pH对百子莲体胚诱导期生理特性的影响

doi:10.3969/j.issn.1004-1524.2020.08.10

浙江农业学报 ›› 2020, Vol. 32 ›› Issue (8): 1405-1414.DOI: 10.3969/j.issn.1004-1524.2020.08.10

pH对百子莲体胚诱导期生理特性的影响

岳建华^1,2, 董艳³, 李文杨³, 李蒙^1,2, 张琰^1,2,*

1.信阳农林学院园艺学院,河南信阳 464100;
2.信阳市大别山区园艺植物遗传改良重点实验室,河南信阳 464100;
3.信阳农林学院林学院,河南信阳 464100

收稿日期:2020-01-09 出版日期:2020-08-25 发布日期:2020-08-28
通讯作者: *,张琰,E-mail: zhy8010878@163.com
作者简介:岳建华(1982—),男,河南郑州人,博士,讲师,主要从事园艺植物发育研究。E-mail: jhyues@163.com
基金资助:
河南省科技攻关计划(172102110248); 河南省基础与前沿研究计划(162300410181); 信阳农林学院青年教师科研基金(201701014); 信阳农林学院园艺专业综合改革试点建设项目(ZYZHGG201803)

Effects of pH on physiological characters in somatic embryo induction stage of Agapanthus praecox

YUE Jianhua^1,2, DONG Yan³, LI Wenyang³, LI Meng^1,2, ZHANG Yan^1,2,*

1. College of Horticulture, Xinyang Agriculture and Forestry University, Xinyang 464100, China;
2. Key Laboratory of Horticultural Plants Genetic Improvement of Dabie Mountain, Xinyang 464100, China;
3. College of Forestry, Xinyang Agriculture and Forestry University, Xinyang 464100, China

Received:2020-01-09 Online:2020-08-25 Published:2020-08-28

摘要/Abstract

摘要： 合适的培养基pH可维持离体培养所需的酸碱环境,保证细胞的正常生理代谢。为了优化百子莲体胚诱导期培养基pH,并初步揭示不同pH对百子莲内源激素、碳源代谢、氧化胁迫等生理特性的影响,以百子莲小花梗为外植体诱导的胚性愈伤组织为试材,研究梯度pH(5.6、5.8、6.0、6.2)对幼胚(YSE)、成熟胚(MSE)诱导的影响,用酶联免疫法(ELISA)测定不同pH处理下YSE、MSE生理特性的差异,并将pH、体胚数量、生理指标进行相关性分析。结果表明,YSE、MSE阶段较佳的培养基pH分别为6.0和6.2。YSE诱导阶段,内源IAA含量与YSE数量呈极显著(P<0.01)正相关,而GA₁含量与YSE数量呈显著(P<0.05)负相关。MSE阶段,pH与MSE数量、IAA氧化酶活性、蔗糖含量呈显著(P<0.05)正相关,IAA氧化酶活性与MSE数量呈极显著(P<0.01)正相关,GA₄含量、蔗糖含量与MSE数量均具有较强的相关性。H₂O₂与YSE数量呈负相关,与MSE数量呈正相关,POD活性与YSE数量呈显著(P<0.05)负相关。本研究优化了体胚诱导期的pH参数,研究结果表明,内源IAA可能是不同pH环境下调控YSE、MSE诱导数量的关键因子,GA₄、蔗糖含量在MSE诱导中发挥重要作用。

关键词: 百子莲, pH, 体细胞胚胎发生, 植物激素, 糖代谢, 氧化胁迫

Abstract: Suitable medium pH maintains a suitable pH environment in the process of in vitro culture, which ensures the normal physiological metabolism of cells. To improve somatic embryogenesis efficiency in Agapanthus praecox, the medium pH parameter was optimized, and the regulatory effects of pH on plant hormones, carbon sources and oxidative stress during somatic embryogenesis were investigated. Pedicel originated embryogenic callus were used as materials, the induction efficiency of young somatic embryos (YSE) and mature somatic embryos (MSE) with gradient medium pH (5.6, 5.8, 6.0 and 6.2) in somatic embryo induction stage were revealed, respectively. Furthermore, physiological differences were detected by the ELISA technique, and correlation analyses among pH, embryo number and physiological indices were conducted. The results showed that the optimal pH values in YSE and MSE induction stages were 6.0 and 6.2, respectively. In YSE induction stage, content of endogenous IAA was positively correlated with YSE number (P<0.01), while content of GA₁ was negatively correlated with YSE number (P<0.05). In MSE induction stage, pH was positively correlated with MSE quantity, IAA oxidase activity, and sucrose content (P<0.05), respectively. Then IAA oxidase activity was positively correlated with MSE quantity (P<0.01), and the correlation between GA₄ content, sucrose content and MSE quantity were also strong. H₂O₂ content and YSE numbers showed a positive correlation, while the correlation between H₂O₂ and MSE numbers was negative. POD activity was negatively correlated with YSE numbers (P<0.05). pH in the process of YSE and MSE induction stages were optimized in this study, the results showed that endogenous IAA might be the crucial factor for somatic embryo induction under different pH conditions, GA₄ and sucrose contents played important roles in MSE induction.

Key words: Agapanthus praecox, pH, somatic embryogenesis, plant hormone, sugar metabolism, oxidative stress

中图分类号:

S682.1⁺9

岳建华, 董艳, 李文杨, 李蒙, 张琰. pH对百子莲体胚诱导期生理特性的影响[J]. 浙江农业学报, 2020, 32(8): 1405-1414.

YUE Jianhua, DONG Yan, LI Wenyang, LI Meng, ZHANG Yan. Effects of pH on physiological characters in somatic embryo induction stage of Agapanthus praecox[J]. , 2020, 32(8): 1405-1414.

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pH对百子莲体胚诱导期生理特性的影响

Effects of pH on physiological characters in somatic embryo induction stage of Agapanthus praecox

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