›› 2013, Vol. 25 ›› Issue (2): 0-212.

• 论文 •    

利用IF2群体定位油菜含油量和产量相关性状QTL

程爽1,2, 孙中永1,2, 黄吉祥2, 曹明富1, 赵坚义2,*   

  1. 1杭州师范大学 生命与环境科学学院,浙江 杭州310036;2浙江省农业科学院 作物与核技术利用研究所,浙江 杭州310021
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2013-03-25 发布日期:2013-03-25

Mapping QTL for oil content and yield-related traits in Brassica napus L.using immortalized F2 population

CHENG Shuang;SUN Zhong-yong;HUANG Ji-xiang;CAO Ming-fu;ZHAO Jian-yi;*   

  1. 1College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China; 2Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2013-03-25 Published:2013-03-25

摘要: 为探明甘蓝型油菜含油量和产量相关性状的遗传机理,利用Sollux/ Gaoyou DH群体株系间随机交配构建了包括134个组合的永久F2群体(immortalized F2 population)。采用QTL Network 20软件对含油量、千粒重和角果粒数进行了4个环境下的联合QTL定位,分析QTL的加性、显性以及相关的上位性效应。结果在7条连锁群上检测到控制上述3个性状相关QTL共 8个。除qSWC6和qSPA2,1个含油量,4个千粒重和1个角果粒数QTL与多环境下SG\|DH群体定位结果区间重叠,加性效应方向一致,效应值相仿;主效含油量QTL qOILA7和3个千粒重QTL qSWA7,qSWC2, qSWC4无显性效应;qSWC6兼具加性和显性效应,qSWC8加性为主,微效显性,显性效应均呈负向;但两个角果粒数QTL qSPA2 和qSPA6除加性主效外,还存在正向显性效应;4对上位互作QTL中,除qOILA7,其余7个位点均只有微弱互作效应。并对qOILA7和qSWA7/qSWC8连锁标记辅助选育提高油菜含油量和千粒重的育种策略进行了探讨。结果表明:油菜种子含油量和千粒重性状的遗传控制体系以加性效应为主,F1代无杂种优势,因而欲提高杂交油菜种子含油量和增加千粒重,需要培育高含油量和大粒亲本材料;而控制角果粒数基因同时具有较强的加性和正向显性效应,利用F1杂交种可望获得超双亲的杂种优势。

关键词: 甘蓝型油菜, 永久F2群体, QTL, 含油量, 千粒重, 角果粒数

Abstract: In order to study the genetic mechanism of oil content and yield-related traits in B. napus, an immortalized F2 (designated as IF2) population (134 crosses) was generated by randomly permutated intermating of SG-DH lines. Phenotypic data were collected from four environments. Using updated SG-map and QTL Network 20 mapping program, the QTL analysis for oil content (OIL), 1000-seed weight (SW) and seeds per silique (SP) were conducted. The genetic effects including additive, dominant and all related digenic epistasis were estimated. The results showed that in total eight major QTL for three traits were identified in seven linkage groups. Except qSWC6 and qSPA2,one QTL for OIL, four for SW and one for SP were all located in the overlapped confidence intervals with that revealed by SG\|DH population,showing similar additive effect and same effect direction. In the loci of qOILA7 and three of SW qSWA7,qSWC2,qSWC4, significant additive without dominant effects were found. While for qSWC6 and qSWC8, both additive and negative dominant effects were detected. However, two major QTL for SP (qSPA2 and qSPA6) showed positive dominant effects beside additive effects. Of eight loci corresponding to four pairs of digenic interactions, seven were marker loci without additive effects involved. The QTL-based selection for increasing oil content by flanking markers of qOILA7 and seed weight through linked markers with qSWA7/qSWC8 was discussed. The results showed that predominant additive effects were observed for QTL of oil content and 1000-seed weight, predicting no heterosis of these two traits in F1 generation. Therefore, to increase the oil content and 1000-seed weight in rapeseed hybrids, the strategy was to breed two parents with high oil content and large seed size first. However, since seeds per silique demonstrated both additive and positively dominant effects, the performance which was better than two parents could be expected in hybrids and might contribute to yield heterosis of F1 hybrids.

Key words: Brassica napus L., immortalized F2 population, QTL, oil content, 1000\|seed weight, seeds per silique