玉米大斑病菌醇脱氢酶基因家族的鉴定和生物信息学分析

doi:10.3969/j.issn.1004-1524.2023.05.14

浙江农业学报 ›› 2023, Vol. 35 ›› Issue (5): 1108-1115.DOI: 10.3969/j.issn.1004-1524.2023.05.14

玉米大斑病菌醇脱氢酶基因家族的鉴定和生物信息学分析

张淑红(), 张运峰, 武秋颖, 高凤菊, 李亚子, 纪景欣, 许可, 范永山()

唐山师范学院生命科学系,唐山市农业病原真菌与毒素重点实验室,河北唐山 063000

收稿日期:2022-01-06 出版日期:2023-05-25 发布日期:2023-06-01
作者简介:张淑红(1978—),女,河北赞皇人,硕士,副教授,主要从事微生物遗传研究。E-mail:zhangshuhong@tstc.edu.cn
通讯作者: *范永山,E-mail:fanyongshan@126.com
基金资助:
唐山市人才项目(A202007009);唐山师范学院科研项目(2021B30);唐山师范学院科研项目(2021B34);唐山师范学院重点培育项目(ZDPY06);唐山师范学院重点培育项目(ZDPY08)

Identification and bioinformatics analysis of alcohol dehydrogenase gene family of Setosphaeria turcica

ZHANG Shuhong(), ZHANG Yunfeng, WU Qiuying, GAO Fengju, LI Yazi, JI Jingxin, XU Ke, FAN Yongshan()

Department of Life Sciences, Tangshan Normal University, Tangshan Key Laboratory of Agricultural Pathogenic Fungi and Toxins, Tangshan 063000, Hebei, China

Received:2022-01-06 Online:2023-05-25 Published:2023-06-01

摘要/Abstract

摘要：

醇脱氢酶(alcohol dehydrogenase,ADH)是生物体内主要短链醇代谢的关键酶,在物质代谢和能量转化过程中发挥着重要作用。本文从玉米大斑病菌(Setosphaeria turcica)基因组的KEGG数据库中获得了22个ADH基因家族成员(StADH1-StADH22),它们散布在12条scaffold上,长232~495 aa,理论等电点5.65~9.22,81.8%家族成员呈酸性,72.7%为亲水蛋白,有4个家族成员定位于线粒体,2个定位于过氧化物酶体,其余16个家族成员均定位于细胞质。系统发育分析结果表明,有11个玉米大斑病菌ADH家族蛋白与酵母ADH(YADH1-7)亲缘关系较近,含有相似的保守基序和结构域,其他11个玉米大斑病菌ADH家族蛋白与酵母ADH亲缘关系较远,含有不同的保守基序和结构域。玉米大斑病菌侵染玉米叶片的转录组分析结果表明,仅有4个ADH基因(StADH1、StADH4、StADH6和StADH12)参与病菌侵染过程,其中StADH1、StADH4和StADH12主要在病菌侵染后期发挥作用,而StADH1还可能与病菌的致病专化性有关。

关键词: 玉米大斑病菌, 醇脱氢酶(ADH), 基因家族, 转录组, 致病性

Abstract:

Alcohol dehydrogenase (ADH) is a major enzyme of short chain alcohol metabolism in all organisms and plays an important role in the process of substance metabolism and energy conversion. Utilizing the KEGG database of Setosphaeria turcica genome, 22 members of the ADH gene family (StADH1-StADH22) of Setosphaeria turcica were obtained in this paper. The ADH genes were distributed over 12 scaffolds with 232-495 aa in length, the theoretical isoelectric point of 5.65-9.22, 81.8% acidic family members and 72.7% hydrophilic proteins. Four family members were located in mitochondria, 2 members in peroxisome, and the other 16 family members were located in cytoplasm. Phylogenetic analysis results show that there are 11 Setosphaeria turcica ADH family proteins closely related with yeast ADH gens (YADH1-7) which have the similar conservative motifs and structure domains, and the other 11 StADH family proteins are far from yeast ADH and contain different conservative motifs and structure domains. Through the analysis of Setosphaeria turcica-Zea mays gene expression transcriptome, there are four StADH family genes StADH1, StADH4, StADH6 and StADH12 participate in the pathogen infection process, and StADH1, StADH4 and StADH12 play very important roles in the later period of infection. Furthermore, StADH1 might be related to the pathogenicity specificity of the pathogen.

Key words: Setosphaeria turcica, alcohol dehydrogenase, gene family, transcriptome, pathogenicity

中图分类号:

S432.1

张淑红, 张运峰, 武秋颖, 高凤菊, 李亚子, 纪景欣, 许可, 范永山. 玉米大斑病菌醇脱氢酶基因家族的鉴定和生物信息学分析[J]. 浙江农业学报, 2023, 35(5): 1108-1115.

ZHANG Shuhong, ZHANG Yunfeng, WU Qiuying, GAO Fengju, LI Yazi, JI Jingxin, XU Ke, FAN Yongshan. Identification and bioinformatics analysis of alcohol dehydrogenase gene family of Setosphaeria turcica[J]. Acta Agriculturae Zhejiangensis, 2023, 35(5): 1108-1115.

图/表 5

图1 玉米大斑病菌ADH基因染色体定位

Fig.1 Chromosome locations of ADH genes in Setosphaeria turcica

图2 玉米大斑病菌ADH的系统发育树

Fig.2 Phylogenetic tree of the ADH of Setosphaeria turcica

图3 玉米大斑病菌ADH基因在接种后不同时间的相对表达量分析

Fig.3 Analysis of the relative expression of ADH gene in Setosphaeria turcica at different time after inoculation

图4 玉米大斑病菌ADH基因家庭成员在病菌侵染后的表达规律 A,接种抗病自交系(R);B,接种感病自交系(S);C,接种10 d的基因表达差异;*表示差异显著(P<0.05)。

Fig.4 Expression of family members of ADH gene in Setosphaeria turcica after infection A,Inoculated disease-resistant inbred line (R); B,Inoculated susceptible inbred line (S); C,Difference of gene expression after 10 days of inoculation; * Indicating the significant difference(P<0.05).

图5 玉米大斑病菌ADH基因家族关键基因的结构域分析

Fig.5 Domain analysis of key genes of ADH gene family in Setosphaeria turcica

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玉米大斑病菌醇脱氢酶基因家族的鉴定和生物信息学分析

Identification and bioinformatics analysis of alcohol dehydrogenase gene family of Setosphaeria turcica

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