中链脂肪酸及其酯对致病菌的抑菌作用

doi:10.3969/j.issn.1004-1524.2021.09.05

浙江农业学报 ›› 2021, Vol. 33 ›› Issue (9): 1611-1616.DOI: 10.3969/j.issn.1004-1524.2021.09.05

中链脂肪酸及其酯对致病菌的抑菌作用

汤佳宁¹(), 王永侠¹, 刘金松², 曾新福², 杨彩梅²^,^*()

1.浙江农林大学动物科技学院,浙江杭州 311300
2.浙江惠嘉生物科技股份有限公司,浙江安吉 311107

收稿日期:2020-08-14 出版日期:2021-09-25 发布日期:2021-10-09
通讯作者: 杨彩梅
作者简介:* 杨彩梅,E-mail: yangcaimei2012@163.com
汤佳宁(1995—),女,陕西汉中人,硕士研究生,研究方向为动物营养。E-mail: 2018820662011@stu.zafu.edu.cn
基金资助:
浙江省重点研发计划(2019C02051);国家重点研发计划政府间国际创新合作项目(2018YFE0112700);浙江省自然科学基金(LY17C170004)

Bacteriostatic effect of medium-chain fatty acids and their esters on pathogenic bacteria

TANG Jianing¹(), WANG Yongxia¹, LIU Jinsong², ZENG Xinfu², YANG Caimei²^,^*()

1. College of Animal Science And Technology, Zhejiang A & F University, Hangzhou 311300, China
2. Zhejiang Huijia Bio-Technology Co., Ltd., Anji 311107, China

Received:2020-08-14 Online:2021-09-25 Published:2021-10-09
Contact: YANG Caimei

摘要/Abstract

摘要：

中链脂肪酸甘油酯已经成为抗生素替代物研究的新热点。文章旨在探究月桂酸、单辛酸甘油酯、单月桂酸甘油酯、辛葵酸甘油酯对大肠埃希菌、金黄色葡萄球菌、肠炎沙门氏菌和鸡白痢沙门氏菌的抑菌效果。结果显示:月桂酸、单月桂酸甘油酯、单辛酸甘油酯对大肠埃希菌有显著(P<0.05)抑制作用;月桂酸、单月桂酸甘油酯、单辛酸甘油酯、辛葵酸甘油酯对金黄色葡萄球菌有显著(P<0.05)抑制作用;月桂酸、单月桂酸甘油酯、单辛酸甘油酯、辛葵酸甘油酯对肠炎沙门氏菌有显著(P<0.05)抑制作用;月桂酸、单月桂酸甘油酯、单辛酸甘油酯对鸡白痢沙门氏菌有显著(P<0.05)抑制作用。其中,月桂酸、单月桂酸甘油酯、单辛酸甘油酯对4种致病菌的作用效果随时间延长而增强。

关键词: 中链脂肪酸脂, 大肠埃希菌, 肠炎沙门氏菌, 金黄色葡萄球菌, 鸡白痢沙门氏菌, 抑菌效果

Abstract:

Medium-chain fatty acid glycerides have become a new hot spot in the research of antibiotic substitutes. The purpose of this study was to investigate the bacteriostatic effects of lauric acid, glycerol monooctanoate, glycerol monolaurate and glycerol octanoate on Escherichia coli, Staphylococcus aureus, Salmonella enteritidis and Salmonella pullorum. The results showed that four medium-chain fatty acid glycerides had certain inhibitory effects on four kinds of pathogenic bacteria. Among them, the effects of lauric acid, glycerol monolaurate and glycerol monooctanoate on four kinds of pathogenic bacteria increased with time.

Key words: medium chain fatty acids, Escherichia coli, Salmonella enteritidis, Staphylococcus aureus, Salmonella pullorum, bacteriostatic effect

中图分类号:

S859

汤佳宁, 王永侠, 刘金松, 曾新福, 杨彩梅. 中链脂肪酸及其酯对致病菌的抑菌作用[J]. 浙江农业学报, 2021, 33(9): 1611-1616.

TANG Jianing, WANG Yongxia, LIU Jinsong, ZENG Xinfu, YANG Caimei. Bacteriostatic effect of medium-chain fatty acids and their esters on pathogenic bacteria[J]. Acta Agriculturae Zhejiangensis, 2021, 33(9): 1611-1616.

图/表 4

图1 不同中链脂肪酸甘油酯对大肠埃希菌的抑菌作用同一时间不同处理间没有相同字母表示差异显著(P<0.05)。下同。

Fig.1 Antibacterial effects of different medium-chain fatty acid glycerides on Escherichia coli The values with different letters in the same time showed significant difference (P<0.05). The same as below.

图2 不同中链脂肪酸甘油酯对金黄色葡萄球菌的抑菌作用

Fig.2 Antibacterial effects of different medium-chain fatty acid glycerides on Staphylococcus aureus

图3 不同中链脂肪酸甘油酯对肠炎沙门氏菌的抑菌作用

Fig.3 Antibacterial effect of different medium-chain fatty acid glycerides on Salmonella enteritidis

图4 不同中链脂肪酸甘油酯对鸡白痢沙门氏菌的抑菌作用

Fig.4 Antibacterial effect of different medium-chain fatty acid glycerides on Salmonella pullorum

参考文献 20

[1]	LOUNG F S, SILALAHI J, SURYANTO D. Antibacterial activity of enzymatic hydrolyzed of virgin coconut oil and palm kernel oil against Staphylococcus aureus, Salmonella thypi and Escherichia coli[J]. International Journal of Pharmtech Research, 2014, 6(2):628-633.
[2]	张立伟, 石玉祥, 张永英, 等. 鸡源大肠杆菌毒力基因检测及分子流行特征[J]. 微生物学报, 2020, 60(11):2498-2510.
	ZHANG L W, SHI Y X, ZHANG Y Y, et al. Analysis of virulence genes and molecular characteristics of chicken-derived Escherichia coli[J]. Acta Microbiologica Sinica, 2020, 60(11):2498-2510.(in Chinese with English abstract)
[3]	STEFANI S, CHUNG D R, LINDSAY J A, et al. Meticillin-resistant Staphylococcus aureus(MRSA): global epidemiology and harmonisation of typing methods[J]. International Journal of Antimicrobial Agents, 2012, 39(4):273-282. DOI URL
[4]	XIE S, LI Y C, ZHAO S Y, et al. Salmonella infection induced intestinal crypt hyperplasia through Wnt/β-catenin pathway in chicken[J]. Research in Veterinary Science, 2020, 130:179-183. DOI URL
[5]	孙晓鸣. 鸡白痢诊断与治疗[J]. 畜牧兽医科学(电子版), 2020(2):114-115.
	SUN X M. Diagnosis and treatment of chicken pullorum[J]. Graziery Veterinary Sciences (Electronic Version), 2020(2):114-115.(in Chinese)
[6]	何凤琴, 刘宏山. 中链脂肪酸甘油酯在动物生产中的研究与应用[J]. 畜牧与兽医, 2018, 50(7):137-140.
	HE F Q, LIU H S. Research on application of medium-chain triglyceride in animal production[J]. Animal Husbandry & Veterinary Medicine, 2018, 50(7):137-140.(in Chinese with English abstract)
[7]	LEE H F, CHIANG S H. Energy value of medium-chain triglycerides and their efficacy in improving survival of neonatal pigs1[J]. Journal of Animal Science, 1994, 72(1):133-138. DOI URL
[8]	LI Q S, ESTES J D, SCHLIEVERT P M, et al. Glycerol monolaurate prevents mucosal SIV transmission[J]. Nature, 2009, 458(7241):1034-1038. DOI URL
[9]	杨博, 马永喜, 沈水宝. 脂类在饲料行业中应用的新思考[J]. 动物营养学报, 2019, 31(11):4901-4908.
	YANG B, MA Y X, SHEN S B. Rethinking of using lipids in feed industry[J]. Chinese Journal of Animal Nutrition, 2019, 31(11):4901-4908.(in Chinese with English abstract)
[10]	刘梦芸, 陈晓倩, 赵豪斌, 等. 饲粮中添加单月桂酸甘油酯对肉鸡生长性能、消化能力及鸡肉营养品质的影响[J]. 食品科学, 2018, 39(15):67-71.
	LIU M Y, CHEN X Q, ZHAO H B, et al. Effect of dietary supplementation with glycerol monolaurate on growth performance, digestive ability and chicken nutritional components of broilers[J]. Food Science, 2018, 39(15):67-71.(in Chinese with English abstract)
[11]	刘梦芸, 王建莉, 冯凤琴. 饲粮添加单月桂酸甘油酯对蛋鸡生产性能、蛋品质、血清生化指标、免疫器官指数和腹脂形态的影响[J]. 中国家禽, 2017, 39(17):24-30.
	LIU M Y, WANG J L, FENG F Q. Effects of glycerol monolaurate on production performance, egg quality, serum parameters, immune organ index and abdominal fat morphology of laying hens[J]. China Poultry, 2017, 39(17):24-30.(in Chinese with English abstract)
[12]	薛永强, 黄志威, 雷志伟, 等. 中短链脂肪酸在无抗饲料中的应用[J]. 饲料研究, 2020(3):133-136.
	XUE Y Q, HUANG Z W, LEI Z W, et al. Application of short-medium chain fatty acids in non-resistant feed[J]. Feed Research, 2020(3):133-136.(in Chinese with English abstract)
[13]	刁慧, 张锦秀, 张纯, 等. 中链脂肪酸在仔猪营养中的生理功能研究进展[J]. 中国畜牧杂志, 2020, 56(2):33-38.
	DIAO H, ZHANG J X, ZHANG C, et al. Advances in physiological function of medium-chain fatty acids in piglet nutrition[J]. Chinese Journal of Animal Science, 2020, 56(2):33-38.(in Chinese with English abstract)
[14]	KITAHARA T, AOYAMA Y, HIRAKATA Y, et al. In vitro activity of lauric acid or myristylamine in combination with six antimicrobial agents against methicillin-resistant Staphylococcus aureus(MRSA)[J]. International Journal of Antimicrobial Agents, 2006, 27(1):51-57. DOI URL
[15]	YEN H C, LAI W K, LIN C S, et al. Medium-chain triglyceride as an alternative of in-feed colistin sulfate to improve growth performance and intestinal microbial environment in newly weaned pigs[J]. Animal Science Journal, 2015, 86(1):99-104. DOI URL
[16]	赵存洋. 单月桂酸甘油酯的抑菌性能及机理研究[D]. 南昌: 南昌大学, 2013.
	ZHAO C Y. Study on antibacterial properties of monolaurin and its mechanism[D]. Nanchang: Nanchang University, 2013.(in Chinese with English abstract)
[17]	LIEBERMAN S, ENIG M G, PREUSS H G. A review of monolaurin and lauric acid: natural virucidal and bactericidal agents[J]. Alternative and Complementary Therapies, 2006, 12(6):310-314. DOI URL
[18]	FORTUOSO B F, DOS REIS J H, GEBERT R R, et al. Glycerol monolaurate in the diet of broiler chickens replacing conventional antimicrobials: Impact on health, performance and meat quality[J]. Microbial Pathogenesis, 2019, 129:161-167. DOI URL
[19]	KIMSEY H R, ADAMS D M, KABARA J J. Increased inactivation of bacterial spores at high temperatures in the presence of monoglycerides2[J]. Journal of Food Safety, 1981, 3(2):69-82. DOI URL
[20]	张佩华, 梅子, 傅玉颖, 等. 食品级单辛酸甘油酯微乳液的抑菌研究[J]. 食品工业科技, 2013, 34(13):136-138.
	ZHANG P H, MEI Z, FU Y Y, et al. Antimicrobial activity evaluation of food grade glycerol mono-octanoate microemulsion[J]. Science and Technology of Food Industry, 2013, 34(13):136-138.(in Chinese with English abstract)

中链脂肪酸及其酯对致病菌的抑菌作用

Bacteriostatic effect of medium-chain fatty acids and their esters on pathogenic bacteria

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 4

参考文献 20

相关文章 12

编辑推荐

Metrics

本文评价

[1]	江宇航, 辛维岗, 张棋麟, 邓先余, 王峰, 林连兵. 霉变饲用玉米真菌的分离、鉴定与乳酸菌素对其的防霉抑菌效果[J]. 浙江农业学报, 2021, 33(7): 1283-1291.
[2]	孟司奇, 丁高蓝, 肖兴宁, 肖英平, 杨华, 汪雯. 巴氏杀菌乳中金黄色葡萄球菌的生长预测模型构建[J]. 浙江农业学报, 2020, 32(7): 1281-1288.
[3]	杨华, 马艳, 刘秀婷, 吕文涛, 卢立志, 肖英平. 即食鸭制品大肠埃希菌污染状况、毒力基因和耐药特征分析[J]. 浙江农业学报, 2020, 32(10): 1841-1848.
[4]	常江, 罗怡, 唐标, 张玲, 戴贤君, 裘罕琦, 杨华, 夏效东. 一株分离自鸡肉样品的多重耐药大肠埃希菌的全基因组测序及耐药性研究[J]. 浙江农业学报, 2019, 31(8): 1249-1256.
[5]	彭珂楠, 周雪珂, 殷鑫欢, 李飞, 蔡瑶, 曾喻兵, 江朝源, 张儒博, 杨泽晓, 徐志文, 朱玲. 四川地区猪源致病性大肠埃希菌分子分群、生物被膜形成能力及耐药性研究[J]. 浙江农业学报, 2019, 31(10): 1599-1607.
[6]	王宇, 李碧, 阳明贤, 左之才. 奶牛隐性乳房炎三种致病菌的多重PCR检测方法的建立[J]. 浙江农业学报, 2019, 31(1): 20-29.
[7]	刘翠艳, 陈四玉, 孙桃桃, 尚莉莉, 杨明川, 杨孜生, 韩春杨. 马齿苋-益生菌联用对热毒证小鼠腹泻的预防效果[J]. 浙江农业学报, 2018, 30(2): 211-219.
[8]	杨华, 何祥祥, 肖英平, 钱鸣蓉, 张巧艳, 唐标. 浙江省三地区冷鲜鸡中大肠埃希菌耐药谱测定及MLST分子分型分析[J]. 浙江农业学报, 2017, 29(6): 888-893.
[9]	黄书伦, 郑凯文, 胡洋, 李章程, 程方俊, 宋振辉, 周作勇, 刘国林, 林春发, 林永润, 张婷婷. 鸭源大肠杆菌Ⅰ型菌毛的鉴定及FimA、FimH基因特征分析[J]. 浙江农业学报, 2017, 29(12): 1994-1999.
[10]	刘勇亮, 陈波, 刘明刚, 徐毓琴, 戴茜茜, 谷笑笑, 潘康成. 连梅止痢中药制剂对大肠埃希菌K88感染仔猪的疗效[J]. 浙江农业学报, 2016, 28(9): 1493-1500.
[11]	王震，尹红梅，刘标，杜东霞，许隽，贺月林*. 发酵床垫料中优势细菌的分离鉴定及生物学特性研究[J]. 浙江农业学报, 2015, 27(1): 87-.
[12]	奉代力;王强;*;郑纪慈;张昌朋;仇厚援. 几种杀菌剂对番茄灰霉病菌的抑菌效果对比研究[J]. , 2013, 25(1): 0-123.