柯乐猪PRLR基因多态性与繁殖性状的关联性

doi:10.3969/j.issn.1004-1524.2023.03.08

浙江农业学报 ›› 2023, Vol. 35 ›› Issue (3): 556-564.DOI: 10.3969/j.issn.1004-1524.2023.03.08

柯乐猪PRLR基因多态性与繁殖性状的关联性

杨酸¹(), 郭小江², 杨红文², 熊力², 李晨², 谭元成¹, 王春源¹, 张依裕¹^,^*()

1.贵州大学动物科学学院,高原山地动物遗传育种与繁殖教育部重点实验室,贵州贵阳 550025
2.贵州省农业农村厅,贵州贵阳 550001

收稿日期:2022-05-07 出版日期:2023-03-25 发布日期:2023-04-07
作者简介:杨酸(1998—),女,贵州龙里人,硕士研究生,研究方向为动物遗传育种与繁殖。E-mail: 1535390590@qq.com
通讯作者: ^*张依裕,E-mail: zyy8yyc@163.com
基金资助:
贵州省科技支撑计划(黔科合支撑〔2021〕一般147号);贵州省优秀青年科技人才培养计划(黔科合平台人才〔2021〕5630号);贵州省生猪产业发展项目(2021)

Correlation of PRLR gene polymorphisms and reproductive traits in Kele pig

YANG Suan¹(), GUO Xiaojiang², YANG Hongwen², XIONG Li², LI Chen², TAN Yuancheng¹, WANG Chunyuan¹, ZHANG Yiyu¹^,^*()

1. Key Laboratory of Plateau Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Animal Science, Guizhou University, Guiyang 550025, China
2. Department of Agriculture and Rural Affairs of Guizhou Province, Guiyang 550001, China

Received:2022-05-07 Online:2023-03-25 Published:2023-04-07

摘要/Abstract

摘要：

本研究旨在探究催乳素受体(prolactin receptor,PRLR)基因的单核苷酸突变位点(single nucleotide polymorphism,SNP)及其对柯乐猪繁殖性状的遗传效应,以期为提高柯乐猪的繁殖性能研究提供基因标记。采用PCR产物直接测序法对204头柯乐猪PRLR基因外显子区的SNP位点进行筛查鉴定,同时利用SPSS软件中单因子方差法分析各SNPs不同基因型和双倍型与7个繁殖性状的相关性,并通过在线软件分析碱基变异对mRNA二级结构的影响。结果显示,在柯乐猪PRLR基因第8外显子检测到g.20655220 C>T突变,其导致丝氨酸变成脯氨酸,为错义突变;在第4内含子检测到2个同义突变位点g.20648859 C>T和g.20648931 C>T,3个SNPs均存在3种基因型,g.20648931 C>T属于低度多态,其余2个位点属于中度多态;经卡方χ²检验,g.20655220 C>T位点的基因型分布符合Hardy-Weinberg平衡(P>0.05),g.20648859 C>T和g.20648931 C>T 位点的基因型分布极显著(P<0.01)偏离Hardy-Weinberg平衡;g.20655220 C>T位点导致PRLR基因mRNA序列二级结构发生改变,致使mRNA二级结构稳定性降低;3个SNPs共组成5种单倍型和9种双倍型,优势单倍型H1(CCC)频率为0.610,优势双倍型H1H1(CCCCCC)频率为0.382。关联性分析结果显示,g.20655220 C>T位点CC基因型母猪的初生个体重、断奶窝重和断奶个体重均显著(P<0.05)高于CT基因型,g.20648931 C>T位点CT基因型母猪的初生个体重、初生窝重和断奶窝重均显著(P<0.05)高于CC基因型和TT基因型,且该位点在初生个体重上,CC基因型显著(P<0.05)高于TT基因型;双倍型H1H5(CTCTCT)在产仔数和活仔数上为优势双倍型,H2H3(CCTTCT)在断奶窝重和断奶个体重上为较优双倍型。综上,PRLR基因可作为候选基因参与柯乐猪繁殖性状的分子标记辅助选择。

关键词: PRLR基因, 柯乐猪, 多态性, 繁殖性状

Abstract:

The aim of this study was to investigate the polymorphisms of the prolactin receptor gene (PRLR) and their effects on reproductive performance in Kele pigs, with a view to finding molecular markers for improving reproductive performance in Kele pigs. In this study, SNPs in the exon region of the PRLR gene were screened in 204 Kele pigs and identified by direct PCR product sequencing. At the same time, the correlation between different genotypes and diplotypes of SNPs and seven reproductive traits was analysed by single-factor variance method in SPSS software, and the influence of base variation on the secondary structure of mRNA was analysed by online software. The results showed that g.20655220C>T mutation was detected in the 8th exon of PRLR gene, which caused serine to proline change, which was a missense mutation. Two synonymous mutations g.20648859C>T and g.20648931C>T were detected in the fourth intron. There were three genotypes in all three SNPs, g.20648931C>T was low polymorphism and the other two loci were medium polymorphism. Chi-squared test showed that the genotype distribution of g.20655220C>T loci was in Hardy-Weinberg equilibrium (P>0.05). The genotypes of g.20648859C>T and g.20648931C>T deviated significantly(P<0.01) from Hardy-Weinberg equilibrium; g.20655220C>T locus led to changes in the secondary structure of the PRLR gene mRNA sequence, resulting in decreased stability of the secondary structure of the mRNA; the three SNPs consisted of 5 haplotypes and 9 diplotypes. The dominant haplotype H1 (CCC) frequency was 0.610 and the dominant diplotype H1H1 (CCCCCC) frequency was 0.382. Correlation analysis showed that the average birth weight, weaning litter weight and weaning average weight of CC genotype were significantly(P<0.05) higher than those of CT genotype. CT genotype of g.20648931c>T locus had significantly (P<0.05) higher birth weight, birth litter weight and weaning litter weight than those of CC genotype and TT genotype, and CC genotype had significantly (P<0.05) higher birth weight than that of TT genotype. H1H5 (CTCTCT) was the dominant doublet type in terms of litter size and live litter size, whereas H2H3 (CCTTCT) was the superior doublet type in terms of litter weight and average weaning weight. In conclusion, PRLR gene could be used as a candidate gene for molecular marker-assisted selection of breeding traits in Kele pig.

Key words: PRLR gene, KeLe pig, polymorphism, reproductive traits

中图分类号:

S828

杨酸, 郭小江, 杨红文, 熊力, 李晨, 谭元成, 王春源, 张依裕. 柯乐猪PRLR基因多态性与繁殖性状的关联性[J]. 浙江农业学报, 2023, 35(3): 556-564.

YANG Suan, GUO Xiaojiang, YANG Hongwen, XIONG Li, LI Chen, TAN Yuancheng, WANG Chunyuan, ZHANG Yiyu. Correlation of PRLR gene polymorphisms and reproductive traits in Kele pig[J]. Acta Agriculturae Zhejiangensis, 2023, 35(3): 556-564.

图/表 9

参考文献 30

[1]	杨莲, 燕志宏, 黄维江, 等. 纯种柯乐猪与巴×柯杂交猪肠道菌群结构的研究[J]. 动物营养学报, 2021, 33(3): 1359-1371. DOI
	YANG L, YAN Z H, HUANG W J, et al. Study on intestinal microflora structure of pure-bred Kele pigs and Berkshire×Kele hybrid pigs[J]. Chinese Journal of Animal Nutrition, 2021, 33(3): 1359-1371. (in Chinese with English abstract)
[2]	赵春萍, 张雄, 张静, 等. 柯乐猪的肉品质和风味指标测定及相关性分析[J]. 贵州畜牧兽医, 2022, 46(1): 12-16.
	ZHAO C P, ZHANG X, ZHANG J, et al. Determination and correlation analysis of meat quality and flavor index of Kele pig[J]. Guizhou Journal of Animal Husbandry & Veterinary Medicine, 2022, 46(1): 12-16. (in Chinese)
[3]	陈泳. 柯乐猪生长发育和胴体肉质变化规律研究[D]. 贵阳: 贵州大学, 2021.
	CHEN Y. Study on growth and development and carcass meat quality of Kele pig[D]. Guiyang: Guizhou University, 2021. (in Chinese with English abstract)
[4]	HUI Y T, YANG Y Q, LIU R Y, et al. Significant association of APOA5 and APOC3 gene polymorphisms with meat quality traits in Kele pigs[J]. Genetics and Molecular Research, 2013, 12(3): 3643-3650. DOI URL
[5]	REN J, MAO H, ZHANG Z, et al. A 6-bp deletion in the TYRP1 gene causes the brown colouration phenotype in Chinese indigenous pigs[J]. Heredity, 2011, 106(5): 862-868. DOI
[6]	黄维江, 燕志宏, 杨蓉, 等. 放牧和圈养对柯乐猪的屠宰性能及肉质性能的影响[J]. 中国畜牧杂志, 2021, 57(2): 201-205.
	HUANG W J, YAN Z H, YANG R, et al. Effects of grazing and captivity on slaughter performance and meat quality of Kele pig[J]. Chinese Journal of Animal Science, 2021, 57(2): 201-205. (in Chinese)
[7]	杨莲. 柯乐猪与柯杂猪肠道微生物差异性及其对耐粗饲特性的影响研究[D]. 贵阳: 贵州大学, 2021.
	YANG L. Study on the differences of intestinal microorganisms of kele pigs and hybrid pigs and their effects on rough feeding[D]. Guiyang: Guizhou University, 2021. (in Chinese with English abstract)
[8]	曹林, 孔凡勇, 周建雄, 等. PIC母猪窝产活仔数与初生重、断奶重、断奶成活率的相关性研究[C]// 第六届云南省科协学术年会暨红河流域发展论坛论文集:专题一: 红河流域特色产业转型升级. 红河: 云南省科学技术协会, 2016: 172-175.
[9]	汪泽华. 大约克母猪的几个主要繁殖性状表型相关分析[J]. 浙江畜牧兽医, 2001, 26(1): 4-5.
	WANG Z H. Phenotypic correlation analysis of several main reproductive traits in Yorkshire sows[J]. Zhejiang Journal of Animal Science and Veterinary Medicine, 2001, 26(1): 4-5. (in Chinese)
[10]	董林松, 谈成, 吴珍芳, 等. 母系猪繁殖性状的基因组选择策略[J]. 中国畜牧杂志, 2019, 55(8): 25-29.
	DONG L S, TAN C, WU Z F, et al. Strategy for genomic selection in reproduction traits in maternal-line pigs[J]. Chinese Journal of Animal Science, 2019, 55(8): 25-29. (in Chinese with English abstract)
[11]	BAKOEV S, GETMANTSEVA L, BAKOEV F, et al. Survey of SNPs associated with total number born and total number born alive in pig[J]. Genes, 2020, 11(5): 491. DOI URL
[12]	ZAK L J, GAUSTAD A H, BOLARIN A, et al. Genetic control of complex traits, with a focus on reproduction in pigs[J]. Molecular Reproduction and Development, 2017, 84(9): 1004-1011. DOI PMID
[13]	SCHENNINK A, TROTT J F, FREKING B A, et al. A novel first exon directs hormone-sensitive transcription of the pig prolactin receptor[J]. Journal of Molecular Endocrinology, 2013, 51(1): 1-13.
[14]	周胜花, 宋献艺. 催乳素(PRL)及其受体基因在猪中应用的研究进展[J]. 畜牧兽医科技信息, 2013(3): 9-12.
	ZHOU S H, SONG X Y. Research progress on the application of prolactin (PRL) and PRL receptor gene in pigs[J]. Chinese Journal of Animal Husbandry and Veterinary Medicine, 2013(3): 9-12. (in Chinese)
[15]	ONTERU S K, ROSS J W, ROTHSCHILD M F. The role of gene discovery, QTL analyses and gene expression in reproductive traits in the pig[J]. Society of Reproduction and Fertility Supplement, 2009, 66: 87-102. PMID
[16]	李娜. PRLR基因在5个猪种中的群体遗传特性分析[J]. 猪业科学, 2015, 32(7): 112-113.
	LI N. Analysis of population genetic characteristics of PRLR gene in five pig breeds[J]. Swine Industry Science, 2015, 32(7): 112-113. (in Chinese)
[17]	刘轩, 强巴央宗, 王强, 等. 藏猪繁殖性状多基因效应分析[J]. 遗传, 2010, 32(5): 480-485.
	LIU X, CHAMBA Y, WANG Q, et al. Effects of multi-genes for reproductive traits in Tibet pig[J]. Hereditas, 2010, 32(5): 480-485. (in Chinese with English abstract)
[18]	TERMAN A. Effect of the polymorphism of prolactin receptor (PRLR) and leptin (LEP) genes on litter size in Polish pigs[J]. Journal of Animal Breeding and Genetics, 2005, 122(6): 400-404. PMID
[19]	张陈华, 丁月云, 王阳, 等. 圩猪和定远猪PRLR基因多态性与产仔数的关联分析[J]. 中国农业大学学报, 2011, 16(3): 117-121.
	ZHANG C H, DING Y Y, WANG Y, et al. Association between polymorphism of prolactin receptor (PRLR) gene and litter size in Wei pigs and Dingyuan pigs[J]. Journal of China Agricultural University, 2011, 16(3): 117-121. (in Chinese with English abstract)
[20]	刘庆雨, 于永生, 金鑫, 等. 松辽黑猪和长白猪催乳素受体基因第10外显子多态性与繁殖性状的关联分析[J]. 中国畜牧兽医, 2012, 39(10): 191-195.
	LIU Q Y, YU Y S, JIN X, et al. Association analysis on polymorphisms of prolactin receptor (PRLR) gene exon 10 with reproductive traits in Songliao black pig and Landrace pig[J]. China Animal Husbandry & Veterinary Medicine, 2012, 39(10): 191-195. (in Chinese with English abstract)
[21]	陈文娟. 大白猪×清平猪杂交群体10个多态性位点与繁殖、生长和胴体性状的关联分析[D]. 武汉: 华中农业大学, 2018.
	CHEN W J. Correlation analysis of 10 polycondition point with reproductive, growth and carcass traits in hybrid population of Large white×Qingping[D]. Wuhan: Huazhong Agricultural University, 2018. (in Chinese with English abstract)
[22]	崔世泉, 李剑虹, 崔卫国, 等. 母猪哺乳初期的母性行为与催乳素受体基因多态性关系的初探[J]. 遗传, 2007, 29(1): 47-51.
	CUI S Q, LI J H, CUI W G, et al. Preliminary study on the relationship between sow maternal behaviour during early lactation and polymorphism of PRLR gene[J]. Hereditas, 2007, 29(1): 47-51. (in Chinese with English abstract)
[23]	GEORGESCU T, LADYMAN S R, BROWN R S E, et al. Acute effects of prolactin on hypothalamic prolactin receptor expressing neurones in the mouse[J]. Journal of Neuroendocrinology, 2020, 32(11): e12908.
[24]	SERROTE C M L, REINIGER L R S, SILVA K B, et al. Determining the polymorphism information content of a molecular marker[J]. Gene, 2020, 726: 144175. DOI URL
[25]	WIGGINTON J E, CUTLER D J, ABECASIS G R. A note on exact tests of hardy-Weinberg equilibrium[J]. The American Journal of Human Genetics, 2005, 76(5): 887-893. DOI URL
[26]	SUN Y X, ZENG Y Q, TANG H, et al. Relationship of genetic polymorphism of PRLR and RBP4 genes with litter size traits in pig[J]. Yi Chuan, 2009, 31(1): 63-68. DOI URL
[27]	VAN RENS B T, VAN DER LENDE T. Litter size and piglet traits of gilts with different prolactin receptor genotypes[J]. Theriogenology, 2002, 57(2): 883-893. PMID
[28]	VASHI Y, MAGOTRA A, KALITA D, et al. Evaluation of candidate genes related to litter traits in Indian pig breeds[J]. Reproduction in Domestic Animals, 2021, 56(4): 577-585. DOI PMID
[29]	范一萍, 王晓梅, 贾启涛, 等. 猪PRLR和FSHβ基因多态性检测及其与繁殖性状的关联分析[J]. 中国畜牧兽医, 2019, 46(3): 774-781.
	FAN Y P, WANG X M, JIA Q T, et al. Polymorphism of PRLR and FSHβ genes and its association analysis with reproductive traits in pigs[J]. China Animal Husbandry & Veterinary Medicine, 2019, 46(3): 774-781. (in Chinese with English abstract)
[30]	胡慧艳, 贾青, 赵思思, 等. 大白猪产仔数相关基因多态性及其遗传效应[J]. 中国兽医学报, 2017, 37(11): 2227-2234.
	HU H Y, JIA Q, ZHAO S S, et al. Polymorphisms and the genetic effects of genes with litter size in Large White pigs[J]. Chinese Journal of Veterinary Science, 2017, 37(11): 2227-2234. (in Chinese with English abstract)

引物名称 Primer name	正向引物 Forward primer (5'→3')	反向引物 Reverse primer (5'→3')	基因位置 Location in gene	产物长度 Product length/bp	退火温度 Tm/℃
P1	ACAGTCACCTCCGGGAAAAC	TTGTTGGGAGACACGCGAAT	g.20640695~g.20640852	158	59
P2	CTGGATCCCGACAGCGATTC	CTTGGATCCATCCGCGTGAA	g.20655030~g.20655245	216	60
P3	CCCTAGGCCACTCACTTGCT	AATTAGTCCTGGGAGGGCTGT	g.20648455~g.20649007	553	58
P4	ATGATCTGGGCAGTGGCTTT	TGCTGGCACTTACCTCCAAC	g.20652417~g.20653041	625	60

引物名称 Primer name	正向引物 Forward primer (5'→3')	反向引物 Reverse primer (5'→3')	基因位置 Location in gene	产物长度 Product length/bp	退火温度 Tm/℃
P1	ACAGTCACCTCCGGGAAAAC	TTGTTGGGAGACACGCGAAT	g.20640695~g.20640852	158	59
P2	CTGGATCCCGACAGCGATTC	CTTGGATCCATCCGCGTGAA	g.20655030~g.20655245	216	60
P3	CCCTAGGCCACTCACTTGCT	AATTAGTCCTGGGAGGGCTGT	g.20648455~g.20649007	553	58
P4	ATGATCTGGGCAGTGGCTTT	TGCTGGCACTTACCTCCAAC	g.20652417~g.20653041	625	60

SNP位点 SNP loci	基因型(频率,样本数) Genotype(frequency, sample number))			等位基因(频率) Allele(frequency)		He	Ne	PIC	χ²
g.20655220 C>T	CC (0.583,119)	CT (0.358,73)	TT (0.059,12)	C(0.762)	T(0.238)	0.362	1.569	0.297	0.033
g.20648859 C>T	CC (0.657,134)	CT (0.255,52)	TT (0.088,18)	C(0.784)	T(0.216)	0.338	1.511	0.281	12.405
g.20648931 C>T	CC (0.755,154)	CT (0.196,40)	TT (0.049,10)	C(0.853)	T(0.147)	0.251	1.335	0.219	9.730

SNP位点 SNP loci	基因型(频率,样本数) Genotype(frequency, sample number))			等位基因(频率) Allele(frequency)		He	Ne	PIC	χ²
g.20655220 C>T	CC (0.583,119)	CT (0.358,73)	TT (0.059,12)	C(0.762)	T(0.238)	0.362	1.569	0.297	0.033
g.20648859 C>T	CC (0.657,134)	CT (0.255,52)	TT (0.088,18)	C(0.784)	T(0.216)	0.338	1.511	0.281	12.405
g.20648931 C>T	CC (0.755,154)	CT (0.196,40)	TT (0.049,10)	C(0.853)	T(0.147)	0.251	1.335	0.219	9.730

倍型 Ploidy	SNP位点(样本数) SNP locus(Sample number)	g.20655220 C>T	g.20648859 C>T	g.20648931 C>T	频率 Frequency/%
单倍型	H1(243)	C	C	C	0.610
Haplotypes	H2(28)	C	T	C	0.069
	H3(40)	C	T	T	0.098
	H4(77)	T	C	C	0.189
	H5(20)	T	T	T	0.049
双倍型	H1H1(78)	C C	C C	C C	0.382
Diplotypes	H1H2(11)	C C	C T	C C	0.054
	H1H3(22)	C C	C T	C T	0.108
	H1H4(44)	C T	C C	C C	0.216
	H1H5(10)	C T	T T	T T	0.049
	H2H3(8)	T T	T T	T T	0.039
	H2H4(9)	C T	C T	C C	0.044
	H3H5(10)	C T	T T	T T	0.049
	H4H4(12)	T T	C C	C C	0.059

柯乐猪PRLR基因多态性与繁殖性状的关联性

Correlation of PRLR gene polymorphisms and reproductive traits in Kele pig

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 9

参考文献 30

相关文章 15

编辑推荐

Metrics

本文评价

SNP位点 SNP loci	g.20655220 C>T	g.20648859 C>T	g.20648931 C>T
g.20655220 C>T		0.111	0.022
g.20648859 C>T	0.001		1.000
g.20648931 C>T	0	0.627

位点 Locus	基因型 Genotype	每窝总产仔数 Total number born	每窝产活仔数 Number born alive	初生窝重 Newborn litter weight/kg	初生个体重 Individual birth weight/kg	每窝断奶仔猪数 Number of weaned piglets	断奶窝重 Weight of weaning litter/kg	断奶个体重 Weaning individual weight/kg
g.20655220	CC(119)	8.765±2.547	8.050±2.587	8.785±3.018	1.109±0.229 a	6.967±2.278	39.551±13.000 a	5.776±1.277 a
C>T	CT(73)	8.671±2.582	7.726±2.864	7.950±3.090	1.044±0.205 b	6.616±2.542	35.423±13.395 b	5.417±0.817 b
	TT(12)	9.500±2.468	8.417±3.089	9.283±4.229	1.086±0.209 ab	6.833±2.362	36.600±10.525 ab	5.443±0.589 ab
g.20648859	CC(134)	8.716±2.530	7.948±2.640	8.407±2.993	1.073±0.214	6.828±2.306	37.490±12.621	5.567±0.961
C>T	CT(52)	8.769±2.478	8.019±2.797	8.927±3.417	1.125±0.233	6.846±2.321	38.747±13.351	5.724±1.295
	TT(18)	9.222±2.981	7.833±3.130	8.131±3.416	1.053±0.231	6.833±2.975	38.508±16.323	5.809±1.545
g.20648931	CC(154)	8.617±2.487	7.857±2.631	8.327±2.987 b	1.076±0.221 b	6.747±2.288	37.144±12.757 b	5.570±1.010
C>T	CT(40)	9.225±2.636	8.425±2.782	9.684±3.440 a	1.158±0.207 a	7.300±2.3345	42.326±13.089 a	5.930±1.466
	TT(10)	9.400±3.098	7.600±3.658	6.740±2.970 b	0.916±0.145 c	6.300±3.401	31.845±15.107 b	5.311±0.781

双倍型 Diplotype	每窝总产仔数 Total number born	每窝产活仔数 Number born alive	初生窝重 Newborn litter weight/kg	初生个体重 Individual birth weight/kg	每窝断奶仔猪数 Number of weaned piglets	断奶窝重 Weight of weaning litter/kg	断奶个体重 Weaning individual weight/kg
H1H1(78)	8.731±2.567 ab	8.051±2.613	8.586±2.980 abc	1.084±0.223 abc	6.936±2.275	38.482±12.672 abc	5.635±1.095 ab
H1H2(11)	8.364±2.248 ab	7.364±2.942	7.809±3.263 abc	1.091±0.310 abc	6.091±2.300	35.455±14.903 abc	5.836±1.527 ab
H1H3(22)	9.000±2.582 ab	8.364±2.441	9.587±2.859 a	1.165±0.204 a	7.318±2.255	42.739±11.863 ab	6.011±1.397 ab
H1H4(44)	8.477±2.492 ab	7.636±2.589	7.853±2.576 bc	1.050±0.201 bc	6.636±2.441	35.973±13.129 bc	5.480±0.775 b
H1H5(10)	9.900±2.601 a	8.800±3.795	9.750±4.875 ab	1.091±0.217 abc	7.100±2.726	37.810±14.445 abc	5.351±0.934 b
H2H3(8)	9.000±3.024 ab	8.125±2.532	9.869±3.284 ab	1.224±0.206 a	7.500±2.390	46.838±14.531 a	6.433±2.054 a
H2H4(9)	7.444±1.944 b	7.111±2.147	7.767±2.763 abc	1.108±0.238 abc	6.333±2.062	34.056±13.041 b	5.302±1.029 b
H3H5(10)	9.400±3.098 ab	7.600±3.658	6.740±2.969 b	0.916±0.145 b	6.300±3.41	31.845±15.103 bc	5.311±0.781 b
H4H4(12)	9.500±2.468 ab	8.417±3.088	9.283±4.229 abc	1.086±0.210 abc	6.833±2.125	36.600±10.525 bc	5.443±0.589 ab

[1]	孙仁杰, 徐慧玲, 孙思琪, 柴娟, 虞一聪, 谢荣辉, 李肖梁, 赵灵燕, 张传亮. 非洲猪瘟病毒基因Ⅰ型和Ⅱ型PCR-RFLP鉴别方法的建立[J]. 浙江农业学报, 2025, 37(5): 1017-1028.
[2]	朱艳宇, 于文涛, 高水练, 吕水源, 王攀, 靳宛旻, 贵文静, 林浥, 叶乃兴. 福建安溪茶树种质资源遗传多样性与铁观音衍生品种遗传关系[J]. 浙江农业学报, 2024, 36(7): 1591-1601.
[3]	向进, 王春源, 吴燕, 谭元成, 杨酸, 张依裕. 柯乐猪CRISP3基因SNP鉴定及其对繁殖性状的影响[J]. 浙江农业学报, 2024, 36(6): 1270-1278.
[4]	马丽娜, 田进阳, 王锦, 赵正伟, 马青. 滩羊FGF5、COQ9基因多态性及其与生长性状的关联分析[J]. 浙江农业学报, 2024, 36(5): 1015-1023.
[5]	董飚, 纪荣超, 张干生, 王健. 番鸭A-FABP基因外显子2多态性及其与生长性能和肉品质的关联分析[J]. 浙江农业学报, 2024, 36(11): 2456-2464.
[6]	袁晔, 刘睿, 王凌云, 沈盟, 叶雪莲, 权新华, 王瑞森, 姚祥坦. 江浙地区菱品种遗传多样性的SLAF-seq分析[J]. 浙江农业学报, 2023, 35(8): 1773-1781.
[7]	聂玮, 孟科, 荣轩, 强浩, 郭晨浩, 陶毛孩, 冯登侦. 绵羊GRM1基因多态性及其与肉质性状的相关性[J]. 浙江农业学报, 2023, 35(4): 799-808.
[8]	王龙威, 白俊艳, 贾小平, 雷莹, 陈梦柯, 樊红灯, 卢小宁, 何豫涵, 曾凡林, 张容恺. 鹌鹑GnRH-1基因多态性与蛋品质的关联分析[J]. 浙江农业学报, 2023, 35(3): 565-574.
[9]	葛健辉, 关文志, 任晋东, 牛宝龙, 胡金春, 王伟, 翁旭东, 楼宝, 于瑾, 许晓军. 马口鱼全基因组简单重复序列特征分析与多态性标记开发[J]. 浙江农业学报, 2023, 35(11): 2584-2593.
[10]	刘鹏程, 张继, 邱淦远, 龚俞, 李雪松, 李维, 张依裕, 刘若余. 关岭牛TBC1D7基因单核苷酸多态性筛查及生物信息学分析[J]. 浙江农业学报, 2022, 34(7): 1402-1411.
[11]	王新乐, 白俊艳, 李静云, 雷莹, 李淦, 庞有志, 董智豪, 陈宇, 樊红灯, 王龙威, 陈梦柯, 曾凡林, 安肖凯, 白永振. 肉用鹌鹑血管活性肠肽Ⅰ型受体基因的多态性与胴体性状的关联分析[J]. 浙江农业学报, 2022, 34(11): 2379-2385.
[12]	许金根, 靳二辉, 王重龙, 顾有方, 李庆岗. 猪CAST基因多态性与生物信息学分析[J]. 浙江农业学报, 2022, 34(1): 17-23.
[13]	董智豪, 陈宇, 黄高想, 白俊艳, 李静云, 赵淑娟, 雷莹, 王新乐, 胡琦杭, 范征宇. 蛋用鹌鹑的VIPR-1基因的多态性与早期生长性状的关联分析[J]. 浙江农业学报, 2021, 33(8): 1393-1401.
[14]	林朦婕, 温慧萍, 肖建中, 郑强. 四十八份青花菜品种SSR指纹图谱构建[J]. 浙江农业学报, 2021, 33(12): 2304-2312.
[15]	陶鹏, 岳智臣, 赵彦婷, 雷娟利, 李必元. 大白菜BrSPS1Fb基因剪接受体位点变异及其对剪接的影响[J]. 浙江农业学报, 2021, 33(11): 2068-2074.