›› 2011, Vol. 23 ›› Issue (4): 0-691.

• 动物科学 •    

家蚕磷脂酰肌醇3-激酶(Bmpi3k)基因的克隆及分析

庄俐1,2,牛宝龙2,张静静1,2,孟智启2,*   

  1. 1浙江师范大学 化学与生命科学学院, 浙江 金华 321004;2 浙江省农业科学院 蚕桑研究所, 浙江 杭州 310021
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2011-07-25 发布日期:2011-07-25

Cloning of the phosphoinositide 3-kinase gene from silkworm (Bombyx mori)

ZHUANG Li;NIU Bao-long;ZHANG Jing-jing;MENG Zhi-qi;*   

  1. 1College of Chemistry and life Sciences,Zhejiang Normal University, Jinhua 321004, China;2Seticulturn Research Institute, Zhejiang Academy of Agricultural Sciences,Hangzhou 310021, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-07-25 Published:2011-07-25

摘要: 磷脂酰肌醇3-激酶是一种重要的信号传导因子,对细胞生长、调节等生命活动起重要的作用。文章通过对家蚕(Bombyx mori)表达序列标签(EST)搜索及基因组DNA的预测,利用RT-PCR克隆得到家蚕磷脂酰肌醇3-激酶基因的全长cDNA序列,命名为Bmpi3k。序列分析表明,该基因mRNA全长3 368 bp,其开放式阅读框(ORF)大小为3 165 bp,编码产生1055个氨基酸。其预测的蛋白分子量为122.79 kDa,等电点为7.32。通过对BmPI3K蛋白质序列比对分析发现,BmPI3K保守性较高,包含5个PI3K蛋白特有的保守结构域,推测其参与了家蚕的重要生命活动的调节。

关键词: 家蚕, Bmpi3k, 电子克隆, 表达序列标签(EST)

Abstract: Phosphatidylinositol 3-kinase, as one of important signal transduction factors, plays an important role in cell growth and regulation activities. Silkworm phosphatidylinositol 3-kinase gene, named as Bmpi3k, was cloned by searching the silkworm (Bombyx mori) expressed sequence tags (EST), genomic DNA prediction, and RT-PCR. Its mRNA was 3 368 bp in full-length with an open reading frame (ORF) of 3 165 bp encoding a protein of 1 055 amino acids. The protein molecular weight was 122.79 kDa with the isoelectric point of 7.32. It contains five specific conserved domains of PI3K protein. The results suggested that BmPI3K was involved in a major life activity of silkworm.

Key words: Bombyx mori, Bmpi3k, silico cloning, expression sequence tag (EST)