›› 2009, Vol. 21 ›› Issue (3): 0-201.

• 论文 •    

侵染水仙的虎眼万年青花叶病毒外壳蛋白的原核表达、抗血清制备及应用

郑红英,鲁宇文,沈嘉乐,林林,陈 炯,陈剑平*   

  1. 浙江省农业科学院病毒学与生物技术研究所,浙江杭州310021
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-05-25 发布日期:2009-05-25

Prokauryotic expression of coat protein gene ofOrnithogalum mosaic virusand its use in the antiserum preparation

ZHENG Hong-ying;LU Yu-wen;SHEN Jia-le;LIN lin;CHEN Jiong;CHEN Jian-ping*   

  1. Institute of Virology and Biotechnology,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-05-25 Published:2009-05-25

摘要: 采用特异性表达引物PCR扩增了一个从英国围裙水仙样品上分离的虎眼万年青花叶病毒外壳蛋白
基因序列。构建原核表达载体,制备了OrMV分离物的外壳蛋白抗血清。对制备的抗血清进行了Western-blot检测,血清学关系研究并应用于样品的间接ELISA检测。

关键词: 虎眼万年青花叶病毒, 原核表达, 抗血清制备, 间接ELISA

Abstract: Coat protein gene of Ornithogalum mosaic virus(OrMV)was amplified from a virus infected Narcissus bulbocodium sample from UK by RT-PCR.The coat protein of OrMV was prokatyofic expressed,purified and used in
preparation of antisenun againstthe virus.OrMV then was detected in local and imported narcissussamples using this antiserum by an indirect ELISA method, and serological relationship between OrMV and other narcissus viruses were discussed.

Key words: Ornithogalum mosaic virus(OrMV), prokaryofic expression, antiserum preparation, indirect EIJSA