›› 2011, Vol. 23 ›› Issue (5): 0-879.

• 作物科学 •    

马铃薯脱毒试管苗保苗与试管薯诱导条件优化

刘艳芬,陈翠果,梁伟玲,暴建枝,胡爱双,李全红   

  1. 河北工程大学 农学院,河北 邯郸 056002
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2011-09-25 发布日期:2011-09-25

Studies on maintaining virus\|free potato plantlets and optimization of microtuber induction

LIU Yan\|fen;CHEN Cui\|guo;LANG Wei\|ling;BAO Jian\|zhi;HU Ai\|shuang;LI Quan\|hong   

  1. College of Agriculture, Hebei Engineering University, Handan 056002, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-09-25 Published:2011-09-25

摘要: 研究了不同外源激素组合对马铃薯脱毒试管苗保苗效果的影响,研究了培养基、温度、光周期的综合作用对试管薯发生的影响。试验表明,适合于试管苗保苗的液体培养基为MS+CCC 4.0 mg·L-1+6-BA 0.5 mg·L-1+GA3 0.05 mg·L-1,适宜试管薯诱导的培养基和培养条件为:液体培养基MS+6-BA 6.0 mg·L-1+白糖60 g·L-1+活性炭3 g·L-1,环境温度18℃,光照10 h·d-1。

关键词: 马铃薯试管苗, 保苗, 试管薯, 优化

Abstract: The effects of exogenous hormone on maintaining of virus\|free potato plantlets and the combined effects of medium, temperature and photoperiod on microtuber induction were studied. The results showed that the optimal medium of maintaining plantlets was MS+CCC 40 mg·L-1+6\|BA 05 mg·L-1+GA3 005 mg·L-1,the optimal media to induce microtubers was MS+6\|BA 60 mg·L-1+sugar 60 g·L-1+active carbon 3 g·L-1, temperature at 18℃, and brightness for 10 h·d-1.

Key words: potato plantlets, maintaining, microtuber, optimization