谢活性和碳源利用能力,而FP、OPT2处理均较CK降低了土壤微生物代谢活性和碳源利用能力。OPT1处理的土壤总生物量最高,为42.11 nmol·g^-1,OPT2处理的土壤总生物量为40.16 nmol·g^-1,均高于CK的34.19 nmol·g^-1,而FP处理的土壤总生物量为34.16 nmol·g^-1,低于CK。OPT1处理的土壤微生物多样性较复杂,F/B值最高。综合各项指标,OPT1处理更有利于土壤生态环境稳定。

关键词: 稻田, 土壤微生物, 磷脂脂肪酸, 氮肥管理

Abstract: The microbial communities in four nitrogen fertilizer management modes, namely, zero-nitrogen treatment (CK), farmers’ conventional cultivation pattern (FP), modified farmers’ conventional cultivation pattern 1 (OPT1), and modified farmers’ conventional cultivation pattern 2 (OPT2), were studied by phospholipid fatty acid (PLFA) and Biolog-Eco methods to provide references for the rational utilization of nitrogen fertilizer in paddy fields. It was shown that the microbial metabolic activity and carbon source utilization ability increased in OPT1, while reduced in FP and OPT2 compared to CK. The highest amount of total biomass appeared in OPT1 with 42.11 nmol·g^-1, and was followed by OPT2 with 40.16 nmol·g^-1, which were both higher than that in CK (34.19 nmol·g^-1), but the amount of total biomass in FP was 34.16 nmol·g^-1, which was lower than that in CK. Moreover, the diversity of microbial community in OPT1 was more complex, and F/B value was the highest among the 4 treatments. In summary, the OPT1 treatment was more conducive to the stability of ecological environment in soil.

Key words: paddy, soil microbes, phospholipid fatty acid, nitrogen fertilizer management

中图分类号:

S154.3

李美霖, 陈宇眺, 洪晓富, 乔宇颖, 王青霞, 陈喜靖, 沈阿林, 喻曼. 不同氮肥管理方式对稻田土壤微生物群落结构的影响[J]. 浙江农业学报, 2020, 32(2): 308-316.

LI Meilin, CHEN Yutiao, HONG Xiaofu, QIAO Yuying, WANG Qingxia, CHEN Xijing, SHEN Alin, YU Man. Effects of nitrogen management on soil microbial community structure in paddy fields[J]. , 2020, 32(2): 308-316.

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不同氮肥管理方式对稻田土壤微生物群落结构的影响

Effects of nitrogen management on soil microbial community structure in paddy fields

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