›› 2014, Vol. 26 ›› Issue (2): 0-384387.

• 植物保护 •    

三种海藻抑菌活性和抗氧化活性

钱卓权1,殷皓臻1,黄晓林2,林少珍2,南海函1,*   

  1. 1温州医学院 生命科学学院,温州 浙江 325035;2浙江省海洋水产养殖研究所,温州 浙江 325000
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2014-03-25 发布日期:2014-07-09

Antibacterial and antioxidant bioassay of three kinds of algae

QIAN Zhuoquan;YIN Haozhen;HUANG Xiaolin;LIN Shaozhen;NAN Haihan;*   

  1. 1 School of Life Sciences, Wenzhou Medical College, Wenzhou 325035, China; 2Zhejiang Mariculture Research Institute, Wenzhou 325000, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2014-03-25 Published:2014-07-09

摘要: 以药敏纸片扩散法检测3种大型海藻鼠尾藻(Sargassum thunbergii)、羊栖菜(Sargassum fusiforme)和海带(Laminaria japonica)提取物对大肠杆菌、金黄色葡萄球菌和铜绿假单胞菌的抑制作用,以DPPH清除自由基方法检测3种海藻提取物的抗氧化能力。结果表明,3种海藻提取物对金黄色葡萄球菌均有较显著抑制作用,且以鼠尾藻正丁醇提取物的抑菌效果最佳;鼠尾藻提取物抗氧化能力较强,其中乙酸乙酯提取物的抗氧化活性比石油醚和正丁醇提取物强。

关键词: 海藻, 抑菌活性, DPPH, 抗氧化能力

Abstract:

The antibacterial activity of extracts of Sargassum thunbergii, Sargassum fusiforme and Laminaria japonica against Colon bacillus, Staphylococcus aureus and Pseudomonas aeruginosa was tested by drugsensitive disk diffusion method. Antioxidant activities of these extracts were analyzed by DPPH free radical scavenging test. The extracts of three kinds of algae showed significant antibacterial activities against S. aureus. Nbutanol extracted from S. thunbergii had the highest antibacterial effects. DPPH tests revealed the extracts of S. thunbergii were more significantly antioxidant than extracts of other algae. And the antioxidant activity of the ethyl acetate extract of S. thunbergii was stronger than those of petroleum ether and nbutanol extracts of S. thunbergii.

Key words: algae, antibacterial activity, DPPH, antioxidant bioassay