山茱萸提取物缓解小鼠急性肾损伤的功能活性评价

doi:10.3969/j.issn.1004-1524.20250568

浙江农业学报 ›› 2026, Vol. 38 ›› Issue (5): 876-885.DOI: 10.3969/j.issn.1004-1524.20250568

山茱萸提取物缓解小鼠急性肾损伤的功能活性评价

江豪¹(), 周万怡², 陶文扬², 王梦竹², 邢建荣², 杨颖²^,^*(), 曾垂省¹^,^*()

¹ 重庆邮电大学生命健康信息科学与工程学院, 重庆 400065
² 浙江省农业科学院食品科学研究所, 浙江杭州 310021

收稿日期:2025-09-04 出版日期:2026-05-25 发布日期:2026-06-02
作者简介:江豪,研究方向为天然产物功效挖掘。E-mail:1779051304@qq.com
通讯作者: *杨颖,E-mail:YangYing_SPS@163.com;曾垂省,E-mail:zengcs@cqupt.edu.cn
基金资助:
浙江省“尖兵领雁+X”科技计划(2025C01101)

Evaluation of the functional activity of Cornus officinalis extract in alleviating acute kidney injury in mice

JIANG Hao¹(), ZHOU Wanyi², TAO Wenyang², WANG Mengzhu², XING Jianrong², YANG Ying²^,^*(), ZENG Chuisheng¹^,^*()

¹ School of Life and Health Information Sciences and Engineering, Chongqing University of Posts and Telecommunications, Chongqing 400065, China
² Institute of Food Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2025-09-04 Published:2026-05-25 Online:2026-06-02

摘要/Abstract

摘要：

为探究山茱萸提取物缓解急性肾损伤的潜在机制,本研究选取C57BL/6J雄性小鼠,通过腹腔注射庆大霉素建立小鼠急性肾损伤模型,将小鼠分为正常对照组、模型组及山茱萸提取物干预组,分别检测小鼠体重、脏器系数、血清肌酐与尿素氮浓度、炎症因子质量浓度,观察肾脏组织病理变化,并分析肠道菌群组成。结果显示,与模型组相比,山茱萸提取物组小鼠体重变化率有所下降;血清肌酐和尿素氮浓度显著降低;IL-6与TNF-α质量浓度显著下降,IL-10质量浓度显著升高;肾小管上皮细胞坏死、炎症浸润及纤维化等病理损伤明显缓解;肠道中有益菌丰度增加、有害菌丰度减少。结果表明,山茱萸提取物可通过调节炎症反应及改善肠道微生态,对急性肾损伤发挥保护作用。本研究从组织学、免疫学和微生态学角度阐明了山茱萸提取物对急性肾损伤的影响,为山茱萸提取物的功能评价与精准应用提供了参考。

关键词: 山茱萸提取物, 急性肾损伤, 肾功能, 炎症因子, 肠道菌群

Abstract:

To investigate the potential mechanism by which Cornus officinalis extract alleviates acute kidney injury, male C57BL/6J mice were selected. An acute kidney injury model was established in mice via intraperitoneal injection of gentamicin. The mice were divided into a normal control group, a model group, and a Cornus officinalis extract intervention group. Body weight, organ coefficients, serum creatinine and urea nitrogen concentrations, and inflammatory cytokine mass concentrations were measured. Renal histopathological changes were observed by tissue sectioning, and the composition of the intestinal flora was analyzed. The results showed that, compared with the model group, the rate of body weight change was lower in the Cornus officinalis extract group; serum creatinine and urea nitrogen concentrations were significantly reduced; IL-6 and TNF-α mass concentrations were significantly decreased, while IL-10 mass concentration was significantly increased; pathological injuries such as renal tubular epithelial cell necrosis, inflammatory infiltration, and fibrosis were significantly alleviated; the abundance of beneficial bacteria in the intestine increased, while the abundance of harmful bacteria decreased. The results indicate that Cornus officinalis extract can exert a protective effect on acute kidney injury by regulating inflammatory responses and improving the intestinal microecology. This study elucidates the effects of Cornus officinalis extract on acute kidney injury from histological, immunological, and microbiological perspectives, providing a reference for the functional evaluation and precise application of Cornus officinalis extract.

Key words: Cornus officinalis extract, acute kidney injury, renal function, inflammatory factor, gut microbiota

中图分类号:

R285.5

江豪, 周万怡, 陶文扬, 王梦竹, 邢建荣, 杨颖, 曾垂省. 山茱萸提取物缓解小鼠急性肾损伤的功能活性评价[J]. 浙江农业学报, 2026, 38(5): 876-885.

JIANG Hao, ZHOU Wanyi, TAO Wenyang, WANG Mengzhu, XING Jianrong, YANG Ying, ZENG Chuisheng. Evaluation of the functional activity of Cornus officinalis extract in alleviating acute kidney injury in mice[J]. Acta Agriculturae Zhejiangensis, 2026, 38(5): 876-885.

图/表 5

图1 山茱萸提取物对AKI小鼠脏器系数和体重变化率的影响 NC,空白对照组;MG,模型组;CWE,山茱萸提取物组。柱上无相同小写字母表示差异显著(p<0.05)。下同。

Fig.1 Effects of Cornus officinalis extract on organ index and body weight change rate in AKI mice NC, Normal control group; MG, Model group; CWE, Cornus officinalis extract treatment group. Different lowercase letters on the bars indicate significant (p<0.05) differences. The same as below.

图2 山茱萸提取物对AKI小鼠血清肌酐和尿素氮浓度的影响与NC组比较,##表示p<0.01;与MG比较,*表示p<0.05,***表示p<0.001。

Fig.2 Effects of Cornus officinalis extract on serum creatinine (Scr) and blood urea nitrogen (BUN) concentrations in AKI mice Compared with the NC group, ## indicates p<0.01; Compared with the MG group, * indicates p<0.05, *** indicates p<0.001.

图3 山茱萸提取物对AKI小鼠炎症因子质量浓度的影响与NC组比较,##表示p<0.01,####表示p<0.000 1;与MG比较,**表示p<0.01,***表示p<0.001。

Fig.3 Effects of Cornus officinalis extract on inflammatory cytokine mass concentrations in AKI mice Compared with the NC group, ## indicates p<0.01, #### indicates p<0.000 1; compared with the MG group, ** indicates p<0.01, *** indicates p<0.001.

图4 山茱萸提取物对AKI小鼠肾脏组织病理学的影响红色箭头,炎性细胞;黑色箭头,肾小管上皮细胞坏死。

Fig.4 Effects of Cornus officinalis extract on renal histopathology in AKI mice Red arrows, Inflammatory cells; Black arrows, Necrosis of renal tubular epithelial cells.

图5 山茱萸提取物对AKI小鼠肠道菌群的影响 A,物种Venn图分析;B,Chao 1指数;C,Shannon指数;D,主成分分析(PCA);E,主坐标分析(PCoA);F,门水平相对丰度;G,属水平相对丰度;H,多组比较分析;I,LEfSe分析;J,相关分析。PC1,第1主成分;PC2,第2主成分;PCoA1,第1主坐标;PCoA2,第2主坐标。LDA值已转换为常用对数值。

Fig.5 Effects of Cornus officinalis extract on gut microbiota in AKI mice A, Venn diagram analysis of species; B, Chao 1 index; C, Shannon index; D, Principal component analysis (PCA); E, Principal coordinates analysis (PCoA); F, Relative abundance at the phylum level; G, Relative abundance at the genus level; H, Multi-group comparison analysis; I, LEfSe analysis; J, Correlation analysis. PC1, Principal component 1; PC2, Principal component 2; PCoA1, Principal coordinate 1; PCoA1 Principal coordinate 2. The LDA score is converted into common logarithmic value.

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山茱萸提取物缓解小鼠急性肾损伤的功能活性评价

Evaluation of the functional activity of Cornus officinalis extract in alleviating acute kidney injury in mice

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