浙江农业学报 ›› 2026, Vol. 38 ›› Issue (5): 886-897.DOI: 10.3969/j.issn.1004-1524.20250509

• 园艺科学 • 上一篇    下一篇

茭白种质资源收集及不同茭白品种对茭白胡麻斑病的抗性评价

胡江龙1(), 姜万龙2, 彭辉1, 吴骏成2, 张真3, 汤近天1, 张雅芬1, 叶子弘1,2,*()   

  1. 1 中国计量大学 生命科学学院, 浙江省生物计量与检验检疫技术重点实验室, 浙江 杭州 310018
    2 中国计量大学 现代科技学院, 浙江 金华 322002
    3 桐乡市乌镇镇农业经济服务中心, 浙江 桐乡 314501
  • 收稿日期:2025-07-26 出版日期:2026-05-25 发布日期:2026-06-02
  • 作者简介:胡江龙,主要从事菰黑粉菌与茭白互作研究。E-mail:1269112627@qq.com
  • 通讯作者: *叶子弘,E-mail:zhye@cjlu.edu.cn
  • 基金资助:
    国家自然科学基金联合基金(U20A2043)

Collection of Zizania latifolia germplasm resources and evaluation of resistance to leaf spot disease in different Zizania latifolia germplasm

HU Jianglong1(), JIANG Wanlong2, PENG Hui1, WU Juncheng2, ZHANG Zhen3, TANG Jintian1, ZHANG Yafen1, YE Zihong1,2,*()   

  1. 1 Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang University, Hangzhou 310018, China
    2 College of Modern Science and Technology, China Jiliang University, Jinhua 322002, Zhejiang, China
    3 Wuzhen Town Agricultural Economic Service Center of Tongxiang City, Tongxiang 314501, Zhejiang, China
  • Received:2025-07-26 Published:2026-05-25 Online:2026-06-02

摘要:

为研究不同品种茭白的熟性、茭型,以及茭白胡麻斑病抗性等关键性状,本研究收集了来自13个省(市)的116个茭白品种,对各品种的熟性及肉质茎形态进行了系统调查,同步对各品种的内生菰黑粉菌进行分离及形态学鉴定。此外,通过人工接种茭白胡麻斑病生理小种LY-H1后的病情指数分级评价,鉴定各品种对茭白胡麻斑病的抗性。结果表明:在收集的种质中,双季茭53种、单季茭63种,熟性分布以中熟型为主,双季茭多呈圆锥形,单季茭则多为长条形。同时成功分离出103株内生菰黑粉菌,其菌落形态主要分为致密菌丝型和孢子型,致密菌丝型存在卷曲生长型、贴附生长型和隆起生长型3种形态,孢子型存在颗粒型和褶皱型2种形态。此外,单季茭对茭白胡麻斑病的抗性普遍强于双季茭。研究结果为茭白种质资源的收集保存、评价及抗病育种提供了重要的材料基础和科学依据。

关键词: 茭白, 菰黑粉菌, 种质资源, 熟性, 抗病性

Abstract:

To investigate key traits such as maturity, type, and resistance to leaf spot disease in different cultivars of water bamboo (Zizania latifolia), this study collected 116 water bamboo varieties from 13 provinces (municipalities) in China. Systematic investigations were conducted on the maturity and fleshy stem morphology of each variety, alongside the isolation and morphological identification of their endophytic Ustilago esculenta. Furthermore, resistance to water bamboo leaf spot (Bipolaris oryzae) was evaluated through artificial inoculation with physiological race LY-H1 and subsequent disease index rating. The results indicated that among the collected germplasm, there were 53 double-harvest varieties and 63 single-harvest varieties. Mid-maturing types were predominant in both groups. Double-harvest varieties primarily exhibited conical stems, whereas single-harvest varieties were mostly elongated. Meanwhile, 103 endophytic U. esculenta strains were successfully isolated, with colony morphologies primarily classified into dense mycelium-type and spore-type. The dense mycelium-type exhibited three morphologies: curly growth type, adherent growth type, and elevated growth type, while the spore-type exhibited two morphologies: granular type and wrinkled type.Additionally, single-harvest varieties generally demonstrated stronger resistance to B. oryzae than double-harvest varieties. This study provides valuable germplasm materials and a scientific foundation for the collection, conservation, evaluation, and disease-resistant breeding of water bamboo resources.

Key words: Zizania latifolia, Ustilago esculenta, germplasm resource, maturity, disease resistance

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