杭州地区草地贪夜蛾田间动态分析与生物型鉴定

doi:10.3969/j.issn.1004-1524.2023.05.15

浙江农业学报 ›› 2023, Vol. 35 ›› Issue (5): 1116-1122.DOI: 10.3969/j.issn.1004-1524.2023.05.15

杭州地区草地贪夜蛾田间动态分析与生物型鉴定

王国迪¹(), 冯行利²^,³, 吴涛起², 汪彦欣¹, 吴慧明², 饶琼², 张莉丽¹^,^*()

1.杭州市农业技术推广中心,浙江杭州 310020
2.浙江农林大学现代农学院浙江省农产品品质改良技术研究重点试验室,浙江杭州 311300
3.金华市恒安生物技术有限公司,浙江金华 321000

收稿日期:2022-09-06 出版日期:2023-05-25 发布日期:2023-06-01
作者简介:王国迪(1972—),男,浙江绍兴人,学士,高级农艺师,主要从事农业有害生物监测和防治工作。E-mail:wangguodi@126.com
通讯作者: *张莉丽,E-mail:lilyzh_hz@126.com
基金资助:
杭州市科技发展项目(20201203B160)

Field population dynamics analysis and biotype identification of Spodoptera frugiperda in Hangzhou, China

WANG Guodi¹(), FENG Hangli²^,³, WU Taoqi², WANG Yanxin¹, WU Huiming², RAO Qiong², ZHANG Lili¹^,^*()

1. Hangzhou Agricultural Technology Extension Center, Hangzhou 310020, China
2. Zhejiang Provincial Key Laboratory for Agricultural Product Quality Improvement Technology Research, College of Advanced Agricultural Sciences, Zhejiang A & F University, Hangzhou 311300, China
3. Jinhua Heng’an Biotechnology Co., Ltd., Jinhua 321000, Zhejiang, China

Received:2022-09-06 Online:2023-05-25 Published:2023-06-01

摘要/Abstract

摘要：

草地贪夜蛾(Spodoptera frugiperda)自2019年1月入侵我国以来,已在我国多个省份暴发,对农业生产造成巨大威胁。为了解草地贪夜蛾在浙江杭州地区的田间分布及发生规律,采用田间诱捕法,在杭州不同玉米种植区设置性诱监测点,2019—2021年连续3年对草地贪夜蛾成虫进行监测,并通过分子生物学方法对其生物型进行鉴定。结果表明,经分子鉴定杭州地区草地贪夜蛾是玉米型和水稻型杂交种,其取食偏好玉米叶片。连续监测结果显示,2019年下半年杭州地区草地贪夜蛾发生最为严重,单个诱捕器每7 d最多可诱集到388头;2020年和2021年草地贪夜蛾的发生逐年下降,单个诱捕器每7 d最多分别可诱集到174头和29头。

关键词: 草地贪夜蛾, 生物型, 性诱监测, 发生动态, 杭州

Abstract:

The armyworm Spodoptera frugiperda, which invaded China in January 2019, has been an outbreak in many provinces of China, causing a great impact on food security production. In order to determine the distribution and occurrence regularity of S. frugiperda in the field of Hangzhou, Zhejiang Province, monitoring sites were set up to monitor the population of S. frugiperda in different maize planting areas from 2019 to 2021, and the biotypes of S. frugiperda were identified by molecular biological methods. The results showed that S. frugiperda mainly distributed in Hangzhou area was identified as a hybrid of maize-type and rice-type by molecular identification, and its feeding preference was maize leaves. In 2019, S. frugiperda infestations occurred in many areas in Hangzhou, especially in September to November. The highest number of trapped male moths in a single trap was 388 moths per week. The occurrence of S. frugiperda in Hangzhou decreased significantly in 2020 and 2021. The highest number of trapped male moths in a single trap was 174 moths per week and 29 moths per week respectively.

Key words: Spodoptera frugiperda, biotype, field trapping, occurrence dynamics, Hangzhou

中图分类号:

王国迪, 冯行利, 吴涛起, 汪彦欣, 吴慧明, 饶琼, 张莉丽. 杭州地区草地贪夜蛾田间动态分析与生物型鉴定[J]. 浙江农业学报, 2023, 35(5): 1116-1122.

WANG Guodi, FENG Hangli, WU Taoqi, WANG Yanxin, WU Huiming, RAO Qiong, ZHANG Lili. Field population dynamics analysis and biotype identification of Spodoptera frugiperda in Hangzhou, China[J]. Acta Agriculturae Zhejiangensis, 2023, 35(5): 1116-1122.

图/表 4

图1 2019—2021年杭州地区草地贪夜蛾田间诱捕量热图(单位:头/诱捕器)

Fig.1 Dynamics of trapped S. frugiperda in the field in Hangzhou from 2019 to 2021 (Unit: male moths/trap)

图2 基于COI基因序列的草地贪夜蛾系统发育分析 RS为水稻型、CS为玉米型、HZ strain1和HZ strain2为本试验种群、Bombyx mori为外群。

Fig.2 Phylogenetic analysis of S. frugiperda based on COI gene sequences RS is a rice type, CS is a maize type, HZ strain1 and HZ strain2 are the experimental populations, and Bombyx mori is the outgroup.

图3 基于Tpi基因片段单倍型位点比较图中HZ-Strain1和HZ-Strain2为本试验样本;红色为玉米型和水稻型基因差异位点。

Fig.3 Haplotype loci analysis based on Tpi gene fragments HZ-Strain1 and HZ-Strain2 in the figure are samples for this experiment; The red arrow indicates the genetic differences between maize and rice types.

图4 草地贪夜蛾幼虫对两种寄主植物的取食选择图中数据是平均值±标准误;**代表两者差异性显著。

Fig.4 Feeding rate of Spodoptera frugiperda on two host plants The data in the figure is the average ± standard error. ** represents significant differences between the two.

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杭州地区草地贪夜蛾田间动态分析与生物型鉴定

Field population dynamics analysis and biotype identification of Spodoptera frugiperda in Hangzhou, China

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