›› 2014, Vol. 26 ›› Issue (1): 0-88.

• 园艺科学 •    下一篇

瓦松快繁体系的构建研究

李庆伟,侯殿明,梁明勤,刘荣宁   

  1. 河南农业职业学院 现代农业工程系,河南 郑州 45145
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2014-01-25 发布日期:2014-07-09

Construction of rapid propagation system for Orostachys fimbriatus

LI Qing\|wei;HOU Dian\|ming;LIANG Ming\|qin;LIU Rong\|ning   

  1. (Department of Modern Agricultural Engineering, Henan Vocational College o
  • Received:1900-01-01 Revised:1900-01-01 Online:2014-01-25 Published:2014-07-09

摘要: 以瓦松为研究材料, 系统地研究了丛生芽的诱导、增殖及生根培养等,探索瓦松快繁的最佳途径并构建其快繁体系,结果表明: 茎段接种于MS+10 mg·L-16\|BA+02 mg·L-1IBA+20 g·L-1蔗糖培养基上,茎段增殖倍数可达1169,叶片接种于MS+15 mg·L-16\|BA+01 mg·L-1IBA+30 g·L-1蔗糖培养基上,能产生很好的不定芽,其再生芽数达971。在1/2 MS+02 mg·L-1IBA+005 mg·L-1NAA培养基中,生根率达100%,平均每株能产生123条根,植株生长健壮。

关键词: 瓦松, 快繁, 丛生芽, 生根

Abstract: Studies on the multiple shoots induction, propagation and rooting of Orostachys fimbriatus plantlet in vitro were conducted to construct a rapid propagation system for Orostachys fimbriatus. The results were as follows: the proliferation multiple of the stem was 1169 when cultured with the MS+10mg·L-1 6\|BA+02mg·L -1 IBA+20g·L -1 sucrose medium. Leaves inoculated on MS+15mg·L -1 6\|BA+01mg·L-1 IBA+30g·L-1 sucrose medium could regenerate small adventitious buds well, and the number of regenerated bud per leaf was 971. The vigorous proliferous shoots were inoculated on 1/2 MS+02mg·L-1 IBA+005mg·L-1 NAA medium for rooting, and the rooting rate was 100%, the root number was 123 per shoot.

Key words: Orostachys fimbriatus, rapid propagation, multiple shoots, rooting